Mixotrophy in the bloom-forming genus Phaeocystis and other haptophytes
Phaeocystis is a globally widespread marine phytoplankton genus, best known for its colony-forming species that can form large blooms and odorous foam during bloom decline. In the North Sea, Phaeocystis globosa typically becomes abundant towards the end of the spring bloom, when nutrients are deplet...
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ftnioz:oai:imis.nioz.nl:354614 2023-05-15T13:47:57+02:00 Mixotrophy in the bloom-forming genus Phaeocystis and other haptophytes Koppelle, S. López-Escardó, D. Brussaard, C.P.D. Huisman, J. Philippart, C.J.M. Massana, R. Wilken, S. 2022 application/pdf https://www.vliz.be/imisdocs/publications/95/380495.pdf en eng info:eu-repo/semantics/altIdentifier/doi/doi.org/10.1016/j.hal.2022.102292 https://www.vliz.be/imisdocs/publications/95/380495.pdf info:eu-repo/semantics/openAccess %3Ci%3EHarmful+Algae+117%3C%2Fi%3E%3A+102292.+%3Ca+href%3D%22https%3A%2F%2Fdx.doi.org%2F10.1016%2Fj.hal.2022.102292%22+target%3D%22_blank%22%3Ehttps%3A%2F%2Fdx.doi.org%2F10.1016%2Fj.hal.2022.102292%3C%2Fa%3E Phaeocystis globosa info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2022 ftnioz https://doi.org/10.1016/j.hal.2022.102292 2022-10-05T22:19:47Z Phaeocystis is a globally widespread marine phytoplankton genus, best known for its colony-forming species that can form large blooms and odorous foam during bloom decline. In the North Sea, Phaeocystis globosa typically becomes abundant towards the end of the spring bloom, when nutrients are depleted and the share of mixotrophic protists increases. Although mixotrophy is widespread across the eukaryotic tree of life and is also found amongst haptophytes, a mixotrophic nutrition has not yet beendemonstrated in Phaeocystis . Here, we sampled two consecutive Phaeocystis globosa spring blooms in the coastal North Sea. In both years, bacterial cells were observed inside 0.6 – 2% of P. globosa cells using double CARD-FISH hybridizations in combination with laser scanning confocal microscopy. Incubation experiments manipulating light and nutrient availability showed a trend towards higher occurrence of intracellular bacteria under P-deplete conditions. Based on counts of bacteria inside P. globosa cells in combination with theoretical values of prey digestion times, maximum ingestion rates of up to 0.08 bacteria cell −1 h −1 were estimated. In addition, a gene-based predictive model was applied to the transcriptome assemblies of seven Phaeocystis strains and 24 other haptophytes to assess their trophic mode. This model predicted a phago-mixotrophic feeding strategy in several (but not all) strains of P. globosa, P. antarctica and other haptophytes that were previously assumed to be autotrophic. The observation of bacterial cells inside P. globosa and the gene-based model predictions strongly suggest that the phago-mixotrophic feeding strategy is widespread among members of the Phaeocystis genus and other haptophytes, and might contribute to their remarkable success to form nuisance blooms under nutrient-limiting conditions. Article in Journal/Newspaper Antarc* Antarctica NIOZ Repository (Royal Netherlands Institute for Sea Research) Harmful Algae 117 102292 |
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Open Polar |
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NIOZ Repository (Royal Netherlands Institute for Sea Research) |
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ftnioz |
language |
English |
topic |
Phaeocystis globosa |
spellingShingle |
Phaeocystis globosa Koppelle, S. López-Escardó, D. Brussaard, C.P.D. Huisman, J. Philippart, C.J.M. Massana, R. Wilken, S. Mixotrophy in the bloom-forming genus Phaeocystis and other haptophytes |
topic_facet |
Phaeocystis globosa |
description |
Phaeocystis is a globally widespread marine phytoplankton genus, best known for its colony-forming species that can form large blooms and odorous foam during bloom decline. In the North Sea, Phaeocystis globosa typically becomes abundant towards the end of the spring bloom, when nutrients are depleted and the share of mixotrophic protists increases. Although mixotrophy is widespread across the eukaryotic tree of life and is also found amongst haptophytes, a mixotrophic nutrition has not yet beendemonstrated in Phaeocystis . Here, we sampled two consecutive Phaeocystis globosa spring blooms in the coastal North Sea. In both years, bacterial cells were observed inside 0.6 – 2% of P. globosa cells using double CARD-FISH hybridizations in combination with laser scanning confocal microscopy. Incubation experiments manipulating light and nutrient availability showed a trend towards higher occurrence of intracellular bacteria under P-deplete conditions. Based on counts of bacteria inside P. globosa cells in combination with theoretical values of prey digestion times, maximum ingestion rates of up to 0.08 bacteria cell −1 h −1 were estimated. In addition, a gene-based predictive model was applied to the transcriptome assemblies of seven Phaeocystis strains and 24 other haptophytes to assess their trophic mode. This model predicted a phago-mixotrophic feeding strategy in several (but not all) strains of P. globosa, P. antarctica and other haptophytes that were previously assumed to be autotrophic. The observation of bacterial cells inside P. globosa and the gene-based model predictions strongly suggest that the phago-mixotrophic feeding strategy is widespread among members of the Phaeocystis genus and other haptophytes, and might contribute to their remarkable success to form nuisance blooms under nutrient-limiting conditions. |
format |
Article in Journal/Newspaper |
author |
Koppelle, S. López-Escardó, D. Brussaard, C.P.D. Huisman, J. Philippart, C.J.M. Massana, R. Wilken, S. |
author_facet |
Koppelle, S. López-Escardó, D. Brussaard, C.P.D. Huisman, J. Philippart, C.J.M. Massana, R. Wilken, S. |
author_sort |
Koppelle, S. |
title |
Mixotrophy in the bloom-forming genus Phaeocystis and other haptophytes |
title_short |
Mixotrophy in the bloom-forming genus Phaeocystis and other haptophytes |
title_full |
Mixotrophy in the bloom-forming genus Phaeocystis and other haptophytes |
title_fullStr |
Mixotrophy in the bloom-forming genus Phaeocystis and other haptophytes |
title_full_unstemmed |
Mixotrophy in the bloom-forming genus Phaeocystis and other haptophytes |
title_sort |
mixotrophy in the bloom-forming genus phaeocystis and other haptophytes |
publishDate |
2022 |
url |
https://www.vliz.be/imisdocs/publications/95/380495.pdf |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
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op_relation |
info:eu-repo/semantics/altIdentifier/doi/doi.org/10.1016/j.hal.2022.102292 https://www.vliz.be/imisdocs/publications/95/380495.pdf |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1016/j.hal.2022.102292 |
container_title |
Harmful Algae |
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117 |
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102292 |
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