Antibiotic resistance and cold-adaptive enzymes of antarctic culturable bacteria from King George Island
Cryotolerance in microorganisms from polar regions provides their survival through alternating freezing and thawing cycles. Our research was aimed at biodiversity analysis of culturable bacteria from the King George Island near Antarctica including their antibiotic resistance and cold-adaptive enzym...
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ftnipr:oai:nipr.repo.nii.ac.jp:00016903 2023-05-15T13:49:00+02:00 Antibiotic resistance and cold-adaptive enzymes of antarctic culturable bacteria from King George Island 2022-03 https://nipr.repo.nii.ac.jp/?action=repository_uri&item_id=16903 http://id.nii.ac.jp/1291/00016772/ en eng https://doi.org/10.1016/j.polar.2021.100756 https://nipr.repo.nii.ac.jp/?action=repository_uri&item_id=16903 http://id.nii.ac.jp/1291/00016772/ Polar Science, 31, 100756(2022-03) 18739652 Culturable psychrophilic eubacteria 16S rRNA gene phylogenetic analysis Plasmids Alkaline phosphatases Lipases Exo- and restriction endonucleases Journal Article 2022 ftnipr https://doi.org/10.1016/j.polar.2021.100756 2022-12-03T19:43:26Z Cryotolerance in microorganisms from polar regions provides their survival through alternating freezing and thawing cycles. Our research was aimed at biodiversity analysis of culturable bacteria from the King George Island near Antarctica including their antibiotic resistance and cold-adaptive enzymes. Average bacterial density was estimated as 3 × 106 cells/mL but the number of colony-forming units (CFU) was essentially lower - (3.2–9.2)x103 cells/mL. Totally, 83 stable strains were isolated and identified using phylogenetic analysis of 16S rRNA gene nucleotide sequences and phenotypic traits. Main groups were the Gram-negative Pseudomonas and Duganella species as well as Gram-positive Arthrobacter spp. Besides, eubacteria Psychrobacter, Flavobacterium, Clavibacter, Aquaspirillum, Polaromonas, Actinibacterium, Shewanella, Kocuria and families Comamonadacea, Actinobacteridae were found. Genetic diversity of the bacteria was elevated with plasmids of different lengths in 33 strains but no correlation between the plasmids content and antibiotic resistance was found. Among cold-adapted enzymes antioxidant catalases (91.5%), peroxidases (57.5%), alkaline phosphatases (42.2%) prevailed; unspecific exonucleases were revealed in 16.9% strains and restriction endonucleases of type II - in 8.4% strains only. Lypases were detected in 24 strains (28.9%) and could facilitate membrane fluidity at low temperatures. Despite the antibiotic resistance the Antarctic cultivable eubacteria are mainly not pathogenic and may serve as sources of plasmids and cold-adapted enzymes for biotechnology. Article in Journal/Newspaper Antarc* Antarctic Antarctica King George Island Polar Science Polar Science National Institute of Polar Research Repository, Japan Antarctic The Antarctic King George Island Polar Science 31 100756 |
institution |
Open Polar |
collection |
National Institute of Polar Research Repository, Japan |
op_collection_id |
ftnipr |
language |
English |
topic |
Culturable psychrophilic eubacteria 16S rRNA gene phylogenetic analysis Plasmids Alkaline phosphatases Lipases Exo- and restriction endonucleases |
spellingShingle |
Culturable psychrophilic eubacteria 16S rRNA gene phylogenetic analysis Plasmids Alkaline phosphatases Lipases Exo- and restriction endonucleases Antibiotic resistance and cold-adaptive enzymes of antarctic culturable bacteria from King George Island |
topic_facet |
Culturable psychrophilic eubacteria 16S rRNA gene phylogenetic analysis Plasmids Alkaline phosphatases Lipases Exo- and restriction endonucleases |
description |
Cryotolerance in microorganisms from polar regions provides their survival through alternating freezing and thawing cycles. Our research was aimed at biodiversity analysis of culturable bacteria from the King George Island near Antarctica including their antibiotic resistance and cold-adaptive enzymes. Average bacterial density was estimated as 3 × 106 cells/mL but the number of colony-forming units (CFU) was essentially lower - (3.2–9.2)x103 cells/mL. Totally, 83 stable strains were isolated and identified using phylogenetic analysis of 16S rRNA gene nucleotide sequences and phenotypic traits. Main groups were the Gram-negative Pseudomonas and Duganella species as well as Gram-positive Arthrobacter spp. Besides, eubacteria Psychrobacter, Flavobacterium, Clavibacter, Aquaspirillum, Polaromonas, Actinibacterium, Shewanella, Kocuria and families Comamonadacea, Actinobacteridae were found. Genetic diversity of the bacteria was elevated with plasmids of different lengths in 33 strains but no correlation between the plasmids content and antibiotic resistance was found. Among cold-adapted enzymes antioxidant catalases (91.5%), peroxidases (57.5%), alkaline phosphatases (42.2%) prevailed; unspecific exonucleases were revealed in 16.9% strains and restriction endonucleases of type II - in 8.4% strains only. Lypases were detected in 24 strains (28.9%) and could facilitate membrane fluidity at low temperatures. Despite the antibiotic resistance the Antarctic cultivable eubacteria are mainly not pathogenic and may serve as sources of plasmids and cold-adapted enzymes for biotechnology. |
format |
Article in Journal/Newspaper |
title |
Antibiotic resistance and cold-adaptive enzymes of antarctic culturable bacteria from King George Island |
title_short |
Antibiotic resistance and cold-adaptive enzymes of antarctic culturable bacteria from King George Island |
title_full |
Antibiotic resistance and cold-adaptive enzymes of antarctic culturable bacteria from King George Island |
title_fullStr |
Antibiotic resistance and cold-adaptive enzymes of antarctic culturable bacteria from King George Island |
title_full_unstemmed |
Antibiotic resistance and cold-adaptive enzymes of antarctic culturable bacteria from King George Island |
title_sort |
antibiotic resistance and cold-adaptive enzymes of antarctic culturable bacteria from king george island |
publishDate |
2022 |
url |
https://nipr.repo.nii.ac.jp/?action=repository_uri&item_id=16903 http://id.nii.ac.jp/1291/00016772/ |
geographic |
Antarctic The Antarctic King George Island |
geographic_facet |
Antarctic The Antarctic King George Island |
genre |
Antarc* Antarctic Antarctica King George Island Polar Science Polar Science |
genre_facet |
Antarc* Antarctic Antarctica King George Island Polar Science Polar Science |
op_relation |
https://doi.org/10.1016/j.polar.2021.100756 https://nipr.repo.nii.ac.jp/?action=repository_uri&item_id=16903 http://id.nii.ac.jp/1291/00016772/ Polar Science, 31, 100756(2022-03) 18739652 |
op_doi |
https://doi.org/10.1016/j.polar.2021.100756 |
container_title |
Polar Science |
container_volume |
31 |
container_start_page |
100756 |
_version_ |
1766250399302418432 |