Toxicity to Bivalve Hemocytes of Pathogenic Vibrio Cytoplasmic Extract
International audience Using a chemiluminescence (CL) test, it had been previously demonstrated that Vibrio pectenicida, which is pathogenic to Pecten maximus larvae, was able to inhibit completely the CL activity of P. maximus hemocytes and partially inhibit those of Crassostrea gigas. Conversely,...
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ftnormandieuniv:oai:HAL:hal-00617005v1 2024-04-28T08:16:28+00:00 Toxicity to Bivalve Hemocytes of Pathogenic Vibrio Cytoplasmic Extract Lambert, Christophe Nicolas, Jean-Louis Bultel, Valérie Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Université européenne de Bretagne - European University of Brittany (UEB) Physiologie et Ecophysiologie des Mollusques Marins (PE2M) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) Laboratoire de chimie des substances naturelles : structure, synthèse et bio-activités des substances naturelles Muséum national d'Histoire naturelle (MNHN)-Centre National de la Recherche Scientifique (CNRS) 2003 https://hal.science/hal-00617005 https://hal.science/hal-00617005/document https://hal.science/hal-00617005/file/Lambert_et_al_2003_Journal_of_Invertebrate_Pathology_77_165-172_-auteurs.pdf en eng HAL CCSD Elsevier hal-00617005 https://hal.science/hal-00617005 https://hal.science/hal-00617005/document https://hal.science/hal-00617005/file/Lambert_et_al_2003_Journal_of_Invertebrate_Pathology_77_165-172_-auteurs.pdf info:eu-repo/semantics/OpenAccess ISSN: 0022-2011 EISSN: 1096-0805 Journal of Invertebrate Pathology https://hal.science/hal-00617005 Journal of Invertebrate Pathology, 2003, 77, pp.165-172 Vibrio pectenicida Pecten maximus Crassostrea gigas chemiluminescence toxin hemocyte bivalves [SDV.BA]Life Sciences [q-bio]/Animal biology info:eu-repo/semantics/article Journal articles 2003 ftnormandieuniv 2024-04-11T00:26:24Z International audience Using a chemiluminescence (CL) test, it had been previously demonstrated that Vibrio pectenicida, which is pathogenic to Pecten maximus larvae, was able to inhibit completely the CL activity of P. maximus hemocytes and partially inhibit those of Crassostrea gigas. Conversely, a Vibrio sp. strain, S322, pathogenic to C. gigas larvae was more active in reducing the CL activity of oyster hemocytes than of scallop hemocytes. Using this same CL biotest, V. pectenicida and S322 cytoplasmic extracts were shown to reproduce CL inhibition while the cytoplasmic extract of a nonpathogenic strain (U1, Pseudoalteromonas) was without effect. Moreover, cytoplasmic extract as well as live V. pectenicida cells provoked, within a few hours, death of P. maximus hemocytes adhering to a glass slide. After partial purification, it was shown that toxic activities of V. pectenicida cytoplasmic extract was due to a toxin, named VHKT (for vibrio hemocyte- killer toxin), which is heat stable, acid and protease resistant, and less than 3 kDa in molecular weight. Attempts to purify VHKT by reverse-phase (C18) HPLC separated activity into the fraction eluted by water at a retention time of 4.02 min. Article in Journal/Newspaper Crassostrea gigas Normandie Université: HAL |
institution |
Open Polar |
collection |
Normandie Université: HAL |
op_collection_id |
ftnormandieuniv |
language |
English |
topic |
Vibrio pectenicida Pecten maximus Crassostrea gigas chemiluminescence toxin hemocyte bivalves [SDV.BA]Life Sciences [q-bio]/Animal biology |
spellingShingle |
Vibrio pectenicida Pecten maximus Crassostrea gigas chemiluminescence toxin hemocyte bivalves [SDV.BA]Life Sciences [q-bio]/Animal biology Lambert, Christophe Nicolas, Jean-Louis Bultel, Valérie Toxicity to Bivalve Hemocytes of Pathogenic Vibrio Cytoplasmic Extract |
topic_facet |
Vibrio pectenicida Pecten maximus Crassostrea gigas chemiluminescence toxin hemocyte bivalves [SDV.BA]Life Sciences [q-bio]/Animal biology |
description |
International audience Using a chemiluminescence (CL) test, it had been previously demonstrated that Vibrio pectenicida, which is pathogenic to Pecten maximus larvae, was able to inhibit completely the CL activity of P. maximus hemocytes and partially inhibit those of Crassostrea gigas. Conversely, a Vibrio sp. strain, S322, pathogenic to C. gigas larvae was more active in reducing the CL activity of oyster hemocytes than of scallop hemocytes. Using this same CL biotest, V. pectenicida and S322 cytoplasmic extracts were shown to reproduce CL inhibition while the cytoplasmic extract of a nonpathogenic strain (U1, Pseudoalteromonas) was without effect. Moreover, cytoplasmic extract as well as live V. pectenicida cells provoked, within a few hours, death of P. maximus hemocytes adhering to a glass slide. After partial purification, it was shown that toxic activities of V. pectenicida cytoplasmic extract was due to a toxin, named VHKT (for vibrio hemocyte- killer toxin), which is heat stable, acid and protease resistant, and less than 3 kDa in molecular weight. Attempts to purify VHKT by reverse-phase (C18) HPLC separated activity into the fraction eluted by water at a retention time of 4.02 min. |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Université européenne de Bretagne - European University of Brittany (UEB) Physiologie et Ecophysiologie des Mollusques Marins (PE2M) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) Laboratoire de chimie des substances naturelles : structure, synthèse et bio-activités des substances naturelles Muséum national d'Histoire naturelle (MNHN)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Lambert, Christophe Nicolas, Jean-Louis Bultel, Valérie |
author_facet |
Lambert, Christophe Nicolas, Jean-Louis Bultel, Valérie |
author_sort |
Lambert, Christophe |
title |
Toxicity to Bivalve Hemocytes of Pathogenic Vibrio Cytoplasmic Extract |
title_short |
Toxicity to Bivalve Hemocytes of Pathogenic Vibrio Cytoplasmic Extract |
title_full |
Toxicity to Bivalve Hemocytes of Pathogenic Vibrio Cytoplasmic Extract |
title_fullStr |
Toxicity to Bivalve Hemocytes of Pathogenic Vibrio Cytoplasmic Extract |
title_full_unstemmed |
Toxicity to Bivalve Hemocytes of Pathogenic Vibrio Cytoplasmic Extract |
title_sort |
toxicity to bivalve hemocytes of pathogenic vibrio cytoplasmic extract |
publisher |
HAL CCSD |
publishDate |
2003 |
url |
https://hal.science/hal-00617005 https://hal.science/hal-00617005/document https://hal.science/hal-00617005/file/Lambert_et_al_2003_Journal_of_Invertebrate_Pathology_77_165-172_-auteurs.pdf |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
ISSN: 0022-2011 EISSN: 1096-0805 Journal of Invertebrate Pathology https://hal.science/hal-00617005 Journal of Invertebrate Pathology, 2003, 77, pp.165-172 |
op_relation |
hal-00617005 https://hal.science/hal-00617005 https://hal.science/hal-00617005/document https://hal.science/hal-00617005/file/Lambert_et_al_2003_Journal_of_Invertebrate_Pathology_77_165-172_-auteurs.pdf |
op_rights |
info:eu-repo/semantics/OpenAccess |
_version_ |
1797581591178903552 |