In vivo RNA interference in oyster -vasa silencing inhibits germ cell development
International audience This study investigated the potential of RNA interference, which is technically challenging in bivalve mollusc species, to assess gene function in the oyster Crassostrea gigas. We designed dsRNA targeting the oyster vasa-like gene (Oyvlg), specifically expressed in oyster germ...
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ftnormandieuniv:oai:HAL:hal-00663883v1 2024-04-28T08:16:22+00:00 In vivo RNA interference in oyster -vasa silencing inhibits germ cell development Fabioux, Caroline Corporeau, Charlotte Quillien, Virgile Favrel, Pascal Huvet, Arnaud Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Physiologie et Ecophysiologie des Mollusques Marins (PE2M) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) 2009 https://hal.univ-brest.fr/hal-00663883 https://doi.org/10.1111/j.1742-4658.2009.06982.x en eng HAL CCSD Wiley info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1742-4658.2009.06982.x hal-00663883 https://hal.univ-brest.fr/hal-00663883 doi:10.1111/j.1742-4658.2009.06982.x ISSN: 1742-464X EISSN: 1742-4658 FEBS Journal https://hal.univ-brest.fr/hal-00663883 FEBS Journal, 2009, 9, pp.2566-2573. ⟨10.1111/j.1742-4658.2009.06982.x⟩ Crassostrea gigas germline marine bivalve RNAi vasa [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2009 ftnormandieuniv https://doi.org/10.1111/j.1742-4658.2009.06982.x 2024-04-11T00:25:26Z International audience This study investigated the potential of RNA interference, which is technically challenging in bivalve mollusc species, to assess gene function in the oyster Crassostrea gigas. We designed dsRNA targeting the oyster vasa-like gene (Oyvlg), specifically expressed in oyster germ cells. In vivo injection of oyvl-dsRNA into the gonad provokes a knockdown phenotype corresponding to germ cell underproliferation and prematurely arrested meiosis througout the organ. The most severe phenotype observed is sterile. This knockdown phenotype is associated with a decrease in Oyvlg mRNA level of between 39% and 87%, and a strong reduction in OYVLG protein, to an undetectable level. Therefore, Oyvlg appears to be essential for germ cell development in Crassostrea gigas, particularly for mitotic proliferation and early meiosis. Our results demonstrate for the first time that in vivo RNA interference works efficiently in a bivalve species, opening major perspectives for functional genetic studies. Article in Journal/Newspaper Crassostrea gigas Normandie Université: HAL FEBS Journal 276 9 2566 2573 |
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Open Polar |
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Normandie Université: HAL |
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ftnormandieuniv |
language |
English |
topic |
Crassostrea gigas germline marine bivalve RNAi vasa [SDE]Environmental Sciences |
spellingShingle |
Crassostrea gigas germline marine bivalve RNAi vasa [SDE]Environmental Sciences Fabioux, Caroline Corporeau, Charlotte Quillien, Virgile Favrel, Pascal Huvet, Arnaud In vivo RNA interference in oyster -vasa silencing inhibits germ cell development |
topic_facet |
Crassostrea gigas germline marine bivalve RNAi vasa [SDE]Environmental Sciences |
description |
International audience This study investigated the potential of RNA interference, which is technically challenging in bivalve mollusc species, to assess gene function in the oyster Crassostrea gigas. We designed dsRNA targeting the oyster vasa-like gene (Oyvlg), specifically expressed in oyster germ cells. In vivo injection of oyvl-dsRNA into the gonad provokes a knockdown phenotype corresponding to germ cell underproliferation and prematurely arrested meiosis througout the organ. The most severe phenotype observed is sterile. This knockdown phenotype is associated with a decrease in Oyvlg mRNA level of between 39% and 87%, and a strong reduction in OYVLG protein, to an undetectable level. Therefore, Oyvlg appears to be essential for germ cell development in Crassostrea gigas, particularly for mitotic proliferation and early meiosis. Our results demonstrate for the first time that in vivo RNA interference works efficiently in a bivalve species, opening major perspectives for functional genetic studies. |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Physiologie et Ecophysiologie des Mollusques Marins (PE2M) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Fabioux, Caroline Corporeau, Charlotte Quillien, Virgile Favrel, Pascal Huvet, Arnaud |
author_facet |
Fabioux, Caroline Corporeau, Charlotte Quillien, Virgile Favrel, Pascal Huvet, Arnaud |
author_sort |
Fabioux, Caroline |
title |
In vivo RNA interference in oyster -vasa silencing inhibits germ cell development |
title_short |
In vivo RNA interference in oyster -vasa silencing inhibits germ cell development |
title_full |
In vivo RNA interference in oyster -vasa silencing inhibits germ cell development |
title_fullStr |
In vivo RNA interference in oyster -vasa silencing inhibits germ cell development |
title_full_unstemmed |
In vivo RNA interference in oyster -vasa silencing inhibits germ cell development |
title_sort |
in vivo rna interference in oyster -vasa silencing inhibits germ cell development |
publisher |
HAL CCSD |
publishDate |
2009 |
url |
https://hal.univ-brest.fr/hal-00663883 https://doi.org/10.1111/j.1742-4658.2009.06982.x |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
ISSN: 1742-464X EISSN: 1742-4658 FEBS Journal https://hal.univ-brest.fr/hal-00663883 FEBS Journal, 2009, 9, pp.2566-2573. ⟨10.1111/j.1742-4658.2009.06982.x⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1742-4658.2009.06982.x hal-00663883 https://hal.univ-brest.fr/hal-00663883 doi:10.1111/j.1742-4658.2009.06982.x |
op_doi |
https://doi.org/10.1111/j.1742-4658.2009.06982.x |
container_title |
FEBS Journal |
container_volume |
276 |
container_issue |
9 |
container_start_page |
2566 |
op_container_end_page |
2573 |
_version_ |
1797581527724326912 |