Techniques for delivery of arachidonic acid to Pacific oyster, Crassostrea gigas, spat
International audience The present study tested two techniques for dietary supplementation of Crassostrea gigas spat with PUFA, such as arachidonic acid (AA). The first technique consisted of a preliminary enrichment and growth of an algal concentrate (T-ISO, Isochrysis sp.) with AA dissolved in an...
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ftnormandieuniv:oai:HAL:hal-00670296v1 2024-04-28T08:16:34+00:00 Techniques for delivery of arachidonic acid to Pacific oyster, Crassostrea gigas, spat Séguineau, Catherine Soudant, Philippe Moal, Jeanne Delaporte, Maryse Miner, Philippe Quéré, Claudie Physiologie et Ecophysiologie des Mollusques Marins (PE2M) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) 2005 https://hal.univ-brest.fr/hal-00670296 https://doi.org/10.1007/s11745-005-1454-5 en eng HAL CCSD Springer Verlag info:eu-repo/semantics/altIdentifier/doi/10.1007/s11745-005-1454-5 hal-00670296 https://hal.univ-brest.fr/hal-00670296 doi:10.1007/s11745-005-1454-5 ISSN: 0024-4201 EISSN: 1558-9307 Lipids https://hal.univ-brest.fr/hal-00670296 Lipids, 2005, 40 (9), pp.931-939. ⟨10.1007/s11745-005-1454-5⟩ [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2005 ftnormandieuniv https://doi.org/10.1007/s11745-005-1454-5 2024-04-11T00:25:26Z International audience The present study tested two techniques for dietary supplementation of Crassostrea gigas spat with PUFA, such as arachidonic acid (AA). The first technique consisted of a preliminary enrichment and growth of an algal concentrate (T-ISO, Isochrysis sp.) with AA dissolved in an ethanol solution, the whole culture then being fed to the spat. This enrichment increased the AA weight percentage in T-ISO neutral and polar lipids from 0.6 to 22.4% and from 0.4 to 6.8%, respectively. The second delivery technique was direct addition separately of free AA dissolved in ethanol solution and algal concentrate (T-ISO + AA) to the spat-rearing tank. To test the efficiency of these delivery techniques, oyster spat were supplemented with AA-enriched T-ISO, T-ISO + AA, and T-ISO alone. The possible biological impacts of these dietary treatments were assessed by measuring growth, condition index, and TAG content of oyster spat. Dry weight and condition index of spat fed AA-enriched T-ISO decreased by 24 and 49%, respectively, after 26 d of feeding; basically, TAG content declined 88% after 34 d of conditioning. When AA was added directly to seawater, spat growth and condition index were comparable with those of oysters fed T-ISO alone. AA incorporation in oyster tissues was assessed by analysis of the FA compositions in both neutral and polar lipid fractions. After 34 cl, AA content in neutral lipids reached 7 and 11.7% in the spat fed, respectively, AA-enriched T-ISO and T-ISO + AA, as compared with 1.1% in spat fed only T-ISO. AA incorporation was greater in polar lipids than in neutral lipids, reaching 7.8 and 12.5% in spat fed AA-enriched T-ISO and T-ISO + AA, respectively. A direct addition of PUFA along with the food supply represents an effective and promising means to supplement PUFA to oyster spat. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Normandie Université: HAL Lipids 40 9 931 939 |
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Normandie Université: HAL |
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ftnormandieuniv |
language |
English |
topic |
[SDE]Environmental Sciences |
spellingShingle |
[SDE]Environmental Sciences Séguineau, Catherine Soudant, Philippe Moal, Jeanne Delaporte, Maryse Miner, Philippe Quéré, Claudie Techniques for delivery of arachidonic acid to Pacific oyster, Crassostrea gigas, spat |
topic_facet |
[SDE]Environmental Sciences |
description |
International audience The present study tested two techniques for dietary supplementation of Crassostrea gigas spat with PUFA, such as arachidonic acid (AA). The first technique consisted of a preliminary enrichment and growth of an algal concentrate (T-ISO, Isochrysis sp.) with AA dissolved in an ethanol solution, the whole culture then being fed to the spat. This enrichment increased the AA weight percentage in T-ISO neutral and polar lipids from 0.6 to 22.4% and from 0.4 to 6.8%, respectively. The second delivery technique was direct addition separately of free AA dissolved in ethanol solution and algal concentrate (T-ISO + AA) to the spat-rearing tank. To test the efficiency of these delivery techniques, oyster spat were supplemented with AA-enriched T-ISO, T-ISO + AA, and T-ISO alone. The possible biological impacts of these dietary treatments were assessed by measuring growth, condition index, and TAG content of oyster spat. Dry weight and condition index of spat fed AA-enriched T-ISO decreased by 24 and 49%, respectively, after 26 d of feeding; basically, TAG content declined 88% after 34 d of conditioning. When AA was added directly to seawater, spat growth and condition index were comparable with those of oysters fed T-ISO alone. AA incorporation in oyster tissues was assessed by analysis of the FA compositions in both neutral and polar lipid fractions. After 34 cl, AA content in neutral lipids reached 7 and 11.7% in the spat fed, respectively, AA-enriched T-ISO and T-ISO + AA, as compared with 1.1% in spat fed only T-ISO. AA incorporation was greater in polar lipids than in neutral lipids, reaching 7.8 and 12.5% in spat fed AA-enriched T-ISO and T-ISO + AA, respectively. A direct addition of PUFA along with the food supply represents an effective and promising means to supplement PUFA to oyster spat. |
author2 |
Physiologie et Ecophysiologie des Mollusques Marins (PE2M) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Séguineau, Catherine Soudant, Philippe Moal, Jeanne Delaporte, Maryse Miner, Philippe Quéré, Claudie |
author_facet |
Séguineau, Catherine Soudant, Philippe Moal, Jeanne Delaporte, Maryse Miner, Philippe Quéré, Claudie |
author_sort |
Séguineau, Catherine |
title |
Techniques for delivery of arachidonic acid to Pacific oyster, Crassostrea gigas, spat |
title_short |
Techniques for delivery of arachidonic acid to Pacific oyster, Crassostrea gigas, spat |
title_full |
Techniques for delivery of arachidonic acid to Pacific oyster, Crassostrea gigas, spat |
title_fullStr |
Techniques for delivery of arachidonic acid to Pacific oyster, Crassostrea gigas, spat |
title_full_unstemmed |
Techniques for delivery of arachidonic acid to Pacific oyster, Crassostrea gigas, spat |
title_sort |
techniques for delivery of arachidonic acid to pacific oyster, crassostrea gigas, spat |
publisher |
HAL CCSD |
publishDate |
2005 |
url |
https://hal.univ-brest.fr/hal-00670296 https://doi.org/10.1007/s11745-005-1454-5 |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 0024-4201 EISSN: 1558-9307 Lipids https://hal.univ-brest.fr/hal-00670296 Lipids, 2005, 40 (9), pp.931-939. ⟨10.1007/s11745-005-1454-5⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1007/s11745-005-1454-5 hal-00670296 https://hal.univ-brest.fr/hal-00670296 doi:10.1007/s11745-005-1454-5 |
op_doi |
https://doi.org/10.1007/s11745-005-1454-5 |
container_title |
Lipids |
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40 |
container_issue |
9 |
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931 |
op_container_end_page |
939 |
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