Selective lipid recruitment by an archaeal DPANN symbiont from its host
The symbiont Ca . Nanohaloarchaeum antarcticus is obligately dependent on its host Halorubrum lacusprofundi for lipids and other metabolites due to its lack of certain biosynthetic genes. However, it remains unclear which specific lipids or metabolites are acquired from its host, and how the host re...
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Online Access: | https://www.vliz.be/imisdocs/publications/50/408250.pdf |
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ftnioz:oai:imis.nioz.nl:393662 2024-09-15T17:42:46+00:00 Selective lipid recruitment by an archaeal DPANN symbiont from its host Ding, S. Hamm, J.N. Bale, N.J. Sinninghe Damsté, J.S. Spang, A. 2024 application/pdf https://www.vliz.be/imisdocs/publications/50/408250.pdf en eng info:eu-repo/semantics/altIdentifier/doi/doi.org/10.1038/s41467-024-47750-2 https://www.vliz.be/imisdocs/publications/50/408250.pdf info:eu-repo/semantics/openAccess %3Ci%3ENature+Comm.+15%281%29%3C%2Fi%3E%3A+3405.+%3Ca+href%3D%22https%3A%2F%2Fdx.doi.org%2F10.1038%2Fs41467-024-47750-2%22+target%3D%22_blank%22%3Ehttps%3A%2F%2Fdx.doi.org%2F10.1038%2Fs41467-024-47750-2%3C%2Fa%3E info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2024 ftnioz https://doi.org/10.1038/s41467-024-47750-2 2024-08-05T23:36:41Z The symbiont Ca . Nanohaloarchaeum antarcticus is obligately dependent on its host Halorubrum lacusprofundi for lipids and other metabolites due to its lack of certain biosynthetic genes. However, it remains unclear which specific lipids or metabolites are acquired from its host, and how the host responds to infection. Here, we explored the lipidome dynamics of the Ca . Nha. antarcticus – Hrr. lacusprofundi symbiotic relationship during co-cultivation. By using a comprehensive untargeted lipidomic methodology, our study reveals that Ca . Nha. antarcticus selectively recruits 110 lipid species from its host, i.e., nearly two-thirds of the total number of host lipids. Lipid profiles of co-cultures displayed shifts in abundances of bacterioruberins and menaquinones and changes in degree of bilayer-forming glycerolipid unsaturation. This likely results in increased membrane fluidity and improved resistance to membrane disruptions, consistent with compensation for higher metabolic load and mechanical stress on host membranes when in contact with Ca . Nha. antarcticus cells. Notably, our findings differ from previous observations of other DPANN symbiont-host systems, where no differences in lipidome composition were reported. Altogether, our work emphasizes the strength of employing untargeted lipidomics approaches to provide details into the dynamics underlying a DPANN symbiont-host system. Article in Journal/Newspaper Antarc* antarcticus NIOZ Repository (Royal Netherlands Institute for Sea Research) Nature Communications 15 1 |
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Open Polar |
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NIOZ Repository (Royal Netherlands Institute for Sea Research) |
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ftnioz |
language |
English |
description |
The symbiont Ca . Nanohaloarchaeum antarcticus is obligately dependent on its host Halorubrum lacusprofundi for lipids and other metabolites due to its lack of certain biosynthetic genes. However, it remains unclear which specific lipids or metabolites are acquired from its host, and how the host responds to infection. Here, we explored the lipidome dynamics of the Ca . Nha. antarcticus – Hrr. lacusprofundi symbiotic relationship during co-cultivation. By using a comprehensive untargeted lipidomic methodology, our study reveals that Ca . Nha. antarcticus selectively recruits 110 lipid species from its host, i.e., nearly two-thirds of the total number of host lipids. Lipid profiles of co-cultures displayed shifts in abundances of bacterioruberins and menaquinones and changes in degree of bilayer-forming glycerolipid unsaturation. This likely results in increased membrane fluidity and improved resistance to membrane disruptions, consistent with compensation for higher metabolic load and mechanical stress on host membranes when in contact with Ca . Nha. antarcticus cells. Notably, our findings differ from previous observations of other DPANN symbiont-host systems, where no differences in lipidome composition were reported. Altogether, our work emphasizes the strength of employing untargeted lipidomics approaches to provide details into the dynamics underlying a DPANN symbiont-host system. |
format |
Article in Journal/Newspaper |
author |
Ding, S. Hamm, J.N. Bale, N.J. Sinninghe Damsté, J.S. Spang, A. |
spellingShingle |
Ding, S. Hamm, J.N. Bale, N.J. Sinninghe Damsté, J.S. Spang, A. Selective lipid recruitment by an archaeal DPANN symbiont from its host |
author_facet |
Ding, S. Hamm, J.N. Bale, N.J. Sinninghe Damsté, J.S. Spang, A. |
author_sort |
Ding, S. |
title |
Selective lipid recruitment by an archaeal DPANN symbiont from its host |
title_short |
Selective lipid recruitment by an archaeal DPANN symbiont from its host |
title_full |
Selective lipid recruitment by an archaeal DPANN symbiont from its host |
title_fullStr |
Selective lipid recruitment by an archaeal DPANN symbiont from its host |
title_full_unstemmed |
Selective lipid recruitment by an archaeal DPANN symbiont from its host |
title_sort |
selective lipid recruitment by an archaeal dpann symbiont from its host |
publishDate |
2024 |
url |
https://www.vliz.be/imisdocs/publications/50/408250.pdf |
genre |
Antarc* antarcticus |
genre_facet |
Antarc* antarcticus |
op_source |
%3Ci%3ENature+Comm.+15%281%29%3C%2Fi%3E%3A+3405.+%3Ca+href%3D%22https%3A%2F%2Fdx.doi.org%2F10.1038%2Fs41467-024-47750-2%22+target%3D%22_blank%22%3Ehttps%3A%2F%2Fdx.doi.org%2F10.1038%2Fs41467-024-47750-2%3C%2Fa%3E |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/doi.org/10.1038/s41467-024-47750-2 https://www.vliz.be/imisdocs/publications/50/408250.pdf |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1038/s41467-024-47750-2 |
container_title |
Nature Communications |
container_volume |
15 |
container_issue |
1 |
_version_ |
1810489502432493568 |