Enzymatic removal of carboxyl protecting groups. 1. Cleavage of the tert-butyl moiety
(Chemical Equation Presented) A recent discovery that a certain amino acid motif (GGG-(A)X-motif) in lipases and esterases determines their activity toward tertiary alcohols prompted us to investigate the use of these biocatalysts in the mild and selective removal of tert-butyl protecting groups in...
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ftnkunivathens:oai:lib.uoa.gr:uoadl:3067967 2024-02-11T09:58:47+01:00 Enzymatic removal of carboxyl protecting groups. 1. Cleavage of the tert-butyl moiety Schmidt, M. Barbayianni, E. Fotakopoulou, I. Höhne, M. Constantinou-Kokotou, V. Bornscheuer, U.T. Kokotos, G. 2005-01-01 https://pergamos.lib.uoa.gr/uoa/dl/object/uoadl:3067967 Αγγλικά English eng uoadl:3067967 https://pergamos.lib.uoa.gr/uoa/dl/object/uoadl:3067967 scientific_publication_article Επιστημονική δημοσίευση - Άρθρο Περιοδικού Scientific publication - Journal Article 2005 ftnkunivathens 2024-01-18T18:44:03Z (Chemical Equation Presented) A recent discovery that a certain amino acid motif (GGG-(A)X-motif) in lipases and esterases determines their activity toward tertiary alcohols prompted us to investigate the use of these biocatalysts in the mild and selective removal of tert-butyl protecting groups in amino acid derivatives and related compounds. An esterase from Bacillus subtilis (BsubpNBE) and lipase A from Candida antarctica (CAL-A) were identified as the most active enzymes, which hydrolyzed a range of tert-butyl esters of protected amino acids (e.g., Boc-Tyr-OtBu, Z-GABA-OtBu, Fmoc-GABA-O tBu) in good to high yields and left Boc, Z, and Fmoc-protecting groups intact. © 2005 American Chemical Society. Article in Journal/Newspaper Antarc* Antarctica Pergamos - Library and Information Center of National and Kapodistrian University of Athens |
institution |
Open Polar |
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Pergamos - Library and Information Center of National and Kapodistrian University of Athens |
op_collection_id |
ftnkunivathens |
language |
English |
description |
(Chemical Equation Presented) A recent discovery that a certain amino acid motif (GGG-(A)X-motif) in lipases and esterases determines their activity toward tertiary alcohols prompted us to investigate the use of these biocatalysts in the mild and selective removal of tert-butyl protecting groups in amino acid derivatives and related compounds. An esterase from Bacillus subtilis (BsubpNBE) and lipase A from Candida antarctica (CAL-A) were identified as the most active enzymes, which hydrolyzed a range of tert-butyl esters of protected amino acids (e.g., Boc-Tyr-OtBu, Z-GABA-OtBu, Fmoc-GABA-O tBu) in good to high yields and left Boc, Z, and Fmoc-protecting groups intact. © 2005 American Chemical Society. |
format |
Article in Journal/Newspaper |
author |
Schmidt, M. Barbayianni, E. Fotakopoulou, I. Höhne, M. Constantinou-Kokotou, V. Bornscheuer, U.T. Kokotos, G. |
spellingShingle |
Schmidt, M. Barbayianni, E. Fotakopoulou, I. Höhne, M. Constantinou-Kokotou, V. Bornscheuer, U.T. Kokotos, G. Enzymatic removal of carboxyl protecting groups. 1. Cleavage of the tert-butyl moiety |
author_facet |
Schmidt, M. Barbayianni, E. Fotakopoulou, I. Höhne, M. Constantinou-Kokotou, V. Bornscheuer, U.T. Kokotos, G. |
author_sort |
Schmidt, M. |
title |
Enzymatic removal of carboxyl protecting groups. 1. Cleavage of the tert-butyl moiety |
title_short |
Enzymatic removal of carboxyl protecting groups. 1. Cleavage of the tert-butyl moiety |
title_full |
Enzymatic removal of carboxyl protecting groups. 1. Cleavage of the tert-butyl moiety |
title_fullStr |
Enzymatic removal of carboxyl protecting groups. 1. Cleavage of the tert-butyl moiety |
title_full_unstemmed |
Enzymatic removal of carboxyl protecting groups. 1. Cleavage of the tert-butyl moiety |
title_sort |
enzymatic removal of carboxyl protecting groups. 1. cleavage of the tert-butyl moiety |
publishDate |
2005 |
url |
https://pergamos.lib.uoa.gr/uoa/dl/object/uoadl:3067967 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
uoadl:3067967 https://pergamos.lib.uoa.gr/uoa/dl/object/uoadl:3067967 |
_version_ |
1790594545571856384 |