Sexually dimorphic transcription of estrogen receptors in cod gonads throughout a reproductive cycle
Author's accepted version (post-print). This manuscript has been accepted for publication in Journal of Molecular Endocrinology, but the version presented here has not yet been copy-edited, formatted or proofed. Consequently, BioScientifica accepts no responsibility for any errors or omissions...
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ftnorduniv:oai:nordopen.nord.no:11250/279299 2023-05-15T15:27:25+02:00 Sexually dimorphic transcription of estrogen receptors in cod gonads throughout a reproductive cycle Nagasawa, Kazue Presslauer, Christopher Kirtiklis, Lech Babiak, Igor Fernandes, Jorge M.O. 2015-03-14T07:20:26Z application/pdf http://hdl.handle.net/11250/279299 https://doi.org/10.1530/JME-13-0187 eng eng BioScientifica Nagasawa, K., Presslauer, C., Kirtiklis, L., Babiak, I. & Fernandes, J.M.O. (2014). Sexually dimorphic transcription of estrogen receptors in cod gonads throughout a reproductive cycle. Journal of Molecular Endocrinology , 52(3), 357-371. doi: urn:issn:1479-6813 http://hdl.handle.net/11250/279299 https://doi.org/10.1530/JME-13-0187 cristin:1129249 357-371 52 Journal of Molecular Endocrinology 3 VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Fish health: 923 Journal article Peer reviewed 2015 ftnorduniv https://doi.org/10.1530/JME-13-0187 2021-07-13T18:12:20Z Author's accepted version (post-print). This manuscript has been accepted for publication in Journal of Molecular Endocrinology, but the version presented here has not yet been copy-edited, formatted or proofed. Consequently, BioScientifica accepts no responsibility for any errors or omissions it may contain. The definitive version is now freely available at http://dx.doi.org/10.1530/JME-13-0187, 2014. The role of sex steroid regulation in gonadal maturation is a very complex process that is far from being fully understood. Hence, we have investigated seasonal changes in gonadal expression of estrogen receptors (ERs) in Atlantic cod (Gadus morhua L.), a batch spawner, throughout annual reproductive cycle. Three nuclear ER partial cDNA sequences (esr1, esr2a and esr2b) were cloned and all esr transcripts were detected mainly in liver and gonads of either sex. In situ hybridization of esrs along with germ cell (vasa) and gonadal somatic cell markers (gsdf, 3β-hsd and amh for testicular, or gsdf for ovarian somatic cells) showed that in testis all three esrs were preferentially localized within interstitial fibroblasts composed of immature and mature Leydig cells, whereas in ovary they were differentially expressed in both follicular cells and oocytes. Quantitative real-time PCR analysis revealed a sexually dimorphic expression pattern of the three esr paralogues in testis and ovary. A significant increase in esr2a expression was identified in testis and esr2b in ovary, whereas esr1 transcripts were elevated in both testis and ovary at February and March prior to the spawning period. The localization and sexually dimorphic expression of esr genes in gonads suggest a direct function of estrogen via ERs in gonadal somatic cell growth and differentiation for Leydig cell in testis and follicular cells in ovary throughout the annual reproductive cycle in Atlantic cod. Article in Journal/Newspaper atlantic cod Gadus morhua Open archive Nord universitet Vasa ENVELOPE(25.177,25.177,67.587,67.587) Journal of Molecular Endocrinology 52 3 357 371 |
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Open Polar |
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Open archive Nord universitet |
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ftnorduniv |
language |
English |
topic |
VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Fish health: 923 |
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VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Fish health: 923 Nagasawa, Kazue Presslauer, Christopher Kirtiklis, Lech Babiak, Igor Fernandes, Jorge M.O. Sexually dimorphic transcription of estrogen receptors in cod gonads throughout a reproductive cycle |
topic_facet |
VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Fish health: 923 |
description |
Author's accepted version (post-print). This manuscript has been accepted for publication in Journal of Molecular Endocrinology, but the version presented here has not yet been copy-edited, formatted or proofed. Consequently, BioScientifica accepts no responsibility for any errors or omissions it may contain. The definitive version is now freely available at http://dx.doi.org/10.1530/JME-13-0187, 2014. The role of sex steroid regulation in gonadal maturation is a very complex process that is far from being fully understood. Hence, we have investigated seasonal changes in gonadal expression of estrogen receptors (ERs) in Atlantic cod (Gadus morhua L.), a batch spawner, throughout annual reproductive cycle. Three nuclear ER partial cDNA sequences (esr1, esr2a and esr2b) were cloned and all esr transcripts were detected mainly in liver and gonads of either sex. In situ hybridization of esrs along with germ cell (vasa) and gonadal somatic cell markers (gsdf, 3β-hsd and amh for testicular, or gsdf for ovarian somatic cells) showed that in testis all three esrs were preferentially localized within interstitial fibroblasts composed of immature and mature Leydig cells, whereas in ovary they were differentially expressed in both follicular cells and oocytes. Quantitative real-time PCR analysis revealed a sexually dimorphic expression pattern of the three esr paralogues in testis and ovary. A significant increase in esr2a expression was identified in testis and esr2b in ovary, whereas esr1 transcripts were elevated in both testis and ovary at February and March prior to the spawning period. The localization and sexually dimorphic expression of esr genes in gonads suggest a direct function of estrogen via ERs in gonadal somatic cell growth and differentiation for Leydig cell in testis and follicular cells in ovary throughout the annual reproductive cycle in Atlantic cod. |
format |
Article in Journal/Newspaper |
author |
Nagasawa, Kazue Presslauer, Christopher Kirtiklis, Lech Babiak, Igor Fernandes, Jorge M.O. |
author_facet |
Nagasawa, Kazue Presslauer, Christopher Kirtiklis, Lech Babiak, Igor Fernandes, Jorge M.O. |
author_sort |
Nagasawa, Kazue |
title |
Sexually dimorphic transcription of estrogen receptors in cod gonads throughout a reproductive cycle |
title_short |
Sexually dimorphic transcription of estrogen receptors in cod gonads throughout a reproductive cycle |
title_full |
Sexually dimorphic transcription of estrogen receptors in cod gonads throughout a reproductive cycle |
title_fullStr |
Sexually dimorphic transcription of estrogen receptors in cod gonads throughout a reproductive cycle |
title_full_unstemmed |
Sexually dimorphic transcription of estrogen receptors in cod gonads throughout a reproductive cycle |
title_sort |
sexually dimorphic transcription of estrogen receptors in cod gonads throughout a reproductive cycle |
publisher |
BioScientifica |
publishDate |
2015 |
url |
http://hdl.handle.net/11250/279299 https://doi.org/10.1530/JME-13-0187 |
long_lat |
ENVELOPE(25.177,25.177,67.587,67.587) |
geographic |
Vasa |
geographic_facet |
Vasa |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
357-371 52 Journal of Molecular Endocrinology 3 |
op_relation |
Nagasawa, K., Presslauer, C., Kirtiklis, L., Babiak, I. & Fernandes, J.M.O. (2014). Sexually dimorphic transcription of estrogen receptors in cod gonads throughout a reproductive cycle. Journal of Molecular Endocrinology , 52(3), 357-371. doi: urn:issn:1479-6813 http://hdl.handle.net/11250/279299 https://doi.org/10.1530/JME-13-0187 cristin:1129249 |
op_doi |
https://doi.org/10.1530/JME-13-0187 |
container_title |
Journal of Molecular Endocrinology |
container_volume |
52 |
container_issue |
3 |
container_start_page |
357 |
op_container_end_page |
371 |
_version_ |
1766357863531282432 |