Identification of Dalapon degrading bacterial strain, Psychrobacter sp. TaeBurcu001 isolated from Antarctica
Extreme microorganism has gained interest because of their special contribution to the environmental and industrial biotechnology, particularly psychrophilic microorganisms which can live and thrive at lower temperatures. They produce enzymes with great potential to adapt to extreme conditions. In t...
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ftnipr:oai:nipr.repo.nii.ac.jp:00016493 2023-05-15T13:49:00+02:00 Identification of Dalapon degrading bacterial strain, Psychrobacter sp. TaeBurcu001 isolated from Antarctica 2021-06 https://nipr.repo.nii.ac.jp/?action=repository_uri&item_id=16493 http://id.nii.ac.jp/1291/00016364/ en eng https://doi.org/10.1016/j.polar.2021.100656 https://nipr.repo.nii.ac.jp/?action=repository_uri&item_id=16493 http://id.nii.ac.jp/1291/00016364/ Polar Science, 28, 100656(2021-06) 18739652 Antarctica Polar microorganisms Halogenated compounds Herbicide degrading bacteria 16S rRNA sequencing Journal Article 2021 ftnipr https://doi.org/10.1016/j.polar.2021.100656 2022-12-03T19:43:21Z Extreme microorganism has gained interest because of their special contribution to the environmental and industrial biotechnology, particularly psychrophilic microorganisms which can live and thrive at lower temperatures. They produce enzymes with great potential to adapt to extreme conditions. In this study, a novel psychrophilic bacterial strain designated as TaeBurcu001 grew (optimally at 5 °C) on a chemically defined medium containing dalapon (refer to as 2,2-dichloropropionic acid, 2,2-DCP) as the sole carbon source. TaeBurcu001 was isolated from Galindez Island, Antarctica. Phylogenetic analysis based on 16S rRNA gene sequence (GeneBank ID MN061637) showed that TaeBurcu001 was closely related to members of the genus Psychrobacter (99%). Comparisons of phenotypic and biochemical characteristics between the isolate and the other known Psychrobacter species showed that they are very similar. Chloride ion release was detected using colorimetric assay with maximum value recorded at 0.27 mmol/L in 30 mM of 2,2-DCP. Furthermore, the partial dehalogenase sequence of Psychrobacter TaeBurcu001 (GeneBank ID MW311072) showed a very high similarity (88%) to the previously reported 2-haloacid dehalogenase (DehE) from Rhizobium sp. (GeneBank ID CAA75671). To the best of our knowledge, this is the first study reporting on the isolation and characterisation of psychrophilic bacteria able to grow on halogenated compounds as a carbon source. Results are expected to gain new insight for further studies to uncover the full potential of these microorganisms to be applied in environmental processes and the remediation of contaminated ecosystems. Article in Journal/Newspaper Antarc* Antarctica Galindez Island Polar Science Polar Science National Institute of Polar Research Repository, Japan Galindez ENVELOPE(-64.248,-64.248,-65.250,-65.250) Galindez Island ENVELOPE(-64.267,-64.267,-65.250,-65.250) Polar Science 28 100656 |
institution |
Open Polar |
collection |
National Institute of Polar Research Repository, Japan |
op_collection_id |
ftnipr |
language |
English |
topic |
Antarctica Polar microorganisms Halogenated compounds Herbicide degrading bacteria 16S rRNA sequencing |
spellingShingle |
Antarctica Polar microorganisms Halogenated compounds Herbicide degrading bacteria 16S rRNA sequencing Identification of Dalapon degrading bacterial strain, Psychrobacter sp. TaeBurcu001 isolated from Antarctica |
topic_facet |
Antarctica Polar microorganisms Halogenated compounds Herbicide degrading bacteria 16S rRNA sequencing |
description |
Extreme microorganism has gained interest because of their special contribution to the environmental and industrial biotechnology, particularly psychrophilic microorganisms which can live and thrive at lower temperatures. They produce enzymes with great potential to adapt to extreme conditions. In this study, a novel psychrophilic bacterial strain designated as TaeBurcu001 grew (optimally at 5 °C) on a chemically defined medium containing dalapon (refer to as 2,2-dichloropropionic acid, 2,2-DCP) as the sole carbon source. TaeBurcu001 was isolated from Galindez Island, Antarctica. Phylogenetic analysis based on 16S rRNA gene sequence (GeneBank ID MN061637) showed that TaeBurcu001 was closely related to members of the genus Psychrobacter (99%). Comparisons of phenotypic and biochemical characteristics between the isolate and the other known Psychrobacter species showed that they are very similar. Chloride ion release was detected using colorimetric assay with maximum value recorded at 0.27 mmol/L in 30 mM of 2,2-DCP. Furthermore, the partial dehalogenase sequence of Psychrobacter TaeBurcu001 (GeneBank ID MW311072) showed a very high similarity (88%) to the previously reported 2-haloacid dehalogenase (DehE) from Rhizobium sp. (GeneBank ID CAA75671). To the best of our knowledge, this is the first study reporting on the isolation and characterisation of psychrophilic bacteria able to grow on halogenated compounds as a carbon source. Results are expected to gain new insight for further studies to uncover the full potential of these microorganisms to be applied in environmental processes and the remediation of contaminated ecosystems. |
format |
Article in Journal/Newspaper |
title |
Identification of Dalapon degrading bacterial strain, Psychrobacter sp. TaeBurcu001 isolated from Antarctica |
title_short |
Identification of Dalapon degrading bacterial strain, Psychrobacter sp. TaeBurcu001 isolated from Antarctica |
title_full |
Identification of Dalapon degrading bacterial strain, Psychrobacter sp. TaeBurcu001 isolated from Antarctica |
title_fullStr |
Identification of Dalapon degrading bacterial strain, Psychrobacter sp. TaeBurcu001 isolated from Antarctica |
title_full_unstemmed |
Identification of Dalapon degrading bacterial strain, Psychrobacter sp. TaeBurcu001 isolated from Antarctica |
title_sort |
identification of dalapon degrading bacterial strain, psychrobacter sp. taeburcu001 isolated from antarctica |
publishDate |
2021 |
url |
https://nipr.repo.nii.ac.jp/?action=repository_uri&item_id=16493 http://id.nii.ac.jp/1291/00016364/ |
long_lat |
ENVELOPE(-64.248,-64.248,-65.250,-65.250) ENVELOPE(-64.267,-64.267,-65.250,-65.250) |
geographic |
Galindez Galindez Island |
geographic_facet |
Galindez Galindez Island |
genre |
Antarc* Antarctica Galindez Island Polar Science Polar Science |
genre_facet |
Antarc* Antarctica Galindez Island Polar Science Polar Science |
op_relation |
https://doi.org/10.1016/j.polar.2021.100656 https://nipr.repo.nii.ac.jp/?action=repository_uri&item_id=16493 http://id.nii.ac.jp/1291/00016364/ Polar Science, 28, 100656(2021-06) 18739652 |
op_doi |
https://doi.org/10.1016/j.polar.2021.100656 |
container_title |
Polar Science |
container_volume |
28 |
container_start_page |
100656 |
_version_ |
1766250395711045632 |