Sex-specific regulation of AMP-activated protein kinase (AMPK) in the Pacific oyster Crassostrea gigas.
International audience The hermaphrodite Pacific oyster Crassostrea gigas displays a high energy allocation to reproduction. We studied the expression of AMP-activated protein kinase (AMPK) during gametogenesis in the gonad and characterized the mRNA sequences of the AMPK subunits: the AMPK alpha mR...
Published in: | Biology of Reproduction |
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Online Access: | https://hal.univ-brest.fr/hal-00946775 https://doi.org/10.1095/biolreprod.113.109728 |
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ftnormandieuniv:oai:HAL:hal-00946775v1 2024-04-28T08:16:33+00:00 Sex-specific regulation of AMP-activated protein kinase (AMPK) in the Pacific oyster Crassostrea gigas. Guévélou, Eric Huvet, Arnaud Galindo-Sánchez, Clara E Milan, Massimo Quillien, Virgile Daniel, Jean-Yves Quéré, Claudie Boudry, Pierre Corporeau, Charlotte Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Centro de Investigacion Cientifica y de Education Superior de Ensenada Mexico (CICESE) Department of Comparative Biomedicine and Food Science Università degli Studi di Padova = University of Padua (Unipd) Physiologie et Ecophysiologie des Mollusques Marins (PE2M) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) 2013-10 https://hal.univ-brest.fr/hal-00946775 https://doi.org/10.1095/biolreprod.113.109728 en eng HAL CCSD Society for the Study of Reproduction - Oxford Academic info:eu-repo/semantics/altIdentifier/doi/10.1095/biolreprod.113.109728 info:eu-repo/semantics/altIdentifier/pmid/23926284 hal-00946775 https://hal.univ-brest.fr/hal-00946775 doi:10.1095/biolreprod.113.109728 PUBMED: 23926284 ISSN: 0006-3363 EISSN: 1529-7268 Biology of Reproduction https://hal.univ-brest.fr/hal-00946775 Biology of Reproduction, 2013, 89 (4), pp.100. ⟨10.1095/biolreprod.113.109728⟩ [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2013 ftnormandieuniv https://doi.org/10.1095/biolreprod.113.109728 2024-04-11T00:25:26Z International audience The hermaphrodite Pacific oyster Crassostrea gigas displays a high energy allocation to reproduction. We studied the expression of AMP-activated protein kinase (AMPK) during gametogenesis in the gonad and characterized the mRNA sequences of the AMPK subunits: the AMPK alpha mRNA sequence was previously characterized; we identified AMPK beta, AMPK gamma, and mRNAs of putative AMPK-related targets following bioinformatics mining on existing genomic resources. We analyzed the mRNA expression of the AMPK alpha, beta, and gamma subunits in the gonads of male and female oysters through a reproductive cycle, and we quantified the mRNA expression of genes belonging to fatty acid and glucose metabolism. AMPK alpha mRNA levels were more abundant in males at the first stage of gametogenesis, when mitotic activity and the differentiation of germinal cells occur, and were always more abundant in males than in females. Some targets of fatty acid and glucose metabolism appeared to be correlated with the expression of AMPK subunits at the mRNA level. We then analyzed the sex-specific AMPK activity by measuring the phosphorylation of the catalytic AMPK alpha protein and its expression at the protein level. Both the amount of AMPK alpha protein and threonine 172 phosphorylation appeared to be almost totally inhibited in mature female gonads at stage 3, at the time when accumulation of reserves in oocytes was promoted, while it remained at a high level in mature spermatozoa. Its activation might play a sex-dependent role in the management of energy during gametogenesis in oyster. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Normandie Université: HAL Biology of Reproduction 89 4 |
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language |
English |
topic |
[SDE]Environmental Sciences |
spellingShingle |
[SDE]Environmental Sciences Guévélou, Eric Huvet, Arnaud Galindo-Sánchez, Clara E Milan, Massimo Quillien, Virgile Daniel, Jean-Yves Quéré, Claudie Boudry, Pierre Corporeau, Charlotte Sex-specific regulation of AMP-activated protein kinase (AMPK) in the Pacific oyster Crassostrea gigas. |
topic_facet |
[SDE]Environmental Sciences |
description |
International audience The hermaphrodite Pacific oyster Crassostrea gigas displays a high energy allocation to reproduction. We studied the expression of AMP-activated protein kinase (AMPK) during gametogenesis in the gonad and characterized the mRNA sequences of the AMPK subunits: the AMPK alpha mRNA sequence was previously characterized; we identified AMPK beta, AMPK gamma, and mRNAs of putative AMPK-related targets following bioinformatics mining on existing genomic resources. We analyzed the mRNA expression of the AMPK alpha, beta, and gamma subunits in the gonads of male and female oysters through a reproductive cycle, and we quantified the mRNA expression of genes belonging to fatty acid and glucose metabolism. AMPK alpha mRNA levels were more abundant in males at the first stage of gametogenesis, when mitotic activity and the differentiation of germinal cells occur, and were always more abundant in males than in females. Some targets of fatty acid and glucose metabolism appeared to be correlated with the expression of AMPK subunits at the mRNA level. We then analyzed the sex-specific AMPK activity by measuring the phosphorylation of the catalytic AMPK alpha protein and its expression at the protein level. Both the amount of AMPK alpha protein and threonine 172 phosphorylation appeared to be almost totally inhibited in mature female gonads at stage 3, at the time when accumulation of reserves in oocytes was promoted, while it remained at a high level in mature spermatozoa. Its activation might play a sex-dependent role in the management of energy during gametogenesis in oyster. |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Centro de Investigacion Cientifica y de Education Superior de Ensenada Mexico (CICESE) Department of Comparative Biomedicine and Food Science Università degli Studi di Padova = University of Padua (Unipd) Physiologie et Ecophysiologie des Mollusques Marins (PE2M) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Guévélou, Eric Huvet, Arnaud Galindo-Sánchez, Clara E Milan, Massimo Quillien, Virgile Daniel, Jean-Yves Quéré, Claudie Boudry, Pierre Corporeau, Charlotte |
author_facet |
Guévélou, Eric Huvet, Arnaud Galindo-Sánchez, Clara E Milan, Massimo Quillien, Virgile Daniel, Jean-Yves Quéré, Claudie Boudry, Pierre Corporeau, Charlotte |
author_sort |
Guévélou, Eric |
title |
Sex-specific regulation of AMP-activated protein kinase (AMPK) in the Pacific oyster Crassostrea gigas. |
title_short |
Sex-specific regulation of AMP-activated protein kinase (AMPK) in the Pacific oyster Crassostrea gigas. |
title_full |
Sex-specific regulation of AMP-activated protein kinase (AMPK) in the Pacific oyster Crassostrea gigas. |
title_fullStr |
Sex-specific regulation of AMP-activated protein kinase (AMPK) in the Pacific oyster Crassostrea gigas. |
title_full_unstemmed |
Sex-specific regulation of AMP-activated protein kinase (AMPK) in the Pacific oyster Crassostrea gigas. |
title_sort |
sex-specific regulation of amp-activated protein kinase (ampk) in the pacific oyster crassostrea gigas. |
publisher |
HAL CCSD |
publishDate |
2013 |
url |
https://hal.univ-brest.fr/hal-00946775 https://doi.org/10.1095/biolreprod.113.109728 |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 0006-3363 EISSN: 1529-7268 Biology of Reproduction https://hal.univ-brest.fr/hal-00946775 Biology of Reproduction, 2013, 89 (4), pp.100. ⟨10.1095/biolreprod.113.109728⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1095/biolreprod.113.109728 info:eu-repo/semantics/altIdentifier/pmid/23926284 hal-00946775 https://hal.univ-brest.fr/hal-00946775 doi:10.1095/biolreprod.113.109728 PUBMED: 23926284 |
op_doi |
https://doi.org/10.1095/biolreprod.113.109728 |
container_title |
Biology of Reproduction |
container_volume |
89 |
container_issue |
4 |
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1797581627813003264 |