Photosynthetic response and DNA mutation of tropical, temperate and polar Chlorella under short-term UVR stress
Changes in photosynthetic efficiency of Photosystem II can be used as an early stress indicator in phototrophs. In this study, chlorophyll fluorescence, measured by a Pulse-Amplitude Modulated Fluorometer (PAM), was used to determine the photosynthetic performance of tropical, temperate, Antarctic a...
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ftnipr:oai:nipr.repo.nii.ac.jp:00015616 2023-05-15T13:49:00+02:00 Photosynthetic response and DNA mutation of tropical, temperate and polar Chlorella under short-term UVR stress 2019-06 https://nipr.repo.nii.ac.jp/?action=repository_uri&item_id=15616 http://id.nii.ac.jp/1291/00015511/ en eng https://doi.org/10.1016/j.polar.2018.12.004 https://nipr.repo.nii.ac.jp/?action=repository_uri&item_id=15616 http://id.nii.ac.jp/1291/00015511/ Polar Science, 20P1, 35-44(2019-06) 18739652 Chlorella Photosynthesis PAM fluorometry Random amplified polymorphic DNA (RAPD) Ultraviolet radiation (UVR) Journal Article 2019 ftnipr https://doi.org/10.1016/j.polar.2018.12.004 2022-12-03T19:43:10Z Changes in photosynthetic efficiency of Photosystem II can be used as an early stress indicator in phototrophs. In this study, chlorophyll fluorescence, measured by a Pulse-Amplitude Modulated Fluorometer (PAM), was used to determine the photosynthetic performance of tropical, temperate, Antarctic and Arctic Chlorella in response to short-term acute ultraviolet radiation (UVR) stress, with measurements of maximum quantum yield (Fv/Fm), photosynthetic efficiency (α), maximum electron transport rate (rETRm) and photoadaptive index (Ek). Three light treatments were conducted over a continuous, five-hour duration: (i) control subjected only to photosynthetic active radiation (PAR), (ii) PAR + UVA (UVA) and (iii) PAR + UVA + UVB (UVR). Tropical Chlorella showed better adaptive ability to UVA stress compared to strains from temperate and polar regions. UVB stress caused significant photosynthetic dysfunction in all samples, with polar strains showing a lower inhibition (about 40%) compared to the tropical strain (about 98%). Photosynthetic responses in Chlorella towards UVR are possibly origin dependent. DNA mutation induced by both UVA and UVR treatments was revealed by Random Amplified Polymorphic DNA (RAPD) analysis. Out of sixty RAPD primers tested, two primers: S33 (polymorphism degree 44.83%) and S90 (polymorphism degree 38.71%) were chosen as potential primers to conduct genomic study of UV stress in microalgae. Article in Journal/Newspaper Antarc* Antarctic Arctic Polar Science Polar Science National Institute of Polar Research Repository, Japan Arctic Antarctic Polar Science 20 35 44 |
institution |
Open Polar |
collection |
National Institute of Polar Research Repository, Japan |
op_collection_id |
ftnipr |
language |
English |
topic |
Chlorella Photosynthesis PAM fluorometry Random amplified polymorphic DNA (RAPD) Ultraviolet radiation (UVR) |
spellingShingle |
Chlorella Photosynthesis PAM fluorometry Random amplified polymorphic DNA (RAPD) Ultraviolet radiation (UVR) Photosynthetic response and DNA mutation of tropical, temperate and polar Chlorella under short-term UVR stress |
topic_facet |
Chlorella Photosynthesis PAM fluorometry Random amplified polymorphic DNA (RAPD) Ultraviolet radiation (UVR) |
description |
Changes in photosynthetic efficiency of Photosystem II can be used as an early stress indicator in phototrophs. In this study, chlorophyll fluorescence, measured by a Pulse-Amplitude Modulated Fluorometer (PAM), was used to determine the photosynthetic performance of tropical, temperate, Antarctic and Arctic Chlorella in response to short-term acute ultraviolet radiation (UVR) stress, with measurements of maximum quantum yield (Fv/Fm), photosynthetic efficiency (α), maximum electron transport rate (rETRm) and photoadaptive index (Ek). Three light treatments were conducted over a continuous, five-hour duration: (i) control subjected only to photosynthetic active radiation (PAR), (ii) PAR + UVA (UVA) and (iii) PAR + UVA + UVB (UVR). Tropical Chlorella showed better adaptive ability to UVA stress compared to strains from temperate and polar regions. UVB stress caused significant photosynthetic dysfunction in all samples, with polar strains showing a lower inhibition (about 40%) compared to the tropical strain (about 98%). Photosynthetic responses in Chlorella towards UVR are possibly origin dependent. DNA mutation induced by both UVA and UVR treatments was revealed by Random Amplified Polymorphic DNA (RAPD) analysis. Out of sixty RAPD primers tested, two primers: S33 (polymorphism degree 44.83%) and S90 (polymorphism degree 38.71%) were chosen as potential primers to conduct genomic study of UV stress in microalgae. |
format |
Article in Journal/Newspaper |
title |
Photosynthetic response and DNA mutation of tropical, temperate and polar Chlorella under short-term UVR stress |
title_short |
Photosynthetic response and DNA mutation of tropical, temperate and polar Chlorella under short-term UVR stress |
title_full |
Photosynthetic response and DNA mutation of tropical, temperate and polar Chlorella under short-term UVR stress |
title_fullStr |
Photosynthetic response and DNA mutation of tropical, temperate and polar Chlorella under short-term UVR stress |
title_full_unstemmed |
Photosynthetic response and DNA mutation of tropical, temperate and polar Chlorella under short-term UVR stress |
title_sort |
photosynthetic response and dna mutation of tropical, temperate and polar chlorella under short-term uvr stress |
publishDate |
2019 |
url |
https://nipr.repo.nii.ac.jp/?action=repository_uri&item_id=15616 http://id.nii.ac.jp/1291/00015511/ |
geographic |
Arctic Antarctic |
geographic_facet |
Arctic Antarctic |
genre |
Antarc* Antarctic Arctic Polar Science Polar Science |
genre_facet |
Antarc* Antarctic Arctic Polar Science Polar Science |
op_relation |
https://doi.org/10.1016/j.polar.2018.12.004 https://nipr.repo.nii.ac.jp/?action=repository_uri&item_id=15616 http://id.nii.ac.jp/1291/00015511/ Polar Science, 20P1, 35-44(2019-06) 18739652 |
op_doi |
https://doi.org/10.1016/j.polar.2018.12.004 |
container_title |
Polar Science |
container_volume |
20 |
container_start_page |
35 |
op_container_end_page |
44 |
_version_ |
1766250385794662400 |