Technical Note: Precise quantitative measurements of total dissolved inorganic carbon from small amounts of seawater using a gas chromatographic system
Total dissolved inorganic carbon (CT) is one of the most frequently measured parameters used to calculate the partial pressure of carbon dioxide in seawater. Its determination has become increasingly important because of the rising interest in the biological effects of ocean acidification. Coulometr...
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Copernicus Publications
2013
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ftnonlinearchiv:oai:noa.gwlb.de:cop_mods_00021349 2023-05-15T17:51:45+02:00 Technical Note: Precise quantitative measurements of total dissolved inorganic carbon from small amounts of seawater using a gas chromatographic system Hansen, T. Gardeler, B. Matthiessen, B. 2013-10 electronic https://doi.org/10.5194/bg-10-6601-2013 https://noa.gwlb.de/receive/cop_mods_00021349 https://noa.gwlb.de/servlets/MCRFileNodeServlet/cop_derivate_00021304/bg-10-6601-2013.pdf https://bg.copernicus.org/articles/10/6601/2013/bg-10-6601-2013.pdf eng eng Copernicus Publications Biogeosciences -- http://www.bibliothek.uni-regensburg.de/ezeit/?2158181 -- http://www.copernicus.org/EGU/bg/bg.html -- 1726-4189 https://doi.org/10.5194/bg-10-6601-2013 https://noa.gwlb.de/receive/cop_mods_00021349 https://noa.gwlb.de/servlets/MCRFileNodeServlet/cop_derivate_00021304/bg-10-6601-2013.pdf https://bg.copernicus.org/articles/10/6601/2013/bg-10-6601-2013.pdf uneingeschränkt info:eu-repo/semantics/openAccess article Verlagsveröffentlichung article Text doc-type:article 2013 ftnonlinearchiv https://doi.org/10.5194/bg-10-6601-2013 2022-02-08T22:51:42Z Total dissolved inorganic carbon (CT) is one of the most frequently measured parameters used to calculate the partial pressure of carbon dioxide in seawater. Its determination has become increasingly important because of the rising interest in the biological effects of ocean acidification. Coulometric and infrared detection methods are currently favored in order to precisely quantify CT. These methods however are not sufficiently validated for CT measurements of biological experiments manipulating seawater carbonate chemistry with an extended CT measurement range (~1250–2400 μmol kg–1) compared to natural open ocean seawater (~1950–2200 μmol kg−1). The requirement of total sample amounts between 0.1–1 L seawater in the coulometric- and infrared detection methods potentially exclude their use for experiments working with much smaller volumes. Additionally, precise CT analytics become difficult with high amounts of biomass (e.g., phytoplankton cultures) or even impossible in the presence of planktonic calcifiers without sample pre-filtration. Filtration however, can alter CT concentration through gas exchange induced by high pressure. Addressing these problems, we present precise quantification of CT using a small, basic and inexpensive gas chromatograph as a CT analyzer. Our technique is able to provide a repeatability of ±3.1 μmol kg−1, given by the pooled standard deviation over a CT range typically applied in acidification experiments. 200 μL of sample is required to perform the actual CT measurement. The total sample amount needed is 12 mL. Moreover, we show that sample filtration is applicable with only minor alteration of the CT. The method is simple, reliable and with low cumulative material costs. Hence, it is potentially attractive for all researchers experimentally manipulating the seawater carbonate system. Article in Journal/Newspaper Ocean acidification Niedersächsisches Online-Archiv NOA Biogeosciences 10 10 6601 6608 |
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article Verlagsveröffentlichung Hansen, T. Gardeler, B. Matthiessen, B. Technical Note: Precise quantitative measurements of total dissolved inorganic carbon from small amounts of seawater using a gas chromatographic system |
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article Verlagsveröffentlichung |
description |
Total dissolved inorganic carbon (CT) is one of the most frequently measured parameters used to calculate the partial pressure of carbon dioxide in seawater. Its determination has become increasingly important because of the rising interest in the biological effects of ocean acidification. Coulometric and infrared detection methods are currently favored in order to precisely quantify CT. These methods however are not sufficiently validated for CT measurements of biological experiments manipulating seawater carbonate chemistry with an extended CT measurement range (~1250–2400 μmol kg–1) compared to natural open ocean seawater (~1950–2200 μmol kg−1). The requirement of total sample amounts between 0.1–1 L seawater in the coulometric- and infrared detection methods potentially exclude their use for experiments working with much smaller volumes. Additionally, precise CT analytics become difficult with high amounts of biomass (e.g., phytoplankton cultures) or even impossible in the presence of planktonic calcifiers without sample pre-filtration. Filtration however, can alter CT concentration through gas exchange induced by high pressure. Addressing these problems, we present precise quantification of CT using a small, basic and inexpensive gas chromatograph as a CT analyzer. Our technique is able to provide a repeatability of ±3.1 μmol kg−1, given by the pooled standard deviation over a CT range typically applied in acidification experiments. 200 μL of sample is required to perform the actual CT measurement. The total sample amount needed is 12 mL. Moreover, we show that sample filtration is applicable with only minor alteration of the CT. The method is simple, reliable and with low cumulative material costs. Hence, it is potentially attractive for all researchers experimentally manipulating the seawater carbonate system. |
format |
Article in Journal/Newspaper |
author |
Hansen, T. Gardeler, B. Matthiessen, B. |
author_facet |
Hansen, T. Gardeler, B. Matthiessen, B. |
author_sort |
Hansen, T. |
title |
Technical Note: Precise quantitative measurements of total dissolved inorganic carbon from small amounts of seawater using a gas chromatographic system |
title_short |
Technical Note: Precise quantitative measurements of total dissolved inorganic carbon from small amounts of seawater using a gas chromatographic system |
title_full |
Technical Note: Precise quantitative measurements of total dissolved inorganic carbon from small amounts of seawater using a gas chromatographic system |
title_fullStr |
Technical Note: Precise quantitative measurements of total dissolved inorganic carbon from small amounts of seawater using a gas chromatographic system |
title_full_unstemmed |
Technical Note: Precise quantitative measurements of total dissolved inorganic carbon from small amounts of seawater using a gas chromatographic system |
title_sort |
technical note: precise quantitative measurements of total dissolved inorganic carbon from small amounts of seawater using a gas chromatographic system |
publisher |
Copernicus Publications |
publishDate |
2013 |
url |
https://doi.org/10.5194/bg-10-6601-2013 https://noa.gwlb.de/receive/cop_mods_00021349 https://noa.gwlb.de/servlets/MCRFileNodeServlet/cop_derivate_00021304/bg-10-6601-2013.pdf https://bg.copernicus.org/articles/10/6601/2013/bg-10-6601-2013.pdf |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
Biogeosciences -- http://www.bibliothek.uni-regensburg.de/ezeit/?2158181 -- http://www.copernicus.org/EGU/bg/bg.html -- 1726-4189 https://doi.org/10.5194/bg-10-6601-2013 https://noa.gwlb.de/receive/cop_mods_00021349 https://noa.gwlb.de/servlets/MCRFileNodeServlet/cop_derivate_00021304/bg-10-6601-2013.pdf https://bg.copernicus.org/articles/10/6601/2013/bg-10-6601-2013.pdf |
op_rights |
uneingeschränkt info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.5194/bg-10-6601-2013 |
container_title |
Biogeosciences |
container_volume |
10 |
container_issue |
10 |
container_start_page |
6601 |
op_container_end_page |
6608 |
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1766159001112805376 |