Characterization of glycoprotein obtained from the skin mucus of an Antarctic fish, Trematomus bernacchii

The epithelial secretions were collected by scraping the skin of Trematomus bernacchii and seven species of temperate-water fishes and examined for the mucous glycoproteins by gel filtration on a Sepharose CL-4B column. In most cases, the glycoproteins were excluded at the void volume and separated...

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Main Authors: Makio Asakawa, Hiroki Nakagawa, Yasushi Fukuda, Mitsuo Fukuchi
Format: Report
Language:English
Published: Faculty of Education, Kumamoto University 1989
Subjects:
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spelling ftnipr:oai:nipr.repo.nii.ac.jp:00005060 2023-05-15T13:49:03+02:00 Characterization of glycoprotein obtained from the skin mucus of an Antarctic fish, Trematomus bernacchii Makio Asakawa Hiroki Nakagawa Yasushi Fukuda Mitsuo Fukuchi 1989-09 https://nipr.repo.nii.ac.jp/?action=repository_uri&item_id=5060 http://id.nii.ac.jp/1291/00005060/ https://nipr.repo.nii.ac.jp/?action=repository_action_common_download&item_id=5060&item_no=1&attribute_id=18&file_no=1 en eng Faculty of Education, Kumamoto University Department of Agricultural Chemistry,Faculty of Agriculture, Saga University National Institute of Polar Research https://nipr.repo.nii.ac.jp/?action=repository_uri&item_id=5060 http://id.nii.ac.jp/1291/00005060/ AA10819561 Proceedings of the NIPR Symposium on Polar Biology, 2, 131-138(1989-09) https://nipr.repo.nii.ac.jp/?action=repository_action_common_download&item_id=5060&item_no=1&attribute_id=18&file_no=1 Departmental Bulletin Paper P(論文) 1989 ftnipr 2023-02-11T20:10:44Z The epithelial secretions were collected by scraping the skin of Trematomus bernacchii and seven species of temperate-water fishes and examined for the mucous glycoproteins by gel filtration on a Sepharose CL-4B column. In most cases, the glycoproteins were excluded at the void volume and separated from other mucous constitutents. The yields of the glycoproteins resolved were usually high (35-45%) in the following fishes secreting large quantities of thick mucus from their epidermis: Callionymus lunatus, Misgurnus anguillicaudatus, Anguilla japonica and Leiognathus nuchalis. A fish with hard scales, Carassius auratus, gave a mucous glycoprotein in a low yield (8.4%). Similarly, the glycoproteins of T. bernacchii, Trachurus japonicus and Acanthogobius fiavimanus were obtained in their respective yields of 17.5%, 18.9%, and 14.2%. The carbohydrate composition of the mucous glycoprotein in T. bernacchii was determined to consist of N-acetylneuraminic acid, N-acetylgalactosamine, galactose, mannose, fucose and glucose by analytical methods including thin-layer chromatography, high-performance liquid chromatography and gas-liquid chromatography. The molar ratio of N-acetylneuraminic acid, N-acetylgalactosamine and galactose (1.0:4.8:1.2) suggests that major carbohydrate chains are constituted by such mono- and disaccharide units as JV-acetylgalactosamine, N-acetylneuraminyl-N-acetylgalactosamine, and galactosyl-N-acetylgalactosamine. These carbohydrate units may link through O-glycosidic linkage to threonine and serine residues in the core polypeptide. Assuming such a structure of the carbohydrate unit, it becomes probable that the mucous glycoprotein secreted from T. bernacchii epithelial tissue has a similar ability to depress freezing point for the antifreeze glycoproteins present in the serum. Report Antarc* Antarctic Polar Biology Proceedings of the NIPR Symposium on Polar Biology National Institute of Polar Research Repository, Japan Antarctic
institution Open Polar
collection National Institute of Polar Research Repository, Japan
op_collection_id ftnipr
language English
description The epithelial secretions were collected by scraping the skin of Trematomus bernacchii and seven species of temperate-water fishes and examined for the mucous glycoproteins by gel filtration on a Sepharose CL-4B column. In most cases, the glycoproteins were excluded at the void volume and separated from other mucous constitutents. The yields of the glycoproteins resolved were usually high (35-45%) in the following fishes secreting large quantities of thick mucus from their epidermis: Callionymus lunatus, Misgurnus anguillicaudatus, Anguilla japonica and Leiognathus nuchalis. A fish with hard scales, Carassius auratus, gave a mucous glycoprotein in a low yield (8.4%). Similarly, the glycoproteins of T. bernacchii, Trachurus japonicus and Acanthogobius fiavimanus were obtained in their respective yields of 17.5%, 18.9%, and 14.2%. The carbohydrate composition of the mucous glycoprotein in T. bernacchii was determined to consist of N-acetylneuraminic acid, N-acetylgalactosamine, galactose, mannose, fucose and glucose by analytical methods including thin-layer chromatography, high-performance liquid chromatography and gas-liquid chromatography. The molar ratio of N-acetylneuraminic acid, N-acetylgalactosamine and galactose (1.0:4.8:1.2) suggests that major carbohydrate chains are constituted by such mono- and disaccharide units as JV-acetylgalactosamine, N-acetylneuraminyl-N-acetylgalactosamine, and galactosyl-N-acetylgalactosamine. These carbohydrate units may link through O-glycosidic linkage to threonine and serine residues in the core polypeptide. Assuming such a structure of the carbohydrate unit, it becomes probable that the mucous glycoprotein secreted from T. bernacchii epithelial tissue has a similar ability to depress freezing point for the antifreeze glycoproteins present in the serum.
format Report
author Makio Asakawa
Hiroki Nakagawa
Yasushi Fukuda
Mitsuo Fukuchi
spellingShingle Makio Asakawa
Hiroki Nakagawa
Yasushi Fukuda
Mitsuo Fukuchi
Characterization of glycoprotein obtained from the skin mucus of an Antarctic fish, Trematomus bernacchii
author_facet Makio Asakawa
Hiroki Nakagawa
Yasushi Fukuda
Mitsuo Fukuchi
author_sort Makio Asakawa
title Characterization of glycoprotein obtained from the skin mucus of an Antarctic fish, Trematomus bernacchii
title_short Characterization of glycoprotein obtained from the skin mucus of an Antarctic fish, Trematomus bernacchii
title_full Characterization of glycoprotein obtained from the skin mucus of an Antarctic fish, Trematomus bernacchii
title_fullStr Characterization of glycoprotein obtained from the skin mucus of an Antarctic fish, Trematomus bernacchii
title_full_unstemmed Characterization of glycoprotein obtained from the skin mucus of an Antarctic fish, Trematomus bernacchii
title_sort characterization of glycoprotein obtained from the skin mucus of an antarctic fish, trematomus bernacchii
publisher Faculty of Education, Kumamoto University
publishDate 1989
url https://nipr.repo.nii.ac.jp/?action=repository_uri&item_id=5060
http://id.nii.ac.jp/1291/00005060/
https://nipr.repo.nii.ac.jp/?action=repository_action_common_download&item_id=5060&item_no=1&attribute_id=18&file_no=1
geographic Antarctic
geographic_facet Antarctic
genre Antarc*
Antarctic
Polar Biology
Proceedings of the NIPR Symposium on Polar Biology
genre_facet Antarc*
Antarctic
Polar Biology
Proceedings of the NIPR Symposium on Polar Biology
op_relation https://nipr.repo.nii.ac.jp/?action=repository_uri&item_id=5060
http://id.nii.ac.jp/1291/00005060/
AA10819561
Proceedings of the NIPR Symposium on Polar Biology, 2, 131-138(1989-09)
https://nipr.repo.nii.ac.jp/?action=repository_action_common_download&item_id=5060&item_no=1&attribute_id=18&file_no=1
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