Triploidy leads to a mismatch of smoltification biomarkers in the gill and differences in the optimal salinity for post-smolt growth in Atlantic salmon
Sterile triploid Atlantic salmon (Salmo salar) show inconsistent seawater grow-out, but the reason why remains unclear. The purpose of this study was to determine the salinity optima of triploid post-smolts. Diploids and triploids were assessed for smoltification status during an underyearling smolt...
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Online Access: | https://hdl.handle.net/11250/2985449 https://doi.org/10.1016/j.aquaculture.2021.737350 |
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ftnorce:oai:norceresearch.brage.unit.no:11250/2985449 2023-05-15T15:31:59+02:00 Triploidy leads to a mismatch of smoltification biomarkers in the gill and differences in the optimal salinity for post-smolt growth in Atlantic salmon de Fonseka, Raneesha Fjelldal, Per Gunnar Sambraus, Florian Nilsen, Tom Ole Remø, Sofie C. Stien, Lars Helge Reinardy, Helena Madaro, Angelico Hansen, Tom Johnny Fraser, Thomas 2021 application/pdf https://hdl.handle.net/11250/2985449 https://doi.org/10.1016/j.aquaculture.2021.737350 eng eng Havforskningsinstituttet: 14930-04 EC/H2020/652831 NORCE Norwegian Research Centre AS: AE150010 Aquaculture. 2021, 546 . urn:issn:0044-8486 https://hdl.handle.net/11250/2985449 https://doi.org/10.1016/j.aquaculture.2021.737350 cristin:2002872 Navngivelse 4.0 Internasjonal http://creativecommons.org/licenses/by/4.0/deed.no © 2021 The Author(s) CC-BY Aquaculture 546 13 Peer reviewed Journal article 2021 ftnorce https://doi.org/10.1016/j.aquaculture.2021.737350 2022-10-13T05:50:47Z Sterile triploid Atlantic salmon (Salmo salar) show inconsistent seawater grow-out, but the reason why remains unclear. The purpose of this study was to determine the salinity optima of triploid post-smolts. Diploids and triploids were assessed for smoltification status during an underyearling smolt regime before being transferred to one of four different salinities, 0, 11, 23 and 35 ppt at 12 °C and under 24 h continuous light for 83 days. During this period, fish growth, plasma biochemistry, and production traits (vertebral deformities, ocular cataracts, sexual maturation) were monitored. Molecular biomarkers in the gill (nkaα1a, nkaα1b, nkcc1a) suggested triploids reached peak smolt earlier than diploids and began the desmoltification process before the start of the salinity treatments, however this was not reflected in gill Na+/K+-ATPase enzyme activity. At the initiation of the salinity treatments triploids were significantly larger than diploids (mean weight g ± SE: 71 ± 0.7 and 87.2 ± 0.8 for diploids and triploids, respectively) and there was a ploidy effect on post-smolt growth, with body weight showing a clearer positive trend with salinity in diploids (0 < 11 = 23 = 35 ppt) than in triploids (0 < 11 < 35 = 23 ppt) (final mean weight g ± SE: 255.2 ± 7.4, 303.9 ± 9, 313.9 ± 9 and 342.4 ± 12 for diploids and 322.9 ± 9.7, 361.7 ± 10.7, 425.9 ± 12.1, 415.2 ± 12.2 for triploids at 0, 11, 23, and 35 ppt, respectively). Plasma Na+ and Cl− increased, but plasma pH decreased, with increasing salinity in both ploidy. However, ploidy only had transient effects on plasma biochemistry depending on the salinity treatment. There was no ploidy effect on vertebral deformities (21% of both ploidy had one or more deformed vertebra). In contrast, triploids had a significantly higher prevalence of ocular cataracts (84 vs 98% in diploids and triploids, respectively) with a higher mean cataract score (mean ± SE: 1.93 ± 0.1 and 2.78 ± 0.1 for diploids and triploids, respectively), but a significantly lower prevalence ... Article in Journal/Newspaper Atlantic salmon Salmo salar NORCE vitenarkiv (Norwegian Research Centre) Aquaculture 546 737350 |
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Open Polar |
collection |
NORCE vitenarkiv (Norwegian Research Centre) |
op_collection_id |
ftnorce |
language |
English |
description |
Sterile triploid Atlantic salmon (Salmo salar) show inconsistent seawater grow-out, but the reason why remains unclear. The purpose of this study was to determine the salinity optima of triploid post-smolts. Diploids and triploids were assessed for smoltification status during an underyearling smolt regime before being transferred to one of four different salinities, 0, 11, 23 and 35 ppt at 12 °C and under 24 h continuous light for 83 days. During this period, fish growth, plasma biochemistry, and production traits (vertebral deformities, ocular cataracts, sexual maturation) were monitored. Molecular biomarkers in the gill (nkaα1a, nkaα1b, nkcc1a) suggested triploids reached peak smolt earlier than diploids and began the desmoltification process before the start of the salinity treatments, however this was not reflected in gill Na+/K+-ATPase enzyme activity. At the initiation of the salinity treatments triploids were significantly larger than diploids (mean weight g ± SE: 71 ± 0.7 and 87.2 ± 0.8 for diploids and triploids, respectively) and there was a ploidy effect on post-smolt growth, with body weight showing a clearer positive trend with salinity in diploids (0 < 11 = 23 = 35 ppt) than in triploids (0 < 11 < 35 = 23 ppt) (final mean weight g ± SE: 255.2 ± 7.4, 303.9 ± 9, 313.9 ± 9 and 342.4 ± 12 for diploids and 322.9 ± 9.7, 361.7 ± 10.7, 425.9 ± 12.1, 415.2 ± 12.2 for triploids at 0, 11, 23, and 35 ppt, respectively). Plasma Na+ and Cl− increased, but plasma pH decreased, with increasing salinity in both ploidy. However, ploidy only had transient effects on plasma biochemistry depending on the salinity treatment. There was no ploidy effect on vertebral deformities (21% of both ploidy had one or more deformed vertebra). In contrast, triploids had a significantly higher prevalence of ocular cataracts (84 vs 98% in diploids and triploids, respectively) with a higher mean cataract score (mean ± SE: 1.93 ± 0.1 and 2.78 ± 0.1 for diploids and triploids, respectively), but a significantly lower prevalence ... |
format |
Article in Journal/Newspaper |
author |
de Fonseka, Raneesha Fjelldal, Per Gunnar Sambraus, Florian Nilsen, Tom Ole Remø, Sofie C. Stien, Lars Helge Reinardy, Helena Madaro, Angelico Hansen, Tom Johnny Fraser, Thomas |
spellingShingle |
de Fonseka, Raneesha Fjelldal, Per Gunnar Sambraus, Florian Nilsen, Tom Ole Remø, Sofie C. Stien, Lars Helge Reinardy, Helena Madaro, Angelico Hansen, Tom Johnny Fraser, Thomas Triploidy leads to a mismatch of smoltification biomarkers in the gill and differences in the optimal salinity for post-smolt growth in Atlantic salmon |
author_facet |
de Fonseka, Raneesha Fjelldal, Per Gunnar Sambraus, Florian Nilsen, Tom Ole Remø, Sofie C. Stien, Lars Helge Reinardy, Helena Madaro, Angelico Hansen, Tom Johnny Fraser, Thomas |
author_sort |
de Fonseka, Raneesha |
title |
Triploidy leads to a mismatch of smoltification biomarkers in the gill and differences in the optimal salinity for post-smolt growth in Atlantic salmon |
title_short |
Triploidy leads to a mismatch of smoltification biomarkers in the gill and differences in the optimal salinity for post-smolt growth in Atlantic salmon |
title_full |
Triploidy leads to a mismatch of smoltification biomarkers in the gill and differences in the optimal salinity for post-smolt growth in Atlantic salmon |
title_fullStr |
Triploidy leads to a mismatch of smoltification biomarkers in the gill and differences in the optimal salinity for post-smolt growth in Atlantic salmon |
title_full_unstemmed |
Triploidy leads to a mismatch of smoltification biomarkers in the gill and differences in the optimal salinity for post-smolt growth in Atlantic salmon |
title_sort |
triploidy leads to a mismatch of smoltification biomarkers in the gill and differences in the optimal salinity for post-smolt growth in atlantic salmon |
publishDate |
2021 |
url |
https://hdl.handle.net/11250/2985449 https://doi.org/10.1016/j.aquaculture.2021.737350 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Aquaculture 546 13 |
op_relation |
Havforskningsinstituttet: 14930-04 EC/H2020/652831 NORCE Norwegian Research Centre AS: AE150010 Aquaculture. 2021, 546 . urn:issn:0044-8486 https://hdl.handle.net/11250/2985449 https://doi.org/10.1016/j.aquaculture.2021.737350 cristin:2002872 |
op_rights |
Navngivelse 4.0 Internasjonal http://creativecommons.org/licenses/by/4.0/deed.no © 2021 The Author(s) |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1016/j.aquaculture.2021.737350 |
container_title |
Aquaculture |
container_volume |
546 |
container_start_page |
737350 |
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1766362483862274048 |