Calanus finmarchicus as a novel source of health-promoting bioactive peptides: Enzymatic protein hydrolysis, characterization, and in vitro bioactivity
Calanus finmarchicus is a crustacean currently used as a source of marine lipid. The lipids are extracted by enzymatic protein hydrolysis, while the remaining peptide fraction is regarded as a byproduct. In the present work, ten different commercial proteases and endogenous C. finmarchicus proteases...
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ftnofima:oai:nofima.brage.unit.no:11250/3087863 2023-10-09T21:50:28+02:00 Calanus finmarchicus as a novel source of health-promoting bioactive peptides: Enzymatic protein hydrolysis, characterization, and in vitro bioactivity Matic, Josipa Bøgwald, Isak Tengstrand, Erik Rønning, Sissel Beate Afseth, Nils Kristian Wubshet, Sileshi Gizachew 2023 application/pdf https://hdl.handle.net/11250/3087863 https://doi.org/10.1016/j.bcab.2023.102820 eng eng Nofima AS: 202101 Nofima AS: 202102 Norges forskningsråd: 314599 Norges forskningsråd: 314111 Norges forskningsråd: 320086 Nofima AS: 13411 Biocatalysis and Agricultural Biotechnology. 2023, 52 1-10. urn:issn:1878-8181 https://hdl.handle.net/11250/3087863 https://doi.org/10.1016/j.bcab.2023.102820 cristin:2171664 1-10 52 Biocatalysis and Agricultural Biotechnology Peer reviewed Journal article 2023 ftnofima https://doi.org/10.1016/j.bcab.2023.102820 2023-09-13T22:50:19Z Calanus finmarchicus is a crustacean currently used as a source of marine lipid. The lipids are extracted by enzymatic protein hydrolysis, while the remaining peptide fraction is regarded as a byproduct. In the present work, ten different commercial proteases and endogenous C. finmarchicus proteases were used to produce a set of 63 protein hydrolysates. Protease concentration and hydrolysis time were varied. Hydrolysates were characterized using size-exclusion chromatography and 1H nuclear magnetic resonance spectroscopy. Addition of commercial proteases had unremarkable effect on the yield and molecular weight distribution. This was attributed to the strong impact of endogenous enzymes dominating the hydrolysis process. However, multivariate classification based on 1H NMR spectra revealed subtle variations in composition of hydrolysates produced using different enzymes. The hydrolysates were further evaluated for DPP-IV inhibition and antioxidant activity. The hydrolysates showed significantly higher bioactivity than the unhydrolyzed control. A representative hydrolysate (CaFi55) was fractionated using semipreparative size-exclusion chromatography. A fraction consisting of short peptides with an average chain length of five amino acids (F2), was identified as a major contributor to the DPP-IV inhibitory activity (IC50 = 0.70 ± 0.07 mg/mL). Calanus finmarchicus as a novel source of health-promoting bioactive peptides: Enzymatic protein hydrolysis, characterization, and in vitro bioactivity publishedVersion Article in Journal/Newspaper Calanus finmarchicus Nofima Knowledge Archive (Brage) Biocatalysis and Agricultural Biotechnology 52 102820 |
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Open Polar |
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Nofima Knowledge Archive (Brage) |
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ftnofima |
language |
English |
description |
Calanus finmarchicus is a crustacean currently used as a source of marine lipid. The lipids are extracted by enzymatic protein hydrolysis, while the remaining peptide fraction is regarded as a byproduct. In the present work, ten different commercial proteases and endogenous C. finmarchicus proteases were used to produce a set of 63 protein hydrolysates. Protease concentration and hydrolysis time were varied. Hydrolysates were characterized using size-exclusion chromatography and 1H nuclear magnetic resonance spectroscopy. Addition of commercial proteases had unremarkable effect on the yield and molecular weight distribution. This was attributed to the strong impact of endogenous enzymes dominating the hydrolysis process. However, multivariate classification based on 1H NMR spectra revealed subtle variations in composition of hydrolysates produced using different enzymes. The hydrolysates were further evaluated for DPP-IV inhibition and antioxidant activity. The hydrolysates showed significantly higher bioactivity than the unhydrolyzed control. A representative hydrolysate (CaFi55) was fractionated using semipreparative size-exclusion chromatography. A fraction consisting of short peptides with an average chain length of five amino acids (F2), was identified as a major contributor to the DPP-IV inhibitory activity (IC50 = 0.70 ± 0.07 mg/mL). Calanus finmarchicus as a novel source of health-promoting bioactive peptides: Enzymatic protein hydrolysis, characterization, and in vitro bioactivity publishedVersion |
format |
Article in Journal/Newspaper |
author |
Matic, Josipa Bøgwald, Isak Tengstrand, Erik Rønning, Sissel Beate Afseth, Nils Kristian Wubshet, Sileshi Gizachew |
spellingShingle |
Matic, Josipa Bøgwald, Isak Tengstrand, Erik Rønning, Sissel Beate Afseth, Nils Kristian Wubshet, Sileshi Gizachew Calanus finmarchicus as a novel source of health-promoting bioactive peptides: Enzymatic protein hydrolysis, characterization, and in vitro bioactivity |
author_facet |
Matic, Josipa Bøgwald, Isak Tengstrand, Erik Rønning, Sissel Beate Afseth, Nils Kristian Wubshet, Sileshi Gizachew |
author_sort |
Matic, Josipa |
title |
Calanus finmarchicus as a novel source of health-promoting bioactive peptides: Enzymatic protein hydrolysis, characterization, and in vitro bioactivity |
title_short |
Calanus finmarchicus as a novel source of health-promoting bioactive peptides: Enzymatic protein hydrolysis, characterization, and in vitro bioactivity |
title_full |
Calanus finmarchicus as a novel source of health-promoting bioactive peptides: Enzymatic protein hydrolysis, characterization, and in vitro bioactivity |
title_fullStr |
Calanus finmarchicus as a novel source of health-promoting bioactive peptides: Enzymatic protein hydrolysis, characterization, and in vitro bioactivity |
title_full_unstemmed |
Calanus finmarchicus as a novel source of health-promoting bioactive peptides: Enzymatic protein hydrolysis, characterization, and in vitro bioactivity |
title_sort |
calanus finmarchicus as a novel source of health-promoting bioactive peptides: enzymatic protein hydrolysis, characterization, and in vitro bioactivity |
publishDate |
2023 |
url |
https://hdl.handle.net/11250/3087863 https://doi.org/10.1016/j.bcab.2023.102820 |
genre |
Calanus finmarchicus |
genre_facet |
Calanus finmarchicus |
op_source |
1-10 52 Biocatalysis and Agricultural Biotechnology |
op_relation |
Nofima AS: 202101 Nofima AS: 202102 Norges forskningsråd: 314599 Norges forskningsråd: 314111 Norges forskningsråd: 320086 Nofima AS: 13411 Biocatalysis and Agricultural Biotechnology. 2023, 52 1-10. urn:issn:1878-8181 https://hdl.handle.net/11250/3087863 https://doi.org/10.1016/j.bcab.2023.102820 cristin:2171664 |
op_doi |
https://doi.org/10.1016/j.bcab.2023.102820 |
container_title |
Biocatalysis and Agricultural Biotechnology |
container_volume |
52 |
container_start_page |
102820 |
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1779313516047499264 |