Oxidant-induced modifications in the mucosal transcriptome and circulating metabolome of Atlantic salmon
Here we report the molecular networks associated with the mucosal and systemic responses to peracetic acid (PAA), a candidate oxidative chemotherapeutic in Atlantic salmon (Salmo salar). Smolts were exposed to different therapeutic doses (0, 0.6 and 2.4 mg/L) of PAA for 5 min, followed by a re-expos...
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ftnofima:oai:nofima.brage.unit.no:11250/2681773 2023-05-15T15:31:46+02:00 Oxidant-induced modifications in the mucosal transcriptome and circulating metabolome of Atlantic salmon Lazado, Carlo C. Pedersen, Lars-Flemming Kjersti, Katrine Hånes Soleng, Malene Breiland, Mette Serine Wesmajervi Timmerhaus, Gerrit 2020 application/pdf https://hdl.handle.net/11250/2681773 https://doi.org/10.1016/j.aquatox.2020.105625 eng eng Fiskeri- og havbruksnæringens forskningsfinansiering: 901472 urn:issn:0166-445X https://hdl.handle.net/11250/2681773 https://doi.org/10.1016/j.aquatox.2020.105625 cristin:1827066 227 Aquatic Toxicology Peer reviewed Journal article 2020 ftnofima https://doi.org/10.1016/j.aquatox.2020.105625 2022-11-18T06:51:10Z Here we report the molecular networks associated with the mucosal and systemic responses to peracetic acid (PAA), a candidate oxidative chemotherapeutic in Atlantic salmon (Salmo salar). Smolts were exposed to different therapeutic doses (0, 0.6 and 2.4 mg/L) of PAA for 5 min, followed by a re-exposure to the same concentrations for 30 min 2 weeks later. PAA-exposed groups have higher external welfare score alterations, especially 2 weeks after the re-exposure. Cases of fin damage and scale loss were prevalent in the PAA-exposed groups. Transcriptomic profiling of mucosal tissues revealed that the skin had 12.5% more differentially regulated genes (DEGs) than the gills following PAA exposure. The largest cluster of DEGs, both in the skin and gills, were involved in tissue extracellular matrix and metabolism. There were 22 DEGs common to both mucosal tissues, which were represented primarily by genes involved in the biophysical integrity of the mucosal barrier, including cadherin, collagen I α 2 chain, mucin-2 and spondin 1a. The absence of significant clustering in the plasma metabolomes amongst the three treatment groups indicates that PAA treatment did not induce any global metabolomic disturbances. Nonetheless, five metabolites with known functions during oxidative stress were remarkably affected by PAA treatments such as citrulline, histidine, tryptophan, methionine and trans-4-hydroxyproline. Collectively, these results indicate that salmon were able to mount mucosal and systemic adaptive responses to therapeutic doses of PAA and that the molecules identified are potential markers for assessing the health and welfare consequences of oxidant exposure. publishedVersion Article in Journal/Newspaper Atlantic salmon Salmo salar Nofima Knowledge Archive (Brage) Paa ENVELOPE(-53.483,-53.483,66.017,66.017) Aquatic Toxicology 227 105625 |
institution |
Open Polar |
collection |
Nofima Knowledge Archive (Brage) |
op_collection_id |
ftnofima |
language |
English |
description |
Here we report the molecular networks associated with the mucosal and systemic responses to peracetic acid (PAA), a candidate oxidative chemotherapeutic in Atlantic salmon (Salmo salar). Smolts were exposed to different therapeutic doses (0, 0.6 and 2.4 mg/L) of PAA for 5 min, followed by a re-exposure to the same concentrations for 30 min 2 weeks later. PAA-exposed groups have higher external welfare score alterations, especially 2 weeks after the re-exposure. Cases of fin damage and scale loss were prevalent in the PAA-exposed groups. Transcriptomic profiling of mucosal tissues revealed that the skin had 12.5% more differentially regulated genes (DEGs) than the gills following PAA exposure. The largest cluster of DEGs, both in the skin and gills, were involved in tissue extracellular matrix and metabolism. There were 22 DEGs common to both mucosal tissues, which were represented primarily by genes involved in the biophysical integrity of the mucosal barrier, including cadherin, collagen I α 2 chain, mucin-2 and spondin 1a. The absence of significant clustering in the plasma metabolomes amongst the three treatment groups indicates that PAA treatment did not induce any global metabolomic disturbances. Nonetheless, five metabolites with known functions during oxidative stress were remarkably affected by PAA treatments such as citrulline, histidine, tryptophan, methionine and trans-4-hydroxyproline. Collectively, these results indicate that salmon were able to mount mucosal and systemic adaptive responses to therapeutic doses of PAA and that the molecules identified are potential markers for assessing the health and welfare consequences of oxidant exposure. publishedVersion |
format |
Article in Journal/Newspaper |
author |
Lazado, Carlo C. Pedersen, Lars-Flemming Kjersti, Katrine Hånes Soleng, Malene Breiland, Mette Serine Wesmajervi Timmerhaus, Gerrit |
spellingShingle |
Lazado, Carlo C. Pedersen, Lars-Flemming Kjersti, Katrine Hånes Soleng, Malene Breiland, Mette Serine Wesmajervi Timmerhaus, Gerrit Oxidant-induced modifications in the mucosal transcriptome and circulating metabolome of Atlantic salmon |
author_facet |
Lazado, Carlo C. Pedersen, Lars-Flemming Kjersti, Katrine Hånes Soleng, Malene Breiland, Mette Serine Wesmajervi Timmerhaus, Gerrit |
author_sort |
Lazado, Carlo C. |
title |
Oxidant-induced modifications in the mucosal transcriptome and circulating metabolome of Atlantic salmon |
title_short |
Oxidant-induced modifications in the mucosal transcriptome and circulating metabolome of Atlantic salmon |
title_full |
Oxidant-induced modifications in the mucosal transcriptome and circulating metabolome of Atlantic salmon |
title_fullStr |
Oxidant-induced modifications in the mucosal transcriptome and circulating metabolome of Atlantic salmon |
title_full_unstemmed |
Oxidant-induced modifications in the mucosal transcriptome and circulating metabolome of Atlantic salmon |
title_sort |
oxidant-induced modifications in the mucosal transcriptome and circulating metabolome of atlantic salmon |
publishDate |
2020 |
url |
https://hdl.handle.net/11250/2681773 https://doi.org/10.1016/j.aquatox.2020.105625 |
long_lat |
ENVELOPE(-53.483,-53.483,66.017,66.017) |
geographic |
Paa |
geographic_facet |
Paa |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
227 Aquatic Toxicology |
op_relation |
Fiskeri- og havbruksnæringens forskningsfinansiering: 901472 urn:issn:0166-445X https://hdl.handle.net/11250/2681773 https://doi.org/10.1016/j.aquatox.2020.105625 cristin:1827066 |
op_doi |
https://doi.org/10.1016/j.aquatox.2020.105625 |
container_title |
Aquatic Toxicology |
container_volume |
227 |
container_start_page |
105625 |
_version_ |
1766362287384297472 |