Partial characterization of an antifreeze protein (CRY-c) from Cryobacterium psychrotolerans MLB-29 of Arctic glacier cryoconite
Antifreeze proteins (AFPs) play important roles in the survival of microorganisms existing in the cold environment. One of the several AFPs from an Arctic glacier bacterial strain which in our previous study showed thermal hysteresis (TH) and ice recrystallization inhibition (IRI) activities has bee...
Published in: | Polar Science |
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2021
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ftnipr:oai:nipr.repo.nii.ac.jp:00016496 2023-05-15T14:44:35+02:00 Partial characterization of an antifreeze protein (CRY-c) from Cryobacterium psychrotolerans MLB-29 of Arctic glacier cryoconite 2021-06 https://nipr.repo.nii.ac.jp/?action=repository_uri&item_id=16496 http://id.nii.ac.jp/1291/00016367/ en eng https://doi.org/10.1016/j.polar.2021.100661 https://nipr.repo.nii.ac.jp/?action=repository_uri&item_id=16496 http://id.nii.ac.jp/1291/00016367/ Polar Science, 28, 100661(2021-06) 18739652 Antifreeze protein Arctic bacteria Cryoconite Glacier nanoLC-ESI-MS/MS Journal Article 2021 ftnipr https://doi.org/10.1016/j.polar.2021.100661 2022-12-03T19:43:21Z Antifreeze proteins (AFPs) play important roles in the survival of microorganisms existing in the cold environment. One of the several AFPs from an Arctic glacier bacterial strain which in our previous study showed thermal hysteresis (TH) and ice recrystallization inhibition (IRI) activities has been characterized partially in the present study. The cry-c AFP from the Svalbard Arctic cryoconite strain Cry-c MLB-29 belonging to the genus Cryobacterium has been characterized by ESI-Q-TOF-MS that revealed its molecular mass as 22.14 kDa. Multi-enzymatic limited digestion and nano-LC-ESI-MS/MS analyses revealed 228 amino acid residues in 21 peptide fragments. First 20 amino acid residues of the N-terminus of cry-c AFP has been deduced. Glycine and alalnine-rich sequences and the NXS motifs in several peptide fragments have been found out. Selected peptide fragments of the AFP have been modeled by PEP-FOLD and the structure-function correlation discussed in view of its antifreeze property. Article in Journal/Newspaper Arctic glacier Polar Science Polar Science Svalbard National Institute of Polar Research Repository, Japan Arctic Svalbard Polar Science 28 100661 |
institution |
Open Polar |
collection |
National Institute of Polar Research Repository, Japan |
op_collection_id |
ftnipr |
language |
English |
topic |
Antifreeze protein Arctic bacteria Cryoconite Glacier nanoLC-ESI-MS/MS |
spellingShingle |
Antifreeze protein Arctic bacteria Cryoconite Glacier nanoLC-ESI-MS/MS Partial characterization of an antifreeze protein (CRY-c) from Cryobacterium psychrotolerans MLB-29 of Arctic glacier cryoconite |
topic_facet |
Antifreeze protein Arctic bacteria Cryoconite Glacier nanoLC-ESI-MS/MS |
description |
Antifreeze proteins (AFPs) play important roles in the survival of microorganisms existing in the cold environment. One of the several AFPs from an Arctic glacier bacterial strain which in our previous study showed thermal hysteresis (TH) and ice recrystallization inhibition (IRI) activities has been characterized partially in the present study. The cry-c AFP from the Svalbard Arctic cryoconite strain Cry-c MLB-29 belonging to the genus Cryobacterium has been characterized by ESI-Q-TOF-MS that revealed its molecular mass as 22.14 kDa. Multi-enzymatic limited digestion and nano-LC-ESI-MS/MS analyses revealed 228 amino acid residues in 21 peptide fragments. First 20 amino acid residues of the N-terminus of cry-c AFP has been deduced. Glycine and alalnine-rich sequences and the NXS motifs in several peptide fragments have been found out. Selected peptide fragments of the AFP have been modeled by PEP-FOLD and the structure-function correlation discussed in view of its antifreeze property. |
format |
Article in Journal/Newspaper |
title |
Partial characterization of an antifreeze protein (CRY-c) from Cryobacterium psychrotolerans MLB-29 of Arctic glacier cryoconite |
title_short |
Partial characterization of an antifreeze protein (CRY-c) from Cryobacterium psychrotolerans MLB-29 of Arctic glacier cryoconite |
title_full |
Partial characterization of an antifreeze protein (CRY-c) from Cryobacterium psychrotolerans MLB-29 of Arctic glacier cryoconite |
title_fullStr |
Partial characterization of an antifreeze protein (CRY-c) from Cryobacterium psychrotolerans MLB-29 of Arctic glacier cryoconite |
title_full_unstemmed |
Partial characterization of an antifreeze protein (CRY-c) from Cryobacterium psychrotolerans MLB-29 of Arctic glacier cryoconite |
title_sort |
partial characterization of an antifreeze protein (cry-c) from cryobacterium psychrotolerans mlb-29 of arctic glacier cryoconite |
publishDate |
2021 |
url |
https://nipr.repo.nii.ac.jp/?action=repository_uri&item_id=16496 http://id.nii.ac.jp/1291/00016367/ |
geographic |
Arctic Svalbard |
geographic_facet |
Arctic Svalbard |
genre |
Arctic glacier Polar Science Polar Science Svalbard |
genre_facet |
Arctic glacier Polar Science Polar Science Svalbard |
op_relation |
https://doi.org/10.1016/j.polar.2021.100661 https://nipr.repo.nii.ac.jp/?action=repository_uri&item_id=16496 http://id.nii.ac.jp/1291/00016367/ Polar Science, 28, 100661(2021-06) 18739652 |
op_doi |
https://doi.org/10.1016/j.polar.2021.100661 |
container_title |
Polar Science |
container_volume |
28 |
container_start_page |
100661 |
_version_ |
1766316079524610048 |