DNA Methylation Is Crucial for the Early Development in the Oyster C. gigas
International audience In vertebrates, epigenetic modifications influence gene transcription, and an appropriate DNA methylation is critical in development. Indeed, a precise temporal and spatial pattern of early gene expression is mandatory for a normal embryogenesis. However, such a regulation and...
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ftnormandieuniv:oai:HAL:hal-02296498v1 2023-11-12T04:16:16+01:00 DNA Methylation Is Crucial for the Early Development in the Oyster C. gigas Riviere, Guillaume Wu, Guan-Chung Fellous, Alexandre Goux, Didier Sourdaine, Pascal Favrel, Pascal Biologie des mollusques marins et des écosystèmes associés (BioMEA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS) National Taiwan Ocean University (NTOU) Interactions Cellules Organismes Environnement (ICORE) Normandie Université (NU)-Normandie Université (NU)-CHU Caen Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN) 2013-12 https://normandie-univ.hal.science/hal-02296498 https://doi.org/10.1007/s10126-013-9523-2 en eng HAL CCSD Springer Verlag info:eu-repo/semantics/altIdentifier/doi/10.1007/s10126-013-9523-2 hal-02296498 https://normandie-univ.hal.science/hal-02296498 doi:10.1007/s10126-013-9523-2 ISSN: 1436-2228 EISSN: 1436-2236 Marine Biotechnology https://normandie-univ.hal.science/hal-02296498 Marine Biotechnology, 2013, 15 (6), pp.739-753. ⟨10.1007/s10126-013-9523-2⟩ Evolution Homeobox Methylation Development Epigenetics [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2013 ftnormandieuniv https://doi.org/10.1007/s10126-013-9523-2 2023-10-17T22:47:49Z International audience In vertebrates, epigenetic modifications influence gene transcription, and an appropriate DNA methylation is critical in development. Indeed, a precise temporal and spatial pattern of early gene expression is mandatory for a normal embryogenesis. However, such a regulation and its underlying mechanisms remain poorly understood in more distant organisms such as Lophotrochozoa. Thus, despite DNA in the oyster genome being methylated, the role of DNA methylation in development is unknown. To clarify this point, oyster genomic DNA was examined during early embryogenesis and found differentially methylated. Reverse transcriptase quantitative polymerase chain reaction indicated stage-specific levels of transcripts encoding DNA-methyltransferase (DNMT) and methyl-binding domain proteins. In addition, as highlighted by electronic microscopy and immunohistochemistry, the DNMT inhibitor 5-aza-cytidine induced alterations in the quantity and the localisation of methylated DNA and severe dose-dependent development alterations and was lethal after zygotic genome reinitiation. Furthermore, methyl-DNA-immunoprecipitation–quantitative polymerase chain reaction revealed that the transcription level of most of the homeobox gene orthologues examined, but not of the other early genes investigated, was inversely correlated with their specific DNA methylation. Altogether, our results demonstrate that DNA methylation influences gene expression in Crassostrea gigas and is critical for oyster development, possibly by specifically controlling the transcription level of homeobox orthologues. These findings provide evidence for the importance of epigenetic regulation of development in Lophotrochozoans and bring new insights into the early life of C. gigas, one of the most important aquaculture resources worldwide. Article in Journal/Newspaper Crassostrea gigas Normandie Université: HAL Marine Biotechnology 15 6 739 753 |
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Open Polar |
collection |
Normandie Université: HAL |
op_collection_id |
ftnormandieuniv |
language |
English |
topic |
Evolution Homeobox Methylation Development Epigenetics [SDV]Life Sciences [q-bio] |
spellingShingle |
Evolution Homeobox Methylation Development Epigenetics [SDV]Life Sciences [q-bio] Riviere, Guillaume Wu, Guan-Chung Fellous, Alexandre Goux, Didier Sourdaine, Pascal Favrel, Pascal DNA Methylation Is Crucial for the Early Development in the Oyster C. gigas |
topic_facet |
Evolution Homeobox Methylation Development Epigenetics [SDV]Life Sciences [q-bio] |
description |
International audience In vertebrates, epigenetic modifications influence gene transcription, and an appropriate DNA methylation is critical in development. Indeed, a precise temporal and spatial pattern of early gene expression is mandatory for a normal embryogenesis. However, such a regulation and its underlying mechanisms remain poorly understood in more distant organisms such as Lophotrochozoa. Thus, despite DNA in the oyster genome being methylated, the role of DNA methylation in development is unknown. To clarify this point, oyster genomic DNA was examined during early embryogenesis and found differentially methylated. Reverse transcriptase quantitative polymerase chain reaction indicated stage-specific levels of transcripts encoding DNA-methyltransferase (DNMT) and methyl-binding domain proteins. In addition, as highlighted by electronic microscopy and immunohistochemistry, the DNMT inhibitor 5-aza-cytidine induced alterations in the quantity and the localisation of methylated DNA and severe dose-dependent development alterations and was lethal after zygotic genome reinitiation. Furthermore, methyl-DNA-immunoprecipitation–quantitative polymerase chain reaction revealed that the transcription level of most of the homeobox gene orthologues examined, but not of the other early genes investigated, was inversely correlated with their specific DNA methylation. Altogether, our results demonstrate that DNA methylation influences gene expression in Crassostrea gigas and is critical for oyster development, possibly by specifically controlling the transcription level of homeobox orthologues. These findings provide evidence for the importance of epigenetic regulation of development in Lophotrochozoans and bring new insights into the early life of C. gigas, one of the most important aquaculture resources worldwide. |
author2 |
Biologie des mollusques marins et des écosystèmes associés (BioMEA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS) National Taiwan Ocean University (NTOU) Interactions Cellules Organismes Environnement (ICORE) Normandie Université (NU)-Normandie Université (NU)-CHU Caen Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN) |
format |
Article in Journal/Newspaper |
author |
Riviere, Guillaume Wu, Guan-Chung Fellous, Alexandre Goux, Didier Sourdaine, Pascal Favrel, Pascal |
author_facet |
Riviere, Guillaume Wu, Guan-Chung Fellous, Alexandre Goux, Didier Sourdaine, Pascal Favrel, Pascal |
author_sort |
Riviere, Guillaume |
title |
DNA Methylation Is Crucial for the Early Development in the Oyster C. gigas |
title_short |
DNA Methylation Is Crucial for the Early Development in the Oyster C. gigas |
title_full |
DNA Methylation Is Crucial for the Early Development in the Oyster C. gigas |
title_fullStr |
DNA Methylation Is Crucial for the Early Development in the Oyster C. gigas |
title_full_unstemmed |
DNA Methylation Is Crucial for the Early Development in the Oyster C. gigas |
title_sort |
dna methylation is crucial for the early development in the oyster c. gigas |
publisher |
HAL CCSD |
publishDate |
2013 |
url |
https://normandie-univ.hal.science/hal-02296498 https://doi.org/10.1007/s10126-013-9523-2 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
ISSN: 1436-2228 EISSN: 1436-2236 Marine Biotechnology https://normandie-univ.hal.science/hal-02296498 Marine Biotechnology, 2013, 15 (6), pp.739-753. ⟨10.1007/s10126-013-9523-2⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1007/s10126-013-9523-2 hal-02296498 https://normandie-univ.hal.science/hal-02296498 doi:10.1007/s10126-013-9523-2 |
op_doi |
https://doi.org/10.1007/s10126-013-9523-2 |
container_title |
Marine Biotechnology |
container_volume |
15 |
container_issue |
6 |
container_start_page |
739 |
op_container_end_page |
753 |
_version_ |
1782333402949615616 |