Expression and localization of the aryl hydrocarbon receptors and cytochrome P450 1A during early development of Atlantic cod (Gadus morhua)
The aryl hydrocarbon receptor (Ahr) is a ligand-activated transcription factor that mediates the toxicity of dioxins and dioxin-like compounds (DLCs) in vertebrates. Two clades of the Ahr family exist in teleosts (Ahr1 and Ahr2), and it has been demonstrated that Ahr2 is the main protein involved in...
Published in: | Aquatic Toxicology |
---|---|
Main Authors: | , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2020
|
Subjects: | |
Online Access: | https://hdl.handle.net/11250/2678459 https://doi.org/10.1016/j.aquatox.2020.105558 |
id |
ftnorce:oai:norceresearch.brage.unit.no:11250/2678459 |
---|---|
record_format |
openpolar |
spelling |
ftnorce:oai:norceresearch.brage.unit.no:11250/2678459 2023-05-15T15:26:57+02:00 Expression and localization of the aryl hydrocarbon receptors and cytochrome P450 1A during early development of Atlantic cod (Gadus morhua) Aranguren Abadía, Libe Donald, Carey Eilertsen, Mariann Gharbi, Naouel Tronci, Valentina Sørhus, Elin Mayer, Philipp Nilsen, Tom Ole Meier, Sonnich Goksøyr, Anders Karlsen, Odd Andre 2020 application/pdf https://hdl.handle.net/11250/2678459 https://doi.org/10.1016/j.aquatox.2020.105558 eng eng Norges forskningsråd: 244564 Norges forskningsråd: 254894 Norges forskningsråd: 248840 Norges forskningsråd: 267820 Aquatic Toxicology. 2020, 226 . urn:issn:0166-445X https://hdl.handle.net/11250/2678459 https://doi.org/10.1016/j.aquatox.2020.105558 cristin:1820448 CC BY 4.0 https://creativecommons.org/licenses/by/4.0/ © 2020, Authors CC-BY Aquatic Toxicology 226 10 Peer reviewed Journal article 2020 ftnorce https://doi.org/10.1016/j.aquatox.2020.105558 2022-10-13T05:50:44Z The aryl hydrocarbon receptor (Ahr) is a ligand-activated transcription factor that mediates the toxicity of dioxins and dioxin-like compounds (DLCs) in vertebrates. Two clades of the Ahr family exist in teleosts (Ahr1 and Ahr2), and it has been demonstrated that Ahr2 is the main protein involved in mediating the toxicity of dioxins and DLCs in most teleost species. Recently, we characterized the Atlantic cod (Gadus morhua) Ahr1a and Ahr2a receptors. To further explore a possible subfunction partitioning of Ahr1a and Ahr2a in Atlantic cod we have mapped the expression and localization of ahr1a and ahr2a in early developmental stages. Atlantic cod embryos were continuously exposed in a passive-dosing exposure system to the Ahr agonist, benzo[a]pyrene (B[a]P), from five days post fertilization (dpf) until three days post hatching (dph). Expression of ahr1a, ahr2a, and the Ahr-target genes, cyp1a and ahrrb, was assessed in embryos (8 dpf and 10 dpf) and larvae (3 dph) with quantitative real-time PCR analyses (qPCR), while in situ hybridization was used to assess the localization of expression of ahr1a, ahr2a and cyp1a. Quantitative measurements showed an increased cyp1a expression in B[a]P-exposed samples at all sampling points, and for ahr2a at 10 dpf, confirming the activation of the Ahr-signalling pathway. Furthermore, B[a]P strongly induced ahr2a and cyp1a expression in the cardiovascular system and skin, respectively, of embryos and larvae. Induced expression of both ahr2a and cyp1a was also revealed in the liver of B[a]P-exposed larvae. Our results suggest that Ahr2a is the major subtype involved in mediating responses to B[a]P in early developmental stages of Atlantic cod, which involves transcriptional regulation of biotransformation genes, such as cyp1a. The focused expression of ahr1a in the eye of embryos and larvae, and the presence of ahr2a transcripts in the jaws and fin nodes, further indicate evolved specialized roles of the two Ahrs in ontogenesis. publishedVersion Article in Journal/Newspaper atlantic cod Gadus morhua NORCE vitenarkiv (Norwegian Research Centre) Aquatic Toxicology 226 105558 |
institution |
Open Polar |
collection |
NORCE vitenarkiv (Norwegian Research Centre) |
op_collection_id |
ftnorce |
language |
English |
description |
The aryl hydrocarbon receptor (Ahr) is a ligand-activated transcription factor that mediates the toxicity of dioxins and dioxin-like compounds (DLCs) in vertebrates. Two clades of the Ahr family exist in teleosts (Ahr1 and Ahr2), and it has been demonstrated that Ahr2 is the main protein involved in mediating the toxicity of dioxins and DLCs in most teleost species. Recently, we characterized the Atlantic cod (Gadus morhua) Ahr1a and Ahr2a receptors. To further explore a possible subfunction partitioning of Ahr1a and Ahr2a in Atlantic cod we have mapped the expression and localization of ahr1a and ahr2a in early developmental stages. Atlantic cod embryos were continuously exposed in a passive-dosing exposure system to the Ahr agonist, benzo[a]pyrene (B[a]P), from five days post fertilization (dpf) until three days post hatching (dph). Expression of ahr1a, ahr2a, and the Ahr-target genes, cyp1a and ahrrb, was assessed in embryos (8 dpf and 10 dpf) and larvae (3 dph) with quantitative real-time PCR analyses (qPCR), while in situ hybridization was used to assess the localization of expression of ahr1a, ahr2a and cyp1a. Quantitative measurements showed an increased cyp1a expression in B[a]P-exposed samples at all sampling points, and for ahr2a at 10 dpf, confirming the activation of the Ahr-signalling pathway. Furthermore, B[a]P strongly induced ahr2a and cyp1a expression in the cardiovascular system and skin, respectively, of embryos and larvae. Induced expression of both ahr2a and cyp1a was also revealed in the liver of B[a]P-exposed larvae. Our results suggest that Ahr2a is the major subtype involved in mediating responses to B[a]P in early developmental stages of Atlantic cod, which involves transcriptional regulation of biotransformation genes, such as cyp1a. The focused expression of ahr1a in the eye of embryos and larvae, and the presence of ahr2a transcripts in the jaws and fin nodes, further indicate evolved specialized roles of the two Ahrs in ontogenesis. publishedVersion |
format |
Article in Journal/Newspaper |
author |
Aranguren Abadía, Libe Donald, Carey Eilertsen, Mariann Gharbi, Naouel Tronci, Valentina Sørhus, Elin Mayer, Philipp Nilsen, Tom Ole Meier, Sonnich Goksøyr, Anders Karlsen, Odd Andre |
spellingShingle |
Aranguren Abadía, Libe Donald, Carey Eilertsen, Mariann Gharbi, Naouel Tronci, Valentina Sørhus, Elin Mayer, Philipp Nilsen, Tom Ole Meier, Sonnich Goksøyr, Anders Karlsen, Odd Andre Expression and localization of the aryl hydrocarbon receptors and cytochrome P450 1A during early development of Atlantic cod (Gadus morhua) |
author_facet |
Aranguren Abadía, Libe Donald, Carey Eilertsen, Mariann Gharbi, Naouel Tronci, Valentina Sørhus, Elin Mayer, Philipp Nilsen, Tom Ole Meier, Sonnich Goksøyr, Anders Karlsen, Odd Andre |
author_sort |
Aranguren Abadía, Libe |
title |
Expression and localization of the aryl hydrocarbon receptors and cytochrome P450 1A during early development of Atlantic cod (Gadus morhua) |
title_short |
Expression and localization of the aryl hydrocarbon receptors and cytochrome P450 1A during early development of Atlantic cod (Gadus morhua) |
title_full |
Expression and localization of the aryl hydrocarbon receptors and cytochrome P450 1A during early development of Atlantic cod (Gadus morhua) |
title_fullStr |
Expression and localization of the aryl hydrocarbon receptors and cytochrome P450 1A during early development of Atlantic cod (Gadus morhua) |
title_full_unstemmed |
Expression and localization of the aryl hydrocarbon receptors and cytochrome P450 1A during early development of Atlantic cod (Gadus morhua) |
title_sort |
expression and localization of the aryl hydrocarbon receptors and cytochrome p450 1a during early development of atlantic cod (gadus morhua) |
publishDate |
2020 |
url |
https://hdl.handle.net/11250/2678459 https://doi.org/10.1016/j.aquatox.2020.105558 |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
Aquatic Toxicology 226 10 |
op_relation |
Norges forskningsråd: 244564 Norges forskningsråd: 254894 Norges forskningsråd: 248840 Norges forskningsråd: 267820 Aquatic Toxicology. 2020, 226 . urn:issn:0166-445X https://hdl.handle.net/11250/2678459 https://doi.org/10.1016/j.aquatox.2020.105558 cristin:1820448 |
op_rights |
CC BY 4.0 https://creativecommons.org/licenses/by/4.0/ © 2020, Authors |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1016/j.aquatox.2020.105558 |
container_title |
Aquatic Toxicology |
container_volume |
226 |
container_start_page |
105558 |
_version_ |
1766357409291304960 |