Assessing approaches to determine the effect of ocean acidification on bacterial processes
Bacterial extracellular enzymes play a significant role in the degradation of labile organic matter and nutrient availability in the open ocean. Although bacterial production and extracellular enzymes may be affected by ocean acidification, few studies to date have considered the methodology used to...
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2016
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ftnonlinearchiv:oai:noa.gwlb.de:cop_mods_00011714 2023-05-15T17:49:25+02:00 Assessing approaches to determine the effect of ocean acidification on bacterial processes Burrell, Timothy J. Maas, Elizabeth W. Teesdale-Spittle, Paul Law, Cliff S. 2016-08 electronic https://doi.org/10.5194/bg-13-4379-2016 https://noa.gwlb.de/receive/cop_mods_00011714 https://noa.gwlb.de/servlets/MCRFileNodeServlet/cop_derivate_00011671/bg-13-4379-2016.pdf https://bg.copernicus.org/articles/13/4379/2016/bg-13-4379-2016.pdf eng eng Copernicus Publications Biogeosciences -- http://www.bibliothek.uni-regensburg.de/ezeit/?2158181 -- http://www.copernicus.org/EGU/bg/bg.html -- 1726-4189 https://doi.org/10.5194/bg-13-4379-2016 https://noa.gwlb.de/receive/cop_mods_00011714 https://noa.gwlb.de/servlets/MCRFileNodeServlet/cop_derivate_00011671/bg-13-4379-2016.pdf https://bg.copernicus.org/articles/13/4379/2016/bg-13-4379-2016.pdf uneingeschränkt info:eu-repo/semantics/openAccess article Verlagsveröffentlichung article Text doc-type:article 2016 ftnonlinearchiv https://doi.org/10.5194/bg-13-4379-2016 2022-02-08T22:56:23Z Bacterial extracellular enzymes play a significant role in the degradation of labile organic matter and nutrient availability in the open ocean. Although bacterial production and extracellular enzymes may be affected by ocean acidification, few studies to date have considered the methodology used to measure enzyme activity and bacterial processes. This study investigated the potential artefacts in determining the response of bacterial growth and extracellular glucosidase and aminopeptidase activity to ocean acidification as well as the relative effects of three different acidification techniques. Tests confirmed that the observed effect of pH on fluorescence of artificial fluorophores, and the influence of the MCA fluorescent substrate on seawater sample pH, were both overcome by the use of Tris buffer. In experiments testing different acidification methods, bubbling with CO2 gas mixtures resulted in higher β-glucosidase activity and 15–40 % higher bacterial abundance, relative to acidification via gas-permeable silicon tubing and acid addition (HCl). Bubbling may stimulate carbohydrate degradation and bacterial growth, leading to the incorrect interpretation of the impacts of ocean acidification on organic matter cycling. Article in Journal/Newspaper Ocean acidification Niedersächsisches Online-Archiv NOA Biogeosciences 13 15 4379 4388 |
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English |
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article Verlagsveröffentlichung |
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article Verlagsveröffentlichung Burrell, Timothy J. Maas, Elizabeth W. Teesdale-Spittle, Paul Law, Cliff S. Assessing approaches to determine the effect of ocean acidification on bacterial processes |
topic_facet |
article Verlagsveröffentlichung |
description |
Bacterial extracellular enzymes play a significant role in the degradation of labile organic matter and nutrient availability in the open ocean. Although bacterial production and extracellular enzymes may be affected by ocean acidification, few studies to date have considered the methodology used to measure enzyme activity and bacterial processes. This study investigated the potential artefacts in determining the response of bacterial growth and extracellular glucosidase and aminopeptidase activity to ocean acidification as well as the relative effects of three different acidification techniques. Tests confirmed that the observed effect of pH on fluorescence of artificial fluorophores, and the influence of the MCA fluorescent substrate on seawater sample pH, were both overcome by the use of Tris buffer. In experiments testing different acidification methods, bubbling with CO2 gas mixtures resulted in higher β-glucosidase activity and 15–40 % higher bacterial abundance, relative to acidification via gas-permeable silicon tubing and acid addition (HCl). Bubbling may stimulate carbohydrate degradation and bacterial growth, leading to the incorrect interpretation of the impacts of ocean acidification on organic matter cycling. |
format |
Article in Journal/Newspaper |
author |
Burrell, Timothy J. Maas, Elizabeth W. Teesdale-Spittle, Paul Law, Cliff S. |
author_facet |
Burrell, Timothy J. Maas, Elizabeth W. Teesdale-Spittle, Paul Law, Cliff S. |
author_sort |
Burrell, Timothy J. |
title |
Assessing approaches to determine the effect of ocean acidification on bacterial processes |
title_short |
Assessing approaches to determine the effect of ocean acidification on bacterial processes |
title_full |
Assessing approaches to determine the effect of ocean acidification on bacterial processes |
title_fullStr |
Assessing approaches to determine the effect of ocean acidification on bacterial processes |
title_full_unstemmed |
Assessing approaches to determine the effect of ocean acidification on bacterial processes |
title_sort |
assessing approaches to determine the effect of ocean acidification on bacterial processes |
publisher |
Copernicus Publications |
publishDate |
2016 |
url |
https://doi.org/10.5194/bg-13-4379-2016 https://noa.gwlb.de/receive/cop_mods_00011714 https://noa.gwlb.de/servlets/MCRFileNodeServlet/cop_derivate_00011671/bg-13-4379-2016.pdf https://bg.copernicus.org/articles/13/4379/2016/bg-13-4379-2016.pdf |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
Biogeosciences -- http://www.bibliothek.uni-regensburg.de/ezeit/?2158181 -- http://www.copernicus.org/EGU/bg/bg.html -- 1726-4189 https://doi.org/10.5194/bg-13-4379-2016 https://noa.gwlb.de/receive/cop_mods_00011714 https://noa.gwlb.de/servlets/MCRFileNodeServlet/cop_derivate_00011671/bg-13-4379-2016.pdf https://bg.copernicus.org/articles/13/4379/2016/bg-13-4379-2016.pdf |
op_rights |
uneingeschränkt info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.5194/bg-13-4379-2016 |
container_title |
Biogeosciences |
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13 |
container_issue |
15 |
container_start_page |
4379 |
op_container_end_page |
4388 |
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1766155739732115456 |