Spatial variability of particle-attached and free-living bacterial diversity in surface waters from the Mackenzie River to the Beaufort Sea (Canadian Arctic)
We explored the patterns of total and active bacterial community structure in a gradient covering surface waters from the Mackenzie River to the coastal Beaufort Sea in the Canadian Arctic Ocean, with a particular focus on free-living (FL) vs. particle-attached (PA) communities. Capillary electropho...
Published in: | Biogeosciences |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Copernicus Publications
2013
|
Subjects: | |
Online Access: | https://doi.org/10.5194/bg-10-2747-2013 https://noa.gwlb.de/receive/cop_mods_00022942 https://noa.gwlb.de/servlets/MCRFileNodeServlet/cop_derivate_00022897/bg-10-2747-2013.pdf https://bg.copernicus.org/articles/10/2747/2013/bg-10-2747-2013.pdf |
id |
ftnonlinearchiv:oai:noa.gwlb.de:cop_mods_00022942 |
---|---|
record_format |
openpolar |
spelling |
ftnonlinearchiv:oai:noa.gwlb.de:cop_mods_00022942 2023-05-15T15:01:54+02:00 Spatial variability of particle-attached and free-living bacterial diversity in surface waters from the Mackenzie River to the Beaufort Sea (Canadian Arctic) Ortega-Retuerta, E. Joux, F. Jeffrey, W. H. Ghiglione, J. F. 2013-04 electronic https://doi.org/10.5194/bg-10-2747-2013 https://noa.gwlb.de/receive/cop_mods_00022942 https://noa.gwlb.de/servlets/MCRFileNodeServlet/cop_derivate_00022897/bg-10-2747-2013.pdf https://bg.copernicus.org/articles/10/2747/2013/bg-10-2747-2013.pdf eng eng Copernicus Publications Biogeosciences -- http://www.bibliothek.uni-regensburg.de/ezeit/?2158181 -- http://www.copernicus.org/EGU/bg/bg.html -- 1726-4189 https://doi.org/10.5194/bg-10-2747-2013 https://noa.gwlb.de/receive/cop_mods_00022942 https://noa.gwlb.de/servlets/MCRFileNodeServlet/cop_derivate_00022897/bg-10-2747-2013.pdf https://bg.copernicus.org/articles/10/2747/2013/bg-10-2747-2013.pdf uneingeschränkt info:eu-repo/semantics/openAccess article Verlagsveröffentlichung article Text doc-type:article 2013 ftnonlinearchiv https://doi.org/10.5194/bg-10-2747-2013 2022-02-08T22:50:53Z We explored the patterns of total and active bacterial community structure in a gradient covering surface waters from the Mackenzie River to the coastal Beaufort Sea in the Canadian Arctic Ocean, with a particular focus on free-living (FL) vs. particle-attached (PA) communities. Capillary electrophoresis–single-strand conformation polymorphism (CE-SSCP) showed significant differences when comparing river, coast and open sea bacterial community structures. In contrast to the river and coastal waters, total (16S rDNA-based) and active (16S rRNA-based) communities in the open sea samples were not significantly different, suggesting that most present bacterial groups were equally active in this area. Additionally, we observed significant differences between PA and FL bacterial community structure in the open sea, but similar structure in the two fractions for coastal and river samples. Direct multivariate statistical analyses showed that total community structure was mainly driven by salinity (a proxy of dissolved organic carbon and chromophoric dissolved organic matter), suspended particles, amino acids and chlorophyll a. Pyrosequencing of 16S rRNA genes from selected samples confirmed significant differences between river, coastal and sea samples. The PA fraction was only different (15.7% similarity) from the FL one in the open sea sample. Furthermore, PA samples generally showed higher diversity (Shannon, Simpson and Chao indices) than FL samples. At the class level, Opitutae was most abundant in the PA fraction of the sea sample, followed by Flavobacteria and Gammaproteobacteria, while the FL sea sample was dominated by Alphaproteobacteria. Finally, for the coast and river samples and both PA and FL fractions, Betaproteobacteria, Alphaproteobacteria and Actinobacteria were dominant. These results highlight the coexistence of particle specialists and generalists and the role of particle quality in structuring bacterial communities in the area. These results may also serve as a basis to predict further changes in bacterial communities should climate change lead to further increases in river discharge and related particle loads. Article in Journal/Newspaper Arctic Arctic Ocean Beaufort Sea Climate change Mackenzie river Niedersächsisches Online-Archiv NOA Arctic Arctic Ocean Mackenzie River Biogeosciences 10 4 2747 2759 |
institution |
Open Polar |
collection |
Niedersächsisches Online-Archiv NOA |
op_collection_id |
ftnonlinearchiv |
language |
English |
topic |
article Verlagsveröffentlichung |
spellingShingle |
article Verlagsveröffentlichung Ortega-Retuerta, E. Joux, F. Jeffrey, W. H. Ghiglione, J. F. Spatial variability of particle-attached and free-living bacterial diversity in surface waters from the Mackenzie River to the Beaufort Sea (Canadian Arctic) |
topic_facet |
article Verlagsveröffentlichung |
description |
We explored the patterns of total and active bacterial community structure in a gradient covering surface waters from the Mackenzie River to the coastal Beaufort Sea in the Canadian Arctic Ocean, with a particular focus on free-living (FL) vs. particle-attached (PA) communities. Capillary electrophoresis–single-strand conformation polymorphism (CE-SSCP) showed significant differences when comparing river, coast and open sea bacterial community structures. In contrast to the river and coastal waters, total (16S rDNA-based) and active (16S rRNA-based) communities in the open sea samples were not significantly different, suggesting that most present bacterial groups were equally active in this area. Additionally, we observed significant differences between PA and FL bacterial community structure in the open sea, but similar structure in the two fractions for coastal and river samples. Direct multivariate statistical analyses showed that total community structure was mainly driven by salinity (a proxy of dissolved organic carbon and chromophoric dissolved organic matter), suspended particles, amino acids and chlorophyll a. Pyrosequencing of 16S rRNA genes from selected samples confirmed significant differences between river, coastal and sea samples. The PA fraction was only different (15.7% similarity) from the FL one in the open sea sample. Furthermore, PA samples generally showed higher diversity (Shannon, Simpson and Chao indices) than FL samples. At the class level, Opitutae was most abundant in the PA fraction of the sea sample, followed by Flavobacteria and Gammaproteobacteria, while the FL sea sample was dominated by Alphaproteobacteria. Finally, for the coast and river samples and both PA and FL fractions, Betaproteobacteria, Alphaproteobacteria and Actinobacteria were dominant. These results highlight the coexistence of particle specialists and generalists and the role of particle quality in structuring bacterial communities in the area. These results may also serve as a basis to predict further changes in bacterial communities should climate change lead to further increases in river discharge and related particle loads. |
format |
Article in Journal/Newspaper |
author |
Ortega-Retuerta, E. Joux, F. Jeffrey, W. H. Ghiglione, J. F. |
author_facet |
Ortega-Retuerta, E. Joux, F. Jeffrey, W. H. Ghiglione, J. F. |
author_sort |
Ortega-Retuerta, E. |
title |
Spatial variability of particle-attached and free-living bacterial diversity in surface waters from the Mackenzie River to the Beaufort Sea (Canadian Arctic) |
title_short |
Spatial variability of particle-attached and free-living bacterial diversity in surface waters from the Mackenzie River to the Beaufort Sea (Canadian Arctic) |
title_full |
Spatial variability of particle-attached and free-living bacterial diversity in surface waters from the Mackenzie River to the Beaufort Sea (Canadian Arctic) |
title_fullStr |
Spatial variability of particle-attached and free-living bacterial diversity in surface waters from the Mackenzie River to the Beaufort Sea (Canadian Arctic) |
title_full_unstemmed |
Spatial variability of particle-attached and free-living bacterial diversity in surface waters from the Mackenzie River to the Beaufort Sea (Canadian Arctic) |
title_sort |
spatial variability of particle-attached and free-living bacterial diversity in surface waters from the mackenzie river to the beaufort sea (canadian arctic) |
publisher |
Copernicus Publications |
publishDate |
2013 |
url |
https://doi.org/10.5194/bg-10-2747-2013 https://noa.gwlb.de/receive/cop_mods_00022942 https://noa.gwlb.de/servlets/MCRFileNodeServlet/cop_derivate_00022897/bg-10-2747-2013.pdf https://bg.copernicus.org/articles/10/2747/2013/bg-10-2747-2013.pdf |
geographic |
Arctic Arctic Ocean Mackenzie River |
geographic_facet |
Arctic Arctic Ocean Mackenzie River |
genre |
Arctic Arctic Ocean Beaufort Sea Climate change Mackenzie river |
genre_facet |
Arctic Arctic Ocean Beaufort Sea Climate change Mackenzie river |
op_relation |
Biogeosciences -- http://www.bibliothek.uni-regensburg.de/ezeit/?2158181 -- http://www.copernicus.org/EGU/bg/bg.html -- 1726-4189 https://doi.org/10.5194/bg-10-2747-2013 https://noa.gwlb.de/receive/cop_mods_00022942 https://noa.gwlb.de/servlets/MCRFileNodeServlet/cop_derivate_00022897/bg-10-2747-2013.pdf https://bg.copernicus.org/articles/10/2747/2013/bg-10-2747-2013.pdf |
op_rights |
uneingeschränkt info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.5194/bg-10-2747-2013 |
container_title |
Biogeosciences |
container_volume |
10 |
container_issue |
4 |
container_start_page |
2747 |
op_container_end_page |
2759 |
_version_ |
1766333912771985408 |