Experimental replacement of pig trachea with novel bioprosthesis from harp Seal

• Main Authors: Agathos, E.A., Tomos, P., Lachanas, E., Gakiopoulou, H., Pantopoulou, A., Perrea, D.
• Format: Article in Journal/Newspaper
• Language: unknown; English
• Published: 2010
• Subjects: Harp Seal
• Online Access: https://pergamos.lib.uoa.gr/uoa/dl/object/uoadl:3130065

Tracheal replacement has been a challenging problem for thoracic surgeons for over half of a century. We evaluated the in-vivo performance of a new tracheal bioprosthesis derived from Harp seal (Phoca groelandica) trachea that was fixed and preserved in 0.625% buffered glutaraldehyde solution for 3 months. Ten young male pigs weighing 27-32 kg (mean, 28.7 kg) underwent replacement of a tracheal segment with this new bioprosthesis. The length of replaced trachea was 1.8-2.4 cm (mean, 2.17 cm), representing 2-3 cartilage rings. All pigs survived the operation uneventfully. No immunosuppression drugs were used. The pigs eventually developed dyspnea and were euthanized on postoperative day 17-39 (mean, 30.8 days). Macroscopic and histological analysis showed an intact bioprosthesis but near-total occlusion of the native trachea by a ring of inflammatory infiltration at the site of distal anastomosis. More experiments involving a different concentration of the preservation agent, different management, and perhaps the use of bioengineering techniques are needed to improve the performance of this novel bioprosthesis. © 2010 SAGE Publications.