A transcriptomic analysis of diploid and triploid Atlantic salmon lenses with and without cataracts
To avoid negative environmental impacts of escapees and potential inter-breeding with wild populations, the Atlantic salmon farming industry has and continues to extensively test triploid fish that are sterile. However, they often show differences in performance, physiology, behavior and morphology...
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ftnorduniv:oai:nordopen.nord.no:11250/2728998 2023-05-15T15:30:17+02:00 A transcriptomic analysis of diploid and triploid Atlantic salmon lenses with and without cataracts Olsvik, Pål Asgeir Finn, Roderick Nigel Remø, Sofie C. Fjelldal, Per Gunnar Chauvigné, Francois Glover, Kevin Hansen, Tom Johnny Waagbø, Rune 2020 application/pdf https://hdl.handle.net/11250/2728998 https://doi.org/10.1016/j.exer.2020.108150 eng eng Elsevier Norges forskningsråd: 224816 Olsvik, P. A., Finn, R. N., Remø, S. C., Fjelldal, P. G., Chauvigné, F., Glover, K. A., Hansen, T. & Waagbø, R. (2020). A transcriptomic analysis of diploid and triploid Atlantic salmon lenses with and without cataracts. Experimental Eye Research, 199: 108150. doi: urn:issn:1096-0007 https://hdl.handle.net/11250/2728998 https://doi.org/10.1016/j.exer.2020.108150 cristin:1847035 Navngivelse 4.0 Internasjonal http://creativecommons.org/licenses/by/4.0/deed.no © 2020 The Author(s) CC-BY 14 199 Experimental Eye Research 108150 VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920 VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920::Akvakultur: 922 VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470 Peer reviewed Journal article 2020 ftnorduniv https://doi.org/10.1016/j.exer.2020.108150 2021-07-13T18:12:51Z To avoid negative environmental impacts of escapees and potential inter-breeding with wild populations, the Atlantic salmon farming industry has and continues to extensively test triploid fish that are sterile. However, they often show differences in performance, physiology, behavior and morphology compared to diploid fish, with increased prevalence of vertebral deformities and ocular cataracts as two of the most severe disorders. Here, we investigated the mechanisms behind the higher prevalence of cataracts in triploid salmon, by comparing the transcriptional patterns in lenses of diploid and triploid Atlantic salmon, with and without cataracts. We assembled and characterized the Atlantic salmon lens transcriptome and used RNA-seq to search for the molecular basis for cataract development in triploid fish. Transcriptional screening showed only modest differences in lens mRNA levels in diploid and triploid fish, with few uniquely expressed genes. In total, there were 165 differentially expressed genes (DEGs) between the cataractous diploid and triploid lens. Of these, most were expressed at lower levels in triploid fish. Differential expression was observed for genes encoding proteins with known function in the retina (phototransduction) and proteins associated with repair and compensation mechanisms. The results suggest a higher susceptibility to oxidative stress in triploid lenses, and that mechanisms connected to the ability to handle damaged proteins are differentially affected in cataractous lenses from diploid and triploid salmon. publishedVersion Unit Licence Agreement Article in Journal/Newspaper Atlantic salmon Open archive Nord universitet Experimental Eye Research 199 108150 |
institution |
Open Polar |
collection |
Open archive Nord universitet |
op_collection_id |
ftnorduniv |
language |
English |
topic |
VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920 VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920::Akvakultur: 922 VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470 |
spellingShingle |
VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920 VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920::Akvakultur: 922 VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470 Olsvik, Pål Asgeir Finn, Roderick Nigel Remø, Sofie C. Fjelldal, Per Gunnar Chauvigné, Francois Glover, Kevin Hansen, Tom Johnny Waagbø, Rune A transcriptomic analysis of diploid and triploid Atlantic salmon lenses with and without cataracts |
topic_facet |
VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920 VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920::Akvakultur: 922 VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470 |
description |
To avoid negative environmental impacts of escapees and potential inter-breeding with wild populations, the Atlantic salmon farming industry has and continues to extensively test triploid fish that are sterile. However, they often show differences in performance, physiology, behavior and morphology compared to diploid fish, with increased prevalence of vertebral deformities and ocular cataracts as two of the most severe disorders. Here, we investigated the mechanisms behind the higher prevalence of cataracts in triploid salmon, by comparing the transcriptional patterns in lenses of diploid and triploid Atlantic salmon, with and without cataracts. We assembled and characterized the Atlantic salmon lens transcriptome and used RNA-seq to search for the molecular basis for cataract development in triploid fish. Transcriptional screening showed only modest differences in lens mRNA levels in diploid and triploid fish, with few uniquely expressed genes. In total, there were 165 differentially expressed genes (DEGs) between the cataractous diploid and triploid lens. Of these, most were expressed at lower levels in triploid fish. Differential expression was observed for genes encoding proteins with known function in the retina (phototransduction) and proteins associated with repair and compensation mechanisms. The results suggest a higher susceptibility to oxidative stress in triploid lenses, and that mechanisms connected to the ability to handle damaged proteins are differentially affected in cataractous lenses from diploid and triploid salmon. publishedVersion Unit Licence Agreement |
format |
Article in Journal/Newspaper |
author |
Olsvik, Pål Asgeir Finn, Roderick Nigel Remø, Sofie C. Fjelldal, Per Gunnar Chauvigné, Francois Glover, Kevin Hansen, Tom Johnny Waagbø, Rune |
author_facet |
Olsvik, Pål Asgeir Finn, Roderick Nigel Remø, Sofie C. Fjelldal, Per Gunnar Chauvigné, Francois Glover, Kevin Hansen, Tom Johnny Waagbø, Rune |
author_sort |
Olsvik, Pål Asgeir |
title |
A transcriptomic analysis of diploid and triploid Atlantic salmon lenses with and without cataracts |
title_short |
A transcriptomic analysis of diploid and triploid Atlantic salmon lenses with and without cataracts |
title_full |
A transcriptomic analysis of diploid and triploid Atlantic salmon lenses with and without cataracts |
title_fullStr |
A transcriptomic analysis of diploid and triploid Atlantic salmon lenses with and without cataracts |
title_full_unstemmed |
A transcriptomic analysis of diploid and triploid Atlantic salmon lenses with and without cataracts |
title_sort |
transcriptomic analysis of diploid and triploid atlantic salmon lenses with and without cataracts |
publisher |
Elsevier |
publishDate |
2020 |
url |
https://hdl.handle.net/11250/2728998 https://doi.org/10.1016/j.exer.2020.108150 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
14 199 Experimental Eye Research 108150 |
op_relation |
Norges forskningsråd: 224816 Olsvik, P. A., Finn, R. N., Remø, S. C., Fjelldal, P. G., Chauvigné, F., Glover, K. A., Hansen, T. & Waagbø, R. (2020). A transcriptomic analysis of diploid and triploid Atlantic salmon lenses with and without cataracts. Experimental Eye Research, 199: 108150. doi: urn:issn:1096-0007 https://hdl.handle.net/11250/2728998 https://doi.org/10.1016/j.exer.2020.108150 cristin:1847035 |
op_rights |
Navngivelse 4.0 Internasjonal http://creativecommons.org/licenses/by/4.0/deed.no © 2020 The Author(s) |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1016/j.exer.2020.108150 |
container_title |
Experimental Eye Research |
container_volume |
199 |
container_start_page |
108150 |
_version_ |
1766360736935706624 |