THE EFFECT OF TEMPERATURE ON THE KINETIC PROPERTIES OF PHOSPHOFRUCTOKINASE AND HEXOKINASE FROM THE ANTARCTIC FISH TREMATOMUS BERNACCHII (15th Symposium on Polar Biology)
Phosphofructokinase (PFK) and hexokinase (HK) were concentrated by ammonium sulfate precipitation from the Trematomus bernacchii's epaxial and cardiac muscles respectively. The apparent Km of PFK for ATP and F6P was established at pH 8.0, at four different temperatures: 5°, 11°, 20° and 30℃. No...
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ftnipr:oai:nipr.repo.nii.ac.jp:00005222 2023-05-15T13:46:39+02:00 THE EFFECT OF TEMPERATURE ON THE KINETIC PROPERTIES OF PHOSPHOFRUCTOKINASE AND HEXOKINASE FROM THE ANTARCTIC FISH TREMATOMUS BERNACCHII (15th Symposium on Polar Biology) フクチ ミツオ ヌマナミ ヒデキ / Edson ROORIGUES Rubens ROSA Mitsuo FUKUCHI Hideki NUMANAMI Metry BACILA 1994-01 https://nipr.repo.nii.ac.jp/?action=repository_uri&item_id=5222 http://id.nii.ac.jp/1291/00005222/ https://nipr.repo.nii.ac.jp/?action=repository_action_common_download&item_id=5222&item_no=1&attribute_id=18&file_no=1 en eng Proceeding Departamento de Ciencias Fisiologicas, Faculdade de Ciencias Medicos, Universidade Sao Francisco Departamento de Bioquimica, Institute de Quimica, Universidade de Sao Paulo National Institute of Polar Research National Institute of Polar Research:(Present address)Tokyo Univrsity of Fishery Departamento de Zootecnia, Universidade Federal do Parana https://nipr.repo.nii.ac.jp/?action=repository_uri&item_id=5222 http://id.nii.ac.jp/1291/00005222/ AA10819561 Proceedings of the NIPR Symposium on Polar Biology, 7, 110-117(1994-01) https://nipr.repo.nii.ac.jp/?action=repository_action_common_download&item_id=5222&item_no=1&attribute_id=18&file_no=1 Departmental Bulletin Paper P(論文) 1994 ftnipr 2023-02-11T20:10:49Z Phosphofructokinase (PFK) and hexokinase (HK) were concentrated by ammonium sulfate precipitation from the Trematomus bernacchii's epaxial and cardiac muscles respectively. The apparent Km of PFK for ATP and F6P was established at pH 8.0, at four different temperatures: 5°, 11°, 20° and 30℃. No significant effect of the temperature on the enzyme activity was observed, due to the fact that PFK does not exhibit allosteric properties at pH 8.0. However, at pH 7.0, in which condition the allosteric properties of PFK are displayed, it was observed that the rate of inhibition of the enzyme by ATP at 5℃ is lower than at 20℃. Kinetic data of the allosteric behavior of PFK at pH 7.0, were assayed at 5° and 20℃ in the presence of fixed concentrations of ATP and variable concentrations of F6P. According to the results obtained it was possible to conclude that the enzyme affinity with the substrate was 20 times higher at 5℃ than at 20℃. This result seems to possess high significance in regard to the mechanisms of biochemical adaptation of those organisms at low temperatures. Values for energy of activation for both PFK and HK were also established. The apparent KM for HK from the cardiac muscle was equal to 384μM and 599μM at 5℃ and 20℃, respectively. Report Antarc* Antarctic Polar Biology Proceedings of the NIPR Symposium on Polar Biology National Institute of Polar Research Repository, Japan Antarctic The Antarctic |
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Open Polar |
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National Institute of Polar Research Repository, Japan |
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ftnipr |
language |
English |
description |
Phosphofructokinase (PFK) and hexokinase (HK) were concentrated by ammonium sulfate precipitation from the Trematomus bernacchii's epaxial and cardiac muscles respectively. The apparent Km of PFK for ATP and F6P was established at pH 8.0, at four different temperatures: 5°, 11°, 20° and 30℃. No significant effect of the temperature on the enzyme activity was observed, due to the fact that PFK does not exhibit allosteric properties at pH 8.0. However, at pH 7.0, in which condition the allosteric properties of PFK are displayed, it was observed that the rate of inhibition of the enzyme by ATP at 5℃ is lower than at 20℃. Kinetic data of the allosteric behavior of PFK at pH 7.0, were assayed at 5° and 20℃ in the presence of fixed concentrations of ATP and variable concentrations of F6P. According to the results obtained it was possible to conclude that the enzyme affinity with the substrate was 20 times higher at 5℃ than at 20℃. This result seems to possess high significance in regard to the mechanisms of biochemical adaptation of those organisms at low temperatures. Values for energy of activation for both PFK and HK were also established. The apparent KM for HK from the cardiac muscle was equal to 384μM and 599μM at 5℃ and 20℃, respectively. |
format |
Report |
author |
フクチ ミツオ ヌマナミ ヒデキ / Edson ROORIGUES Rubens ROSA Mitsuo FUKUCHI Hideki NUMANAMI Metry BACILA |
spellingShingle |
フクチ ミツオ ヌマナミ ヒデキ / Edson ROORIGUES Rubens ROSA Mitsuo FUKUCHI Hideki NUMANAMI Metry BACILA THE EFFECT OF TEMPERATURE ON THE KINETIC PROPERTIES OF PHOSPHOFRUCTOKINASE AND HEXOKINASE FROM THE ANTARCTIC FISH TREMATOMUS BERNACCHII (15th Symposium on Polar Biology) |
author_facet |
フクチ ミツオ ヌマナミ ヒデキ / Edson ROORIGUES Rubens ROSA Mitsuo FUKUCHI Hideki NUMANAMI Metry BACILA |
author_sort |
フクチ ミツオ |
title |
THE EFFECT OF TEMPERATURE ON THE KINETIC PROPERTIES OF PHOSPHOFRUCTOKINASE AND HEXOKINASE FROM THE ANTARCTIC FISH TREMATOMUS BERNACCHII (15th Symposium on Polar Biology) |
title_short |
THE EFFECT OF TEMPERATURE ON THE KINETIC PROPERTIES OF PHOSPHOFRUCTOKINASE AND HEXOKINASE FROM THE ANTARCTIC FISH TREMATOMUS BERNACCHII (15th Symposium on Polar Biology) |
title_full |
THE EFFECT OF TEMPERATURE ON THE KINETIC PROPERTIES OF PHOSPHOFRUCTOKINASE AND HEXOKINASE FROM THE ANTARCTIC FISH TREMATOMUS BERNACCHII (15th Symposium on Polar Biology) |
title_fullStr |
THE EFFECT OF TEMPERATURE ON THE KINETIC PROPERTIES OF PHOSPHOFRUCTOKINASE AND HEXOKINASE FROM THE ANTARCTIC FISH TREMATOMUS BERNACCHII (15th Symposium on Polar Biology) |
title_full_unstemmed |
THE EFFECT OF TEMPERATURE ON THE KINETIC PROPERTIES OF PHOSPHOFRUCTOKINASE AND HEXOKINASE FROM THE ANTARCTIC FISH TREMATOMUS BERNACCHII (15th Symposium on Polar Biology) |
title_sort |
effect of temperature on the kinetic properties of phosphofructokinase and hexokinase from the antarctic fish trematomus bernacchii (15th symposium on polar biology) |
publisher |
Proceeding |
publishDate |
1994 |
url |
https://nipr.repo.nii.ac.jp/?action=repository_uri&item_id=5222 http://id.nii.ac.jp/1291/00005222/ https://nipr.repo.nii.ac.jp/?action=repository_action_common_download&item_id=5222&item_no=1&attribute_id=18&file_no=1 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Polar Biology Proceedings of the NIPR Symposium on Polar Biology |
genre_facet |
Antarc* Antarctic Polar Biology Proceedings of the NIPR Symposium on Polar Biology |
op_relation |
https://nipr.repo.nii.ac.jp/?action=repository_uri&item_id=5222 http://id.nii.ac.jp/1291/00005222/ AA10819561 Proceedings of the NIPR Symposium on Polar Biology, 7, 110-117(1994-01) https://nipr.repo.nii.ac.jp/?action=repository_action_common_download&item_id=5222&item_no=1&attribute_id=18&file_no=1 |
_version_ |
1766244878557118464 |