Substantial Downregulation of Myogenic Transcripts in Skeletal Muscle of Atlantic Cod during the Spawning Period
Gonadal maturation is an extremely energy consuming process for batch spawners and it is associated with a significant decrease in growth and seasonal deterioration in flesh quality. Our knowledge about the molecular mechanisms linking sexual maturation and muscle growth is still limited. In the pre...
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ftnorduniv:oai:nordopen.nord.no:11250/2377350 2023-05-15T15:27:02+02:00 Substantial Downregulation of Myogenic Transcripts in Skeletal Muscle of Atlantic Cod during the Spawning Period Nagasawa, Kazue Sarropoulou, Elena Edvardsen, Vigdis Fernandes, Jorge M.O. 2016-02-05T07:45:30Z application/pdf http://hdl.handle.net/11250/2377350 https://doi.org/10.1371/journal.pone.0148374 eng eng Public Library of Science The Research Council of Norway: 190350 Nagasawa, K., Sarropoulou, E., Edvardsen, V. & Fernandes, J.M.O. (2016). Substantial downregulation of myogenic transcripts in skeletal muscle of Atlantic cod during the spawning period. PLoS ONE, 11(2). doi: urn:issn:1932-6203 http://hdl.handle.net/11250/2377350 https://doi.org/10.1371/journal.pone.0148374 cristin:1332464 Navngivelse 3.0 Norge http://creativecommons.org/licenses/by/3.0/no/ © 2016, The Author(s) CC-BY 21 11 PLoS ONE 2 VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920 VDP::Mathematics and natural science: 400::Basic biosciences: 470::Genetics and genomics: 474 Journal article Peer reviewed 2016 ftnorduniv https://doi.org/10.1371/journal.pone.0148374 2021-07-13T18:12:28Z Gonadal maturation is an extremely energy consuming process for batch spawners and it is associated with a significant decrease in growth and seasonal deterioration in flesh quality. Our knowledge about the molecular mechanisms linking sexual maturation and muscle growth is still limited. In the present study, we performed RNA-Seq using 454 GS-FLX pyrosequencing in fast skeletal muscle sampled from two-year-old Atlantic cod (Gadus morhua) at representative time points throughout the reproductive cycle (August, March and May). In total, 126,937 good quality reads were obtained, with 546 nucleotide length and 52% GC content on average. RNA-Seq analysis using the CLC Genomics Workbench with the Atlantic cod reference UniGene cDNA data revealed 59,581 (46.9%) uniquely annotated reads. Pairwise comparison for expression levels identified 153 differentially expressed UniGenes between time points. Notably, we found a significant suppression of myh13 and myofibrillar gene isoforms in fast skeletal muscle during the spawning season. This study uncovered a large number of differentially expressed genes that may be influenced by gonadal maturation, thus representing a significant contribution to our limited understanding of the molecular mechanisms regulating muscle wasting and regeneration in batch spawners during their reproductive cycle. Article in Journal/Newspaper atlantic cod Gadus morhua Open archive Nord universitet PLOS ONE 11 2 e0148374 |
institution |
Open Polar |
collection |
Open archive Nord universitet |
op_collection_id |
ftnorduniv |
language |
English |
topic |
VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920 VDP::Mathematics and natural science: 400::Basic biosciences: 470::Genetics and genomics: 474 |
spellingShingle |
VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920 VDP::Mathematics and natural science: 400::Basic biosciences: 470::Genetics and genomics: 474 Nagasawa, Kazue Sarropoulou, Elena Edvardsen, Vigdis Fernandes, Jorge M.O. Substantial Downregulation of Myogenic Transcripts in Skeletal Muscle of Atlantic Cod during the Spawning Period |
topic_facet |
VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920 VDP::Mathematics and natural science: 400::Basic biosciences: 470::Genetics and genomics: 474 |
description |
Gonadal maturation is an extremely energy consuming process for batch spawners and it is associated with a significant decrease in growth and seasonal deterioration in flesh quality. Our knowledge about the molecular mechanisms linking sexual maturation and muscle growth is still limited. In the present study, we performed RNA-Seq using 454 GS-FLX pyrosequencing in fast skeletal muscle sampled from two-year-old Atlantic cod (Gadus morhua) at representative time points throughout the reproductive cycle (August, March and May). In total, 126,937 good quality reads were obtained, with 546 nucleotide length and 52% GC content on average. RNA-Seq analysis using the CLC Genomics Workbench with the Atlantic cod reference UniGene cDNA data revealed 59,581 (46.9%) uniquely annotated reads. Pairwise comparison for expression levels identified 153 differentially expressed UniGenes between time points. Notably, we found a significant suppression of myh13 and myofibrillar gene isoforms in fast skeletal muscle during the spawning season. This study uncovered a large number of differentially expressed genes that may be influenced by gonadal maturation, thus representing a significant contribution to our limited understanding of the molecular mechanisms regulating muscle wasting and regeneration in batch spawners during their reproductive cycle. |
format |
Article in Journal/Newspaper |
author |
Nagasawa, Kazue Sarropoulou, Elena Edvardsen, Vigdis Fernandes, Jorge M.O. |
author_facet |
Nagasawa, Kazue Sarropoulou, Elena Edvardsen, Vigdis Fernandes, Jorge M.O. |
author_sort |
Nagasawa, Kazue |
title |
Substantial Downregulation of Myogenic Transcripts in Skeletal Muscle of Atlantic Cod during the Spawning Period |
title_short |
Substantial Downregulation of Myogenic Transcripts in Skeletal Muscle of Atlantic Cod during the Spawning Period |
title_full |
Substantial Downregulation of Myogenic Transcripts in Skeletal Muscle of Atlantic Cod during the Spawning Period |
title_fullStr |
Substantial Downregulation of Myogenic Transcripts in Skeletal Muscle of Atlantic Cod during the Spawning Period |
title_full_unstemmed |
Substantial Downregulation of Myogenic Transcripts in Skeletal Muscle of Atlantic Cod during the Spawning Period |
title_sort |
substantial downregulation of myogenic transcripts in skeletal muscle of atlantic cod during the spawning period |
publisher |
Public Library of Science |
publishDate |
2016 |
url |
http://hdl.handle.net/11250/2377350 https://doi.org/10.1371/journal.pone.0148374 |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
21 11 PLoS ONE 2 |
op_relation |
The Research Council of Norway: 190350 Nagasawa, K., Sarropoulou, E., Edvardsen, V. & Fernandes, J.M.O. (2016). Substantial downregulation of myogenic transcripts in skeletal muscle of Atlantic cod during the spawning period. PLoS ONE, 11(2). doi: urn:issn:1932-6203 http://hdl.handle.net/11250/2377350 https://doi.org/10.1371/journal.pone.0148374 cristin:1332464 |
op_rights |
Navngivelse 3.0 Norge http://creativecommons.org/licenses/by/3.0/no/ © 2016, The Author(s) |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1371/journal.pone.0148374 |
container_title |
PLOS ONE |
container_volume |
11 |
container_issue |
2 |
container_start_page |
e0148374 |
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1766357490045288448 |