Proteomic identification of protein associated to mature spermatozoa in the Pacific oyster Crassostrea gigas
International audience Knowledge of sperm maturation process is limited in the Pacific oyster Crassostrea gigas and major factors of fertilization success of this free spawning animal are unknown. We investigated proteins associated to spermatozoa by analyzing two cellular fractions obtained from a...
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ftnormandieuniv:oai:HAL:hal-02296503v1 2023-11-12T04:16:04+01:00 Proteomic identification of protein associated to mature spermatozoa in the Pacific oyster Crassostrea gigas Kingtong, Sutin Kellner, Kristell Bernay, Benoît Goux, Didier Sourdaine, Pascal Berthelin, Clothilde Heude Biologie des mollusques marins et des écosystèmes associés (BioMEA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS) Burapha University (BU) Interactions Cellules Organismes Environnement (ICORE) Normandie Université (NU)-Normandie Université (NU)-CHU Caen Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN) 2013-04 https://normandie-univ.hal.science/hal-02296503 https://doi.org/10.1016/j.jprot.2013.02.009 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jprot.2013.02.009 hal-02296503 https://normandie-univ.hal.science/hal-02296503 doi:10.1016/j.jprot.2013.02.009 ISSN: 1874-3919 EISSN: 1876-7737 Journal of Proteomics https://normandie-univ.hal.science/hal-02296503 Journal of Proteomics, 2013, 82, pp.81-91. ⟨10.1016/j.jprot.2013.02.009⟩ Pacific oyster Crassostrea gigas Spermatozoa Proteomics Percoll gradient [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2013 ftnormandieuniv https://doi.org/10.1016/j.jprot.2013.02.009 2023-10-17T22:47:49Z International audience Knowledge of sperm maturation process is limited in the Pacific oyster Crassostrea gigas and major factors of fertilization success of this free spawning animal are unknown. We investigated proteins associated to spermatozoa by analyzing two cellular fractions obtained from a 40–80% Percoll gradient fractioning of germ cell of mature male gonads. Mature spermatozoa were enriched in the lower Percoll fraction while the upper fraction contained less mature or earlier germ cells. A 2-DE proteomic approach was used to identify differentially expressed proteins in both fractions. We screened out 31 differential proteins (P < 0.05) which included 14 up-regulated and 17 down-regulated proteins. Using MALDI-TOF/TOF MS and bioinformatics search against a C. gigas database, 13 and 8 proteins were identified for the up-regulated and down-regulated groups, respectively. In the spermatozoa enriched fraction, proteins regarding flagellum formation and control, energy production and Proteosome subunit beta were increased. In less mature germ cell fraction, proteins regarding developmental processes and chaperon molecules were mainly increased. Our results improve current knowledge of proteins associated with spermatozoa maturation related to zootechnical practices used in mollusk hatcheries. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Normandie Université: HAL Pacific Journal of Proteomics 82 81 91 |
institution |
Open Polar |
collection |
Normandie Université: HAL |
op_collection_id |
ftnormandieuniv |
language |
English |
topic |
Pacific oyster Crassostrea gigas Spermatozoa Proteomics Percoll gradient [SDV]Life Sciences [q-bio] |
spellingShingle |
Pacific oyster Crassostrea gigas Spermatozoa Proteomics Percoll gradient [SDV]Life Sciences [q-bio] Kingtong, Sutin Kellner, Kristell Bernay, Benoît Goux, Didier Sourdaine, Pascal Berthelin, Clothilde Heude Proteomic identification of protein associated to mature spermatozoa in the Pacific oyster Crassostrea gigas |
topic_facet |
Pacific oyster Crassostrea gigas Spermatozoa Proteomics Percoll gradient [SDV]Life Sciences [q-bio] |
description |
International audience Knowledge of sperm maturation process is limited in the Pacific oyster Crassostrea gigas and major factors of fertilization success of this free spawning animal are unknown. We investigated proteins associated to spermatozoa by analyzing two cellular fractions obtained from a 40–80% Percoll gradient fractioning of germ cell of mature male gonads. Mature spermatozoa were enriched in the lower Percoll fraction while the upper fraction contained less mature or earlier germ cells. A 2-DE proteomic approach was used to identify differentially expressed proteins in both fractions. We screened out 31 differential proteins (P < 0.05) which included 14 up-regulated and 17 down-regulated proteins. Using MALDI-TOF/TOF MS and bioinformatics search against a C. gigas database, 13 and 8 proteins were identified for the up-regulated and down-regulated groups, respectively. In the spermatozoa enriched fraction, proteins regarding flagellum formation and control, energy production and Proteosome subunit beta were increased. In less mature germ cell fraction, proteins regarding developmental processes and chaperon molecules were mainly increased. Our results improve current knowledge of proteins associated with spermatozoa maturation related to zootechnical practices used in mollusk hatcheries. |
author2 |
Biologie des mollusques marins et des écosystèmes associés (BioMEA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS) Burapha University (BU) Interactions Cellules Organismes Environnement (ICORE) Normandie Université (NU)-Normandie Université (NU)-CHU Caen Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN) |
format |
Article in Journal/Newspaper |
author |
Kingtong, Sutin Kellner, Kristell Bernay, Benoît Goux, Didier Sourdaine, Pascal Berthelin, Clothilde Heude |
author_facet |
Kingtong, Sutin Kellner, Kristell Bernay, Benoît Goux, Didier Sourdaine, Pascal Berthelin, Clothilde Heude |
author_sort |
Kingtong, Sutin |
title |
Proteomic identification of protein associated to mature spermatozoa in the Pacific oyster Crassostrea gigas |
title_short |
Proteomic identification of protein associated to mature spermatozoa in the Pacific oyster Crassostrea gigas |
title_full |
Proteomic identification of protein associated to mature spermatozoa in the Pacific oyster Crassostrea gigas |
title_fullStr |
Proteomic identification of protein associated to mature spermatozoa in the Pacific oyster Crassostrea gigas |
title_full_unstemmed |
Proteomic identification of protein associated to mature spermatozoa in the Pacific oyster Crassostrea gigas |
title_sort |
proteomic identification of protein associated to mature spermatozoa in the pacific oyster crassostrea gigas |
publisher |
HAL CCSD |
publishDate |
2013 |
url |
https://normandie-univ.hal.science/hal-02296503 https://doi.org/10.1016/j.jprot.2013.02.009 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 1874-3919 EISSN: 1876-7737 Journal of Proteomics https://normandie-univ.hal.science/hal-02296503 Journal of Proteomics, 2013, 82, pp.81-91. ⟨10.1016/j.jprot.2013.02.009⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jprot.2013.02.009 hal-02296503 https://normandie-univ.hal.science/hal-02296503 doi:10.1016/j.jprot.2013.02.009 |
op_doi |
https://doi.org/10.1016/j.jprot.2013.02.009 |
container_title |
Journal of Proteomics |
container_volume |
82 |
container_start_page |
81 |
op_container_end_page |
91 |
_version_ |
1782333257240543232 |