Application of the Biolog EcoPlate™ technique for assessing the microbial metabolic diversity in moss profiles of continental Antarctica
Little is known about metabolic capabilities of microbial assemblages in continental Antarctic to utilize diverse carbon sources within moss profiles consisting of live, senescent, and dead moss tissues in different stages of decomposition. In the present study, microbial metabolic diversity was det...
Published in: | Polar Science |
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Online Access: | https://nipr.repo.nii.ac.jp/?action=repository_uri&item_id=17375 http://id.nii.ac.jp/1291/00017232/ |
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ftnipr:oai:nipr.repo.nii.ac.jp:00017375 2023-06-11T04:05:44+02:00 Application of the Biolog EcoPlate™ technique for assessing the microbial metabolic diversity in moss profiles of continental Antarctica 2023-03 https://nipr.repo.nii.ac.jp/?action=repository_uri&item_id=17375 http://id.nii.ac.jp/1291/00017232/ en eng https://doi.org/10.1016/j.polar.2023.100924 https://nipr.repo.nii.ac.jp/?action=repository_uri&item_id=17375 http://id.nii.ac.jp/1291/00017232/ Polar Science, 35, 100924(2023-03) 18739652 Biolog EcoPlate™ Bryophyte Bryum pseudotriquetrum Community level physiological profiling Substrate utilization Journal Article 2023 ftnipr https://doi.org/10.1016/j.polar.2023.100924 2023-04-29T19:34:29Z Little is known about metabolic capabilities of microbial assemblages in continental Antarctic to utilize diverse carbon sources within moss profiles consisting of live, senescent, and dead moss tissues in different stages of decomposition. In the present study, microbial metabolic diversity was determined using Biolog EcoPlate™ for samples of Bryum pseudotriquetrum/B. archangelicum complex collected at five sites located in coastal outcrops of the Lützow-Holm Bay area (Queen Maud Land), East continental Antarctica, and effects of chemical, biological, and physical properties of the moss tissues were explored. The average well-color development, the number of substrates utilized, Simpson's diversity index and equitability of the microbial substrate utilization, and the utilization of carbohydrates, amino acids/amines, carboxylic acids, and polymers varied significantly among the five sites and among four layers of moss profiles. Principal component analysis showed that the utilization of L-asparagine, L-arginine, D-galacturonic acid, Tween 40, Tween 80, and D-mannitol mainly contributed to the variance in the microbial metabolic diversity between sites and moss profiles. The linear mixed models indicated that the relative content of recalcitrant organic components, the number of fungal species, and the water content of moss tissues affected the diversity of microbial utilization of carbon sources. Article in Journal/Newspaper Antarc* Antarctic Antarctica Polar Science Polar Science Queen Maud Land National Institute of Polar Research Repository, Japan Antarctic Lützow-Holm Bay ENVELOPE(38.000,38.000,-69.500,-69.500) Queen Maud Land ENVELOPE(12.000,12.000,-72.500,-72.500) Polar Science 35 100924 |
institution |
Open Polar |
collection |
National Institute of Polar Research Repository, Japan |
op_collection_id |
ftnipr |
language |
English |
topic |
Biolog EcoPlate™ Bryophyte Bryum pseudotriquetrum Community level physiological profiling Substrate utilization |
spellingShingle |
Biolog EcoPlate™ Bryophyte Bryum pseudotriquetrum Community level physiological profiling Substrate utilization Application of the Biolog EcoPlate™ technique for assessing the microbial metabolic diversity in moss profiles of continental Antarctica |
topic_facet |
Biolog EcoPlate™ Bryophyte Bryum pseudotriquetrum Community level physiological profiling Substrate utilization |
description |
Little is known about metabolic capabilities of microbial assemblages in continental Antarctic to utilize diverse carbon sources within moss profiles consisting of live, senescent, and dead moss tissues in different stages of decomposition. In the present study, microbial metabolic diversity was determined using Biolog EcoPlate™ for samples of Bryum pseudotriquetrum/B. archangelicum complex collected at five sites located in coastal outcrops of the Lützow-Holm Bay area (Queen Maud Land), East continental Antarctica, and effects of chemical, biological, and physical properties of the moss tissues were explored. The average well-color development, the number of substrates utilized, Simpson's diversity index and equitability of the microbial substrate utilization, and the utilization of carbohydrates, amino acids/amines, carboxylic acids, and polymers varied significantly among the five sites and among four layers of moss profiles. Principal component analysis showed that the utilization of L-asparagine, L-arginine, D-galacturonic acid, Tween 40, Tween 80, and D-mannitol mainly contributed to the variance in the microbial metabolic diversity between sites and moss profiles. The linear mixed models indicated that the relative content of recalcitrant organic components, the number of fungal species, and the water content of moss tissues affected the diversity of microbial utilization of carbon sources. |
format |
Article in Journal/Newspaper |
title |
Application of the Biolog EcoPlate™ technique for assessing the microbial metabolic diversity in moss profiles of continental Antarctica |
title_short |
Application of the Biolog EcoPlate™ technique for assessing the microbial metabolic diversity in moss profiles of continental Antarctica |
title_full |
Application of the Biolog EcoPlate™ technique for assessing the microbial metabolic diversity in moss profiles of continental Antarctica |
title_fullStr |
Application of the Biolog EcoPlate™ technique for assessing the microbial metabolic diversity in moss profiles of continental Antarctica |
title_full_unstemmed |
Application of the Biolog EcoPlate™ technique for assessing the microbial metabolic diversity in moss profiles of continental Antarctica |
title_sort |
application of the biolog ecoplate™ technique for assessing the microbial metabolic diversity in moss profiles of continental antarctica |
publishDate |
2023 |
url |
https://nipr.repo.nii.ac.jp/?action=repository_uri&item_id=17375 http://id.nii.ac.jp/1291/00017232/ |
long_lat |
ENVELOPE(38.000,38.000,-69.500,-69.500) ENVELOPE(12.000,12.000,-72.500,-72.500) |
geographic |
Antarctic Lützow-Holm Bay Queen Maud Land |
geographic_facet |
Antarctic Lützow-Holm Bay Queen Maud Land |
genre |
Antarc* Antarctic Antarctica Polar Science Polar Science Queen Maud Land |
genre_facet |
Antarc* Antarctic Antarctica Polar Science Polar Science Queen Maud Land |
op_relation |
https://doi.org/10.1016/j.polar.2023.100924 https://nipr.repo.nii.ac.jp/?action=repository_uri&item_id=17375 http://id.nii.ac.jp/1291/00017232/ Polar Science, 35, 100924(2023-03) 18739652 |
op_doi |
https://doi.org/10.1016/j.polar.2023.100924 |
container_title |
Polar Science |
container_volume |
35 |
container_start_page |
100924 |
_version_ |
1768377353786884096 |