A device for assessing microbial activity under ambient hydrostatic pressure: The in situ microbial incubator (<scp>ISMI</scp>)
Microbes in the dark ocean are exposed to hydrostatic pressure increasing with depth. Activity rate measurements and biomass production of dark ocean microbes are, however, almost exclusively performed under atmospheric pressure conditions due to technical constraints of sampling equipment maintaini...
Published in: | Limnology and Oceanography: Methods |
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Language: | English |
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Online Access: | https://www.vliz.be/imisdocs/publications/02/387102.pdf |
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ftnioz:oai:imis.nioz.nl:360146 2023-05-15T17:34:07+02:00 A device for assessing microbial activity under ambient hydrostatic pressure: The in situ microbial incubator (<scp>ISMI</scp>) Amano, C. Reinthaler, T. Sintes, E. Varela, M.M. Stefanschitz, J. Kaneko, S. Nakano, Y. Borchert, W. Herndl, G.J. Utsumi, M. 2023 application/pdf https://www.vliz.be/imisdocs/publications/02/387102.pdf en eng info:eu-repo/semantics/altIdentifier/wos/000898139300001 info:eu-repo/semantics/altIdentifier/doi/doi.org/10.1002/lom3.10528 https://www.vliz.be/imisdocs/publications/02/387102.pdf info:eu-repo/semantics/openAccess %3Ci%3ELimnol.+Oceanogr.,+Methods+21%282%29%3C%2Fi%3E%3A+69-81.+%3Ca+href%3D%22https%3A%2F%2Fdx.doi.org%2F10.1002%2Flom3.10528%22+target%3D%22_blank%22%3Ehttps%3A%2F%2Fdx.doi.org%2F10.1002%2Flom3.10528%3C%2Fa%3E info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2023 ftnioz https://doi.org/10.1002/lom3.10528 2023-03-01T23:20:16Z Microbes in the dark ocean are exposed to hydrostatic pressure increasing with depth. Activity rate measurements and biomass production of dark ocean microbes are, however, almost exclusively performed under atmospheric pressure conditions due to technical constraints of sampling equipment maintaining in situ pressure conditions. To evaluate the microbial activity under in situ hydrostatic pressure, we designed and thoroughly tested an in situ microbial incubator (ISMI). The ISMI allows autonomously collecting and incubating seawater at depth, injection of substrate and fixation of the samples after a preprogramed incubation time. The performance of the ISMI was tested in a high-pressure tank and in several field campaigns under ambient hydrostatic pressure by measuring prokaryotic bulk 3H-leucine incorporation rates. Overall, prokaryotic leucine incorporation rates were lower at in situ pressure conditions than under to depressurized conditions reaching only about 50% of the heterotrophic microbial activity measured under depressurized conditions in bathypelagic waters in the North Atlantic Ocean off the northwestern Iberian Peninsula. Our results show that the ISMI is a valuable tool to reliably determine the metabolic activity of deep-sea microbes at in situ hydrostatic pressure conditions. Hence, we advocate that deep-sea biogeochemical and microbial rate measurements should be performed under in situ pressure conditions to obtain a more realistic view on deep-sea biotic processes. Article in Journal/Newspaper North Atlantic NIOZ Repository (Royal Netherlands Institute for Sea Research) Limnology and Oceanography: Methods |
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NIOZ Repository (Royal Netherlands Institute for Sea Research) |
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ftnioz |
language |
English |
description |
Microbes in the dark ocean are exposed to hydrostatic pressure increasing with depth. Activity rate measurements and biomass production of dark ocean microbes are, however, almost exclusively performed under atmospheric pressure conditions due to technical constraints of sampling equipment maintaining in situ pressure conditions. To evaluate the microbial activity under in situ hydrostatic pressure, we designed and thoroughly tested an in situ microbial incubator (ISMI). The ISMI allows autonomously collecting and incubating seawater at depth, injection of substrate and fixation of the samples after a preprogramed incubation time. The performance of the ISMI was tested in a high-pressure tank and in several field campaigns under ambient hydrostatic pressure by measuring prokaryotic bulk 3H-leucine incorporation rates. Overall, prokaryotic leucine incorporation rates were lower at in situ pressure conditions than under to depressurized conditions reaching only about 50% of the heterotrophic microbial activity measured under depressurized conditions in bathypelagic waters in the North Atlantic Ocean off the northwestern Iberian Peninsula. Our results show that the ISMI is a valuable tool to reliably determine the metabolic activity of deep-sea microbes at in situ hydrostatic pressure conditions. Hence, we advocate that deep-sea biogeochemical and microbial rate measurements should be performed under in situ pressure conditions to obtain a more realistic view on deep-sea biotic processes. |
format |
Article in Journal/Newspaper |
author |
Amano, C. Reinthaler, T. Sintes, E. Varela, M.M. Stefanschitz, J. Kaneko, S. Nakano, Y. Borchert, W. Herndl, G.J. Utsumi, M. |
spellingShingle |
Amano, C. Reinthaler, T. Sintes, E. Varela, M.M. Stefanschitz, J. Kaneko, S. Nakano, Y. Borchert, W. Herndl, G.J. Utsumi, M. A device for assessing microbial activity under ambient hydrostatic pressure: The in situ microbial incubator (<scp>ISMI</scp>) |
author_facet |
Amano, C. Reinthaler, T. Sintes, E. Varela, M.M. Stefanschitz, J. Kaneko, S. Nakano, Y. Borchert, W. Herndl, G.J. Utsumi, M. |
author_sort |
Amano, C. |
title |
A device for assessing microbial activity under ambient hydrostatic pressure: The in situ microbial incubator (<scp>ISMI</scp>) |
title_short |
A device for assessing microbial activity under ambient hydrostatic pressure: The in situ microbial incubator (<scp>ISMI</scp>) |
title_full |
A device for assessing microbial activity under ambient hydrostatic pressure: The in situ microbial incubator (<scp>ISMI</scp>) |
title_fullStr |
A device for assessing microbial activity under ambient hydrostatic pressure: The in situ microbial incubator (<scp>ISMI</scp>) |
title_full_unstemmed |
A device for assessing microbial activity under ambient hydrostatic pressure: The in situ microbial incubator (<scp>ISMI</scp>) |
title_sort |
device for assessing microbial activity under ambient hydrostatic pressure: the in situ microbial incubator (<scp>ismi</scp>) |
publishDate |
2023 |
url |
https://www.vliz.be/imisdocs/publications/02/387102.pdf |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
%3Ci%3ELimnol.+Oceanogr.,+Methods+21%282%29%3C%2Fi%3E%3A+69-81.+%3Ca+href%3D%22https%3A%2F%2Fdx.doi.org%2F10.1002%2Flom3.10528%22+target%3D%22_blank%22%3Ehttps%3A%2F%2Fdx.doi.org%2F10.1002%2Flom3.10528%3C%2Fa%3E |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/000898139300001 info:eu-repo/semantics/altIdentifier/doi/doi.org/10.1002/lom3.10528 https://www.vliz.be/imisdocs/publications/02/387102.pdf |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1002/lom3.10528 |
container_title |
Limnology and Oceanography: Methods |
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1766132843495292928 |