Changes in biochemical and hemocyte parameters of the Pacific oysters Crassostrea gigas fed T-Iso supplemented with lipid emulsions rich in eicosapentaenoic acid
International audience The aim of this study was to assess the effect of dietary eicosapentaenoic acid (20:5n-3) on hemocyte parameters such as hemocyte concentration, phagocytosis, and non-stimulated reactive oxygen species (ROS) production in Pacific oysters Crassostrea gigas, as well on proximate...
Published in: | Journal of Experimental Marine Biology and Ecology |
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Main Authors: | , , , , , , |
Other Authors: | , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2007
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Online Access: | https://hal.univ-brest.fr/hal-00667073 https://doi.org/10.1016/j.jembe.2006.12.021 |
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ftnormandieuniv:oai:HAL:hal-00667073v1 |
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openpolar |
institution |
Open Polar |
collection |
Normandie Université: HAL |
op_collection_id |
ftnormandieuniv |
language |
English |
topic |
Bivalves Crassostrea gigas Eicosapentaenoic acid (20:5n-3) Hemocytes Immunology Polyunsaturated fatty acids [SDE]Environmental Sciences |
spellingShingle |
Bivalves Crassostrea gigas Eicosapentaenoic acid (20:5n-3) Hemocytes Immunology Polyunsaturated fatty acids [SDE]Environmental Sciences Delaporte, Maryse Chu, Fu-Lin, Evelyn Langdon, Chris Moal, Jeanne Lambert, Christophe Samain, Jean-François Soudant, Philippe Changes in biochemical and hemocyte parameters of the Pacific oysters Crassostrea gigas fed T-Iso supplemented with lipid emulsions rich in eicosapentaenoic acid |
topic_facet |
Bivalves Crassostrea gigas Eicosapentaenoic acid (20:5n-3) Hemocytes Immunology Polyunsaturated fatty acids [SDE]Environmental Sciences |
description |
International audience The aim of this study was to assess the effect of dietary eicosapentaenoic acid (20:5n-3) on hemocyte parameters such as hemocyte concentration, phagocytosis, and non-stimulated reactive oxygen species (ROS) production in Pacific oysters Crassostrea gigas, as well on proximate biochemical and fatty acid compositions. One-year-old oysters (C. gigas) were fed T-Isochrysis aff. galbana (T-Iso), which is low in 20:5n-3, either alone or with supplements of a lipid emulsion rich in 20:5n-3 at 1%, 10% or 50% (dry weight of the algal ration) for up to 7 weeks. Changes in gill fatty acid composition demonstrated that the lipid emulsion was well ingested by oysters during the dietary conditioning. Biochemical analysis indicated that oysters fed supplements of 50% and, to a lesser extent, 10% lipid emulsions had a higher total lipid content compared with oysters fed other diets, suggesting a more advanced reproductive status for the oysters fed high doses of lipid emulsion. Moreover, some oysters in these two treatment groups spawned during the last three weeks of the seven-week feeding experiment. Lipid supplements had a significant influence on hemocyte concentration, phagocytic index and non-stimulated hemocyte ROS production. After 4 weeks, highest hemocyte concentrations were found in oysters fed on a supplement of 50% lipid emulsion compared with those fed on other diets but the hemocytes derived from these oysters had the lowest short-term phagocytic index. After 7 weeks of dietary conditioning, the ROS production in non-stimulated hemocytes of oysters fed 10% and 50% lipid emulsion declined. These results suggested that 20:5n-3, and perhaps its eicosanoid metabolites, affected oyster hemocyte functions; however, the reproductive status of oysters may also have interfered with the 20:5n-3 dietary effect. |
author2 |
Physiologie et Ecophysiologie des Mollusques Marins (PE2M) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) Virginia Institute of Marine Science (VIMS) Coastal Oregon Marine Experimental Station Oregon State University (OSU) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Delaporte, Maryse Chu, Fu-Lin, Evelyn Langdon, Chris Moal, Jeanne Lambert, Christophe Samain, Jean-François Soudant, Philippe |
author_facet |
Delaporte, Maryse Chu, Fu-Lin, Evelyn Langdon, Chris Moal, Jeanne Lambert, Christophe Samain, Jean-François Soudant, Philippe |
author_sort |
Delaporte, Maryse |
title |
Changes in biochemical and hemocyte parameters of the Pacific oysters Crassostrea gigas fed T-Iso supplemented with lipid emulsions rich in eicosapentaenoic acid |
title_short |
Changes in biochemical and hemocyte parameters of the Pacific oysters Crassostrea gigas fed T-Iso supplemented with lipid emulsions rich in eicosapentaenoic acid |
title_full |
Changes in biochemical and hemocyte parameters of the Pacific oysters Crassostrea gigas fed T-Iso supplemented with lipid emulsions rich in eicosapentaenoic acid |
title_fullStr |
Changes in biochemical and hemocyte parameters of the Pacific oysters Crassostrea gigas fed T-Iso supplemented with lipid emulsions rich in eicosapentaenoic acid |
title_full_unstemmed |
Changes in biochemical and hemocyte parameters of the Pacific oysters Crassostrea gigas fed T-Iso supplemented with lipid emulsions rich in eicosapentaenoic acid |
title_sort |
changes in biochemical and hemocyte parameters of the pacific oysters crassostrea gigas fed t-iso supplemented with lipid emulsions rich in eicosapentaenoic acid |
publisher |
HAL CCSD |
publishDate |
2007 |
url |
https://hal.univ-brest.fr/hal-00667073 https://doi.org/10.1016/j.jembe.2006.12.021 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
ISSN: 0022-0981 Journal of Experimental Marine Biology and Ecology https://hal.univ-brest.fr/hal-00667073 Journal of Experimental Marine Biology and Ecology, 2007, 343 (2), pp.261-275. ⟨10.1016/j.jembe.2006.12.021⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jembe.2006.12.021 hal-00667073 https://hal.univ-brest.fr/hal-00667073 doi:10.1016/j.jembe.2006.12.021 |
op_doi |
https://doi.org/10.1016/j.jembe.2006.12.021 |
container_title |
Journal of Experimental Marine Biology and Ecology |
container_volume |
343 |
container_issue |
2 |
container_start_page |
261 |
op_container_end_page |
275 |
_version_ |
1802643510864642048 |
spelling |
ftnormandieuniv:oai:HAL:hal-00667073v1 2024-06-23T07:52:15+00:00 Changes in biochemical and hemocyte parameters of the Pacific oysters Crassostrea gigas fed T-Iso supplemented with lipid emulsions rich in eicosapentaenoic acid Delaporte, Maryse Chu, Fu-Lin, Evelyn Langdon, Chris Moal, Jeanne Lambert, Christophe Samain, Jean-François Soudant, Philippe Physiologie et Ecophysiologie des Mollusques Marins (PE2M) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) Virginia Institute of Marine Science (VIMS) Coastal Oregon Marine Experimental Station Oregon State University (OSU) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) 2007 https://hal.univ-brest.fr/hal-00667073 https://doi.org/10.1016/j.jembe.2006.12.021 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jembe.2006.12.021 hal-00667073 https://hal.univ-brest.fr/hal-00667073 doi:10.1016/j.jembe.2006.12.021 ISSN: 0022-0981 Journal of Experimental Marine Biology and Ecology https://hal.univ-brest.fr/hal-00667073 Journal of Experimental Marine Biology and Ecology, 2007, 343 (2), pp.261-275. ⟨10.1016/j.jembe.2006.12.021⟩ Bivalves Crassostrea gigas Eicosapentaenoic acid (20:5n-3) Hemocytes Immunology Polyunsaturated fatty acids [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2007 ftnormandieuniv https://doi.org/10.1016/j.jembe.2006.12.021 2024-06-03T23:57:48Z International audience The aim of this study was to assess the effect of dietary eicosapentaenoic acid (20:5n-3) on hemocyte parameters such as hemocyte concentration, phagocytosis, and non-stimulated reactive oxygen species (ROS) production in Pacific oysters Crassostrea gigas, as well on proximate biochemical and fatty acid compositions. One-year-old oysters (C. gigas) were fed T-Isochrysis aff. galbana (T-Iso), which is low in 20:5n-3, either alone or with supplements of a lipid emulsion rich in 20:5n-3 at 1%, 10% or 50% (dry weight of the algal ration) for up to 7 weeks. Changes in gill fatty acid composition demonstrated that the lipid emulsion was well ingested by oysters during the dietary conditioning. Biochemical analysis indicated that oysters fed supplements of 50% and, to a lesser extent, 10% lipid emulsions had a higher total lipid content compared with oysters fed other diets, suggesting a more advanced reproductive status for the oysters fed high doses of lipid emulsion. Moreover, some oysters in these two treatment groups spawned during the last three weeks of the seven-week feeding experiment. Lipid supplements had a significant influence on hemocyte concentration, phagocytic index and non-stimulated hemocyte ROS production. After 4 weeks, highest hemocyte concentrations were found in oysters fed on a supplement of 50% lipid emulsion compared with those fed on other diets but the hemocytes derived from these oysters had the lowest short-term phagocytic index. After 7 weeks of dietary conditioning, the ROS production in non-stimulated hemocytes of oysters fed 10% and 50% lipid emulsion declined. These results suggested that 20:5n-3, and perhaps its eicosanoid metabolites, affected oyster hemocyte functions; however, the reproductive status of oysters may also have interfered with the 20:5n-3 dietary effect. Article in Journal/Newspaper Crassostrea gigas Normandie Université: HAL Pacific Journal of Experimental Marine Biology and Ecology 343 2 261 275 |