Microscopic and spectroscopic investigations of uranium(VI) reduction by Desulfosporosinus hippei DSM 8344
Clay formations are potential host rocks for the long-term storage of high-level radioactive waste in a deep geological repository. Bentonites are supposed to serve as backfill material, not only for a final disposal site in clay formations but also in crystalline rock. For a long-term safety assess...
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2021
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ftnonlinearchiv:oai:noa.gwlb.de:cop_mods_00058731 2024-09-15T18:30:12+00:00 Microscopic and spectroscopic investigations of uranium(VI) reduction by Desulfosporosinus hippei DSM 8344 Hilpmann, Stephan Steudtner, Robin Drobot, Björn Hübner, René Bok, Frank Stumpf, Thorsten Cherkouk, Andrea 2021-11 electronic https://doi.org/10.5194/sand-1-155-2021 https://noa.gwlb.de/receive/cop_mods_00058731 https://noa.gwlb.de/servlets/MCRFileNodeServlet/cop_derivate_00058355/sand-1-155-2021.pdf https://sand.copernicus.org/articles/1/155/2021/sand-1-155-2021.pdf eng eng Copernicus Publications Safety of nuclear waste disposal -- https://www.safety-of-nuclear-waste-disposal.net/ -- https://sand.copernicus.org/articles/1/index.html -- 2749-4802 https://doi.org/10.5194/sand-1-155-2021 https://noa.gwlb.de/receive/cop_mods_00058731 https://noa.gwlb.de/servlets/MCRFileNodeServlet/cop_derivate_00058355/sand-1-155-2021.pdf https://sand.copernicus.org/articles/1/155/2021/sand-1-155-2021.pdf https://creativecommons.org/licenses/by/4.0/ uneingeschränkt info:eu-repo/semantics/openAccess article Verlagsveröffentlichung article Text doc-type:article 2021 ftnonlinearchiv https://doi.org/10.5194/sand-1-155-2021 2024-06-26T04:36:34Z Clay formations are potential host rocks for the long-term storage of high-level radioactive waste in a deep geological repository. Bentonites are supposed to serve as backfill material, not only for a final disposal site in clay formations but also in crystalline rock. For a long-term safety assessment, various aspects must be taken into account. Besides geological, geochemical and geophysical considerations, naturally occurring microorganisms also play a crucial part in the environment of such a repository. In the event of a worst-case scenario when water enters the disposal site, they can interact with the radionuclides and change for example the chemical speciation or the oxidation state (Lloyd et al., 2002). Desulfosporosinus spp. are an important representative of anaerobic, sulfate-reducing microorganisms, which are present in clay formations as well as in bentonites. Various studies have shown that they play a major role in the microbial communities of these surroundings (Bagnoud et al., 2016; Matschiavelli et al., 2019). A closely related microorganism to the isolated species is Desulfosporosinus hippei DSM 8344, which was originally found in permafrost soil (Vatsurina et al., 2008). This bacterium was used to investigate its interactions with uranium(VI) especially regarding the reduction to the less mobile uranium(IV). Time-dependent reduction experiments in artificial Opalinus Clay pore water (Wersin et al., 2011) (100 µM uranium(VI), pH 5.5) showed the removal of about 80 % of the uranium(VI) from the supernatants within 48 h. Corresponding UV/Vis measurements of the dissolved cell pellets exhibited an increasing proportion of uranium(IV) in the cell-bound uranium. Calculations with the inclusion of extinction coefficients led to a ratio of 39 % uranium(IV) after 1 week. Therefore, a combined sorption-reduction process is a possible interaction mechanism. Time-resolved laser-induced luminescence spectroscopy verified the presence of two uranium(VI) species in the supernatant. A comparison with ... Article in Journal/Newspaper permafrost Niedersächsisches Online-Archiv NOA Safety of Nuclear Waste Disposal 1 155 156 |
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article Verlagsveröffentlichung Hilpmann, Stephan Steudtner, Robin Drobot, Björn Hübner, René Bok, Frank Stumpf, Thorsten Cherkouk, Andrea Microscopic and spectroscopic investigations of uranium(VI) reduction by Desulfosporosinus hippei DSM 8344 |
topic_facet |
article Verlagsveröffentlichung |
description |
Clay formations are potential host rocks for the long-term storage of high-level radioactive waste in a deep geological repository. Bentonites are supposed to serve as backfill material, not only for a final disposal site in clay formations but also in crystalline rock. For a long-term safety assessment, various aspects must be taken into account. Besides geological, geochemical and geophysical considerations, naturally occurring microorganisms also play a crucial part in the environment of such a repository. In the event of a worst-case scenario when water enters the disposal site, they can interact with the radionuclides and change for example the chemical speciation or the oxidation state (Lloyd et al., 2002). Desulfosporosinus spp. are an important representative of anaerobic, sulfate-reducing microorganisms, which are present in clay formations as well as in bentonites. Various studies have shown that they play a major role in the microbial communities of these surroundings (Bagnoud et al., 2016; Matschiavelli et al., 2019). A closely related microorganism to the isolated species is Desulfosporosinus hippei DSM 8344, which was originally found in permafrost soil (Vatsurina et al., 2008). This bacterium was used to investigate its interactions with uranium(VI) especially regarding the reduction to the less mobile uranium(IV). Time-dependent reduction experiments in artificial Opalinus Clay pore water (Wersin et al., 2011) (100 µM uranium(VI), pH 5.5) showed the removal of about 80 % of the uranium(VI) from the supernatants within 48 h. Corresponding UV/Vis measurements of the dissolved cell pellets exhibited an increasing proportion of uranium(IV) in the cell-bound uranium. Calculations with the inclusion of extinction coefficients led to a ratio of 39 % uranium(IV) after 1 week. Therefore, a combined sorption-reduction process is a possible interaction mechanism. Time-resolved laser-induced luminescence spectroscopy verified the presence of two uranium(VI) species in the supernatant. A comparison with ... |
format |
Article in Journal/Newspaper |
author |
Hilpmann, Stephan Steudtner, Robin Drobot, Björn Hübner, René Bok, Frank Stumpf, Thorsten Cherkouk, Andrea |
author_facet |
Hilpmann, Stephan Steudtner, Robin Drobot, Björn Hübner, René Bok, Frank Stumpf, Thorsten Cherkouk, Andrea |
author_sort |
Hilpmann, Stephan |
title |
Microscopic and spectroscopic investigations of uranium(VI) reduction by Desulfosporosinus hippei DSM 8344 |
title_short |
Microscopic and spectroscopic investigations of uranium(VI) reduction by Desulfosporosinus hippei DSM 8344 |
title_full |
Microscopic and spectroscopic investigations of uranium(VI) reduction by Desulfosporosinus hippei DSM 8344 |
title_fullStr |
Microscopic and spectroscopic investigations of uranium(VI) reduction by Desulfosporosinus hippei DSM 8344 |
title_full_unstemmed |
Microscopic and spectroscopic investigations of uranium(VI) reduction by Desulfosporosinus hippei DSM 8344 |
title_sort |
microscopic and spectroscopic investigations of uranium(vi) reduction by desulfosporosinus hippei dsm 8344 |
publisher |
Copernicus Publications |
publishDate |
2021 |
url |
https://doi.org/10.5194/sand-1-155-2021 https://noa.gwlb.de/receive/cop_mods_00058731 https://noa.gwlb.de/servlets/MCRFileNodeServlet/cop_derivate_00058355/sand-1-155-2021.pdf https://sand.copernicus.org/articles/1/155/2021/sand-1-155-2021.pdf |
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permafrost |
genre_facet |
permafrost |
op_relation |
Safety of nuclear waste disposal -- https://www.safety-of-nuclear-waste-disposal.net/ -- https://sand.copernicus.org/articles/1/index.html -- 2749-4802 https://doi.org/10.5194/sand-1-155-2021 https://noa.gwlb.de/receive/cop_mods_00058731 https://noa.gwlb.de/servlets/MCRFileNodeServlet/cop_derivate_00058355/sand-1-155-2021.pdf https://sand.copernicus.org/articles/1/155/2021/sand-1-155-2021.pdf |
op_rights |
https://creativecommons.org/licenses/by/4.0/ uneingeschränkt info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.5194/sand-1-155-2021 |
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Safety of Nuclear Waste Disposal |
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1 |
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155 |
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156 |
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1810471663496593408 |