Correlations among carbohydrate inventories, enzyme activities, and microbial communities in the western North Atlantic Ocean
Heterotrophic bacteria process nearly half of the organic matter produced by phytoplankton in the surface ocean. Much of this organic matter consists of high molecular weight (HMW) biopolymers such as polysaccharides and proteins, which must initially be hydrolyzed to smaller sizes by structurally s...
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ftnonlinearchiv:oai:noa.gwlb.de:cop_mods_00072544 2024-04-28T08:30:08+00:00 Correlations among carbohydrate inventories, enzyme activities, and microbial communities in the western North Atlantic Ocean Lloyd, C. Chad Brown, Sarah Giljan, Greta Ghobrial, Sherif Vidal-Melgosa, Silvia Steinke, Nicola Hehemann, Jan-Hendrik Amann, Rudolf Arnosti, Carol 2024-03 electronic https://doi.org/10.5194/egusphere-2024-615 https://noa.gwlb.de/receive/cop_mods_00072544 https://noa.gwlb.de/servlets/MCRFileNodeServlet/cop_derivate_00070751/egusphere-2024-615.pdf https://egusphere.copernicus.org/preprints/2024/egusphere-2024-615/egusphere-2024-615.pdf eng eng Copernicus Publications https://doi.org/10.5194/egusphere-2024-615 https://noa.gwlb.de/receive/cop_mods_00072544 https://noa.gwlb.de/servlets/MCRFileNodeServlet/cop_derivate_00070751/egusphere-2024-615.pdf https://egusphere.copernicus.org/preprints/2024/egusphere-2024-615/egusphere-2024-615.pdf https://creativecommons.org/licenses/by/4.0/ uneingeschränkt info:eu-repo/semantics/openAccess article Verlagsveröffentlichung article Text doc-type:article 2024 ftnonlinearchiv https://doi.org/10.5194/egusphere-2024-615 2024-04-02T16:51:04Z Heterotrophic bacteria process nearly half of the organic matter produced by phytoplankton in the surface ocean. Much of this organic matter consists of high molecular weight (HMW) biopolymers such as polysaccharides and proteins, which must initially be hydrolyzed to smaller sizes by structurally specific extracellular enzymes. To assess the relationships between substrate structure and microbial community composition and function, we concurrently determined carbohydrate abundance and structural complexity, bacterial community composition, and peptidase and polysaccharide hydrolase activities throughout the water column at four distinct stations in the western North Atlantic Ocean. Although the monosaccharide constituents of particulate organic matter (POM) were similar among stations, the structural complexity of POM-derived polysaccharides varied by depth and station, as demonstrated by polysaccharide-specific antibody probing. Bacterial community composition and polysaccharide hydrolase activities also varied by depth and station, suggesting that the structure and function of bacterial communities—and the structural complexity of their target substrates—are interlinked. Thus, the extent to which bacteria can transform organic matter in the ocean is dependent on both the structural complexity of the organic matter and their enzymatic capabilities in different depths and regions of the ocean. Article in Journal/Newspaper North Atlantic Niedersächsisches Online-Archiv NOA |
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English |
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article Verlagsveröffentlichung |
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article Verlagsveröffentlichung Lloyd, C. Chad Brown, Sarah Giljan, Greta Ghobrial, Sherif Vidal-Melgosa, Silvia Steinke, Nicola Hehemann, Jan-Hendrik Amann, Rudolf Arnosti, Carol Correlations among carbohydrate inventories, enzyme activities, and microbial communities in the western North Atlantic Ocean |
topic_facet |
article Verlagsveröffentlichung |
description |
Heterotrophic bacteria process nearly half of the organic matter produced by phytoplankton in the surface ocean. Much of this organic matter consists of high molecular weight (HMW) biopolymers such as polysaccharides and proteins, which must initially be hydrolyzed to smaller sizes by structurally specific extracellular enzymes. To assess the relationships between substrate structure and microbial community composition and function, we concurrently determined carbohydrate abundance and structural complexity, bacterial community composition, and peptidase and polysaccharide hydrolase activities throughout the water column at four distinct stations in the western North Atlantic Ocean. Although the monosaccharide constituents of particulate organic matter (POM) were similar among stations, the structural complexity of POM-derived polysaccharides varied by depth and station, as demonstrated by polysaccharide-specific antibody probing. Bacterial community composition and polysaccharide hydrolase activities also varied by depth and station, suggesting that the structure and function of bacterial communities—and the structural complexity of their target substrates—are interlinked. Thus, the extent to which bacteria can transform organic matter in the ocean is dependent on both the structural complexity of the organic matter and their enzymatic capabilities in different depths and regions of the ocean. |
format |
Article in Journal/Newspaper |
author |
Lloyd, C. Chad Brown, Sarah Giljan, Greta Ghobrial, Sherif Vidal-Melgosa, Silvia Steinke, Nicola Hehemann, Jan-Hendrik Amann, Rudolf Arnosti, Carol |
author_facet |
Lloyd, C. Chad Brown, Sarah Giljan, Greta Ghobrial, Sherif Vidal-Melgosa, Silvia Steinke, Nicola Hehemann, Jan-Hendrik Amann, Rudolf Arnosti, Carol |
author_sort |
Lloyd, C. Chad |
title |
Correlations among carbohydrate inventories, enzyme activities, and microbial communities in the western North Atlantic Ocean |
title_short |
Correlations among carbohydrate inventories, enzyme activities, and microbial communities in the western North Atlantic Ocean |
title_full |
Correlations among carbohydrate inventories, enzyme activities, and microbial communities in the western North Atlantic Ocean |
title_fullStr |
Correlations among carbohydrate inventories, enzyme activities, and microbial communities in the western North Atlantic Ocean |
title_full_unstemmed |
Correlations among carbohydrate inventories, enzyme activities, and microbial communities in the western North Atlantic Ocean |
title_sort |
correlations among carbohydrate inventories, enzyme activities, and microbial communities in the western north atlantic ocean |
publisher |
Copernicus Publications |
publishDate |
2024 |
url |
https://doi.org/10.5194/egusphere-2024-615 https://noa.gwlb.de/receive/cop_mods_00072544 https://noa.gwlb.de/servlets/MCRFileNodeServlet/cop_derivate_00070751/egusphere-2024-615.pdf https://egusphere.copernicus.org/preprints/2024/egusphere-2024-615/egusphere-2024-615.pdf |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_relation |
https://doi.org/10.5194/egusphere-2024-615 https://noa.gwlb.de/receive/cop_mods_00072544 https://noa.gwlb.de/servlets/MCRFileNodeServlet/cop_derivate_00070751/egusphere-2024-615.pdf https://egusphere.copernicus.org/preprints/2024/egusphere-2024-615/egusphere-2024-615.pdf |
op_rights |
https://creativecommons.org/licenses/by/4.0/ uneingeschränkt info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.5194/egusphere-2024-615 |
_version_ |
1797588106803675136 |