IDENTIFICATION AND CHARACTERIZATION OF A 9-CIS-HEXADECENOIC ACID CIS-TRANS ISOMERASE FROM A PSYCHROTROPHIC BACTERIUM, PSEUDOMONAS SP. STRAIN E-3 (18th Symposium on Polar Biology)
A cell-free extract of Pseudomonas sp. strain E-3 (Pseudomonas E-3) had activities that catalyzed the conversion of 9-cis-hexadecenoic acid [16:1(9c)] to 9-trans-hexadecenoic acid [16:1(9t)] in the free acid form, and when 16:1(9c) was esterified to phosphatidylethanolamine (PE). A soluble 16:1(9c)...
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ftnipr:oai:nipr.repo.nii.ac.jp:00005346 2023-05-15T18:02:00+02:00 IDENTIFICATION AND CHARACTERIZATION OF A 9-CIS-HEXADECENOIC ACID CIS-TRANS ISOMERASE FROM A PSYCHROTROPHIC BACTERIUM, PSEUDOMONAS SP. STRAIN E-3 (18th Symposium on Polar Biology) オクヤマ ヒデトシ エナリ ダイスケ モリタ ナオキ Hidetoshi OKUYAMA Daisuke ENARI Naoki MORITA 1997-02 https://nipr.repo.nii.ac.jp/?action=repository_uri&item_id=5346 http://id.nii.ac.jp/1291/00005346/ https://nipr.repo.nii.ac.jp/?action=repository_action_common_download&item_id=5346&item_no=1&attribute_id=18&file_no=1 en eng Proceeding Laboratory of Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokkaido University Division of Biological Science, Graduate School of Science, Hokkaido University:(Present address)Laboratory of Biochemistry, Hokkaido National Industrial Research Institute, AIST National Institute of Polar Research https://nipr.repo.nii.ac.jp/?action=repository_uri&item_id=5346 http://id.nii.ac.jp/1291/00005346/ AA10819561 Proceedings of the NIPR Symposium on Polar Biology, 10, 153-162(1997-02) https://nipr.repo.nii.ac.jp/?action=repository_action_common_download&item_id=5346&item_no=1&attribute_id=18&file_no=1 Departmental Bulletin Paper P(論文) 1997 ftnipr 2023-02-25T20:13:31Z A cell-free extract of Pseudomonas sp. strain E-3 (Pseudomonas E-3) had activities that catalyzed the conversion of 9-cis-hexadecenoic acid [16:1(9c)] to 9-trans-hexadecenoic acid [16:1(9t)] in the free acid form, and when 16:1(9c) was esterified to phosphatidylethanolamine (PE). A soluble 16:1(9c) cis-trans isomerase (9-Iase) was purified to complete homogeneity from the extract of Pseudomonas E-3 and characterized. Electrophoresis on both denaturing and incompletely-denaturing polyacrylamide gels of the purified enzyme preparation showed the single band of a protein with a molecular mass of 80 kDa, suggesting that the 9-Iase is a monomeric protein of 80 kDa. The 9-Iase, assayed with 16:1(9c) as a substrate, had a specific activity of 22.8 μmol per h per mg of protein and a Km of 118 μM. The enzyme had the optimum temperature for catalysis at 30℃ and catalyzed the cis to trails conversion of a double bond of 16:1(9c) in the free acid form, but it was able to isomerize 16:1(9c) esterified to PE in the presence of the cell membrane fraction. Irrespective of the temperature at which cells of Pseudomonas E-3 were grown, the level of 16:1(9t) was around 2-4% of the total cellular fatty acids. However, when cells grown at 4℃ were warmed up to 30℃ at a rate of about 20℃/min, the level of 16:1(9t) was increased from 3% to 14%. Since the level in situ of free fatty acids in this bacterium is negligible, it is suggested that the 9-Iase is operative in vivo as the cis to trans isomerase of 16:1(9c) that is esterified to PE together with the membranous factor, and that the 9-Iase might work as a stringent modulator of membrane fluidity under abrupt alteration in growth temperature. Report Polar Biology Proceedings of the NIPR Symposium on Polar Biology National Institute of Polar Research Repository, Japan |
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Open Polar |
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National Institute of Polar Research Repository, Japan |
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ftnipr |
language |
English |
description |
A cell-free extract of Pseudomonas sp. strain E-3 (Pseudomonas E-3) had activities that catalyzed the conversion of 9-cis-hexadecenoic acid [16:1(9c)] to 9-trans-hexadecenoic acid [16:1(9t)] in the free acid form, and when 16:1(9c) was esterified to phosphatidylethanolamine (PE). A soluble 16:1(9c) cis-trans isomerase (9-Iase) was purified to complete homogeneity from the extract of Pseudomonas E-3 and characterized. Electrophoresis on both denaturing and incompletely-denaturing polyacrylamide gels of the purified enzyme preparation showed the single band of a protein with a molecular mass of 80 kDa, suggesting that the 9-Iase is a monomeric protein of 80 kDa. The 9-Iase, assayed with 16:1(9c) as a substrate, had a specific activity of 22.8 μmol per h per mg of protein and a Km of 118 μM. The enzyme had the optimum temperature for catalysis at 30℃ and catalyzed the cis to trails conversion of a double bond of 16:1(9c) in the free acid form, but it was able to isomerize 16:1(9c) esterified to PE in the presence of the cell membrane fraction. Irrespective of the temperature at which cells of Pseudomonas E-3 were grown, the level of 16:1(9t) was around 2-4% of the total cellular fatty acids. However, when cells grown at 4℃ were warmed up to 30℃ at a rate of about 20℃/min, the level of 16:1(9t) was increased from 3% to 14%. Since the level in situ of free fatty acids in this bacterium is negligible, it is suggested that the 9-Iase is operative in vivo as the cis to trans isomerase of 16:1(9c) that is esterified to PE together with the membranous factor, and that the 9-Iase might work as a stringent modulator of membrane fluidity under abrupt alteration in growth temperature. |
format |
Report |
author |
オクヤマ ヒデトシ エナリ ダイスケ モリタ ナオキ Hidetoshi OKUYAMA Daisuke ENARI Naoki MORITA |
spellingShingle |
オクヤマ ヒデトシ エナリ ダイスケ モリタ ナオキ Hidetoshi OKUYAMA Daisuke ENARI Naoki MORITA IDENTIFICATION AND CHARACTERIZATION OF A 9-CIS-HEXADECENOIC ACID CIS-TRANS ISOMERASE FROM A PSYCHROTROPHIC BACTERIUM, PSEUDOMONAS SP. STRAIN E-3 (18th Symposium on Polar Biology) |
author_facet |
オクヤマ ヒデトシ エナリ ダイスケ モリタ ナオキ Hidetoshi OKUYAMA Daisuke ENARI Naoki MORITA |
author_sort |
オクヤマ ヒデトシ |
title |
IDENTIFICATION AND CHARACTERIZATION OF A 9-CIS-HEXADECENOIC ACID CIS-TRANS ISOMERASE FROM A PSYCHROTROPHIC BACTERIUM, PSEUDOMONAS SP. STRAIN E-3 (18th Symposium on Polar Biology) |
title_short |
IDENTIFICATION AND CHARACTERIZATION OF A 9-CIS-HEXADECENOIC ACID CIS-TRANS ISOMERASE FROM A PSYCHROTROPHIC BACTERIUM, PSEUDOMONAS SP. STRAIN E-3 (18th Symposium on Polar Biology) |
title_full |
IDENTIFICATION AND CHARACTERIZATION OF A 9-CIS-HEXADECENOIC ACID CIS-TRANS ISOMERASE FROM A PSYCHROTROPHIC BACTERIUM, PSEUDOMONAS SP. STRAIN E-3 (18th Symposium on Polar Biology) |
title_fullStr |
IDENTIFICATION AND CHARACTERIZATION OF A 9-CIS-HEXADECENOIC ACID CIS-TRANS ISOMERASE FROM A PSYCHROTROPHIC BACTERIUM, PSEUDOMONAS SP. STRAIN E-3 (18th Symposium on Polar Biology) |
title_full_unstemmed |
IDENTIFICATION AND CHARACTERIZATION OF A 9-CIS-HEXADECENOIC ACID CIS-TRANS ISOMERASE FROM A PSYCHROTROPHIC BACTERIUM, PSEUDOMONAS SP. STRAIN E-3 (18th Symposium on Polar Biology) |
title_sort |
identification and characterization of a 9-cis-hexadecenoic acid cis-trans isomerase from a psychrotrophic bacterium, pseudomonas sp. strain e-3 (18th symposium on polar biology) |
publisher |
Proceeding |
publishDate |
1997 |
url |
https://nipr.repo.nii.ac.jp/?action=repository_uri&item_id=5346 http://id.nii.ac.jp/1291/00005346/ https://nipr.repo.nii.ac.jp/?action=repository_action_common_download&item_id=5346&item_no=1&attribute_id=18&file_no=1 |
genre |
Polar Biology Proceedings of the NIPR Symposium on Polar Biology |
genre_facet |
Polar Biology Proceedings of the NIPR Symposium on Polar Biology |
op_relation |
https://nipr.repo.nii.ac.jp/?action=repository_uri&item_id=5346 http://id.nii.ac.jp/1291/00005346/ AA10819561 Proceedings of the NIPR Symposium on Polar Biology, 10, 153-162(1997-02) https://nipr.repo.nii.ac.jp/?action=repository_action_common_download&item_id=5346&item_no=1&attribute_id=18&file_no=1 |
_version_ |
1766171635715407872 |