Hydrolytic capabilities as a key to environmental success: chitinolytic and cellulolytic Acidobacteria from acidic sub-arctic soils and boreal peatlands
Members of the Acidobacteria are among the most efficient colonizers of acidic terrestrial habitats but the key traits underlying their environmental fitness remain to be understood. We analyzed indigenous assemblages of Acidobacteria in a lichen-covered acidic (pH 4.1) soil of forested tundra domin...
Published in: | Frontiers in Microbiology |
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Language: | English |
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2018
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Online Access: | https://www.vliz.be/imisdocs/publications/57/321457.pdf |
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ftnioz:oai:imis.nioz.nl:303433 2023-05-15T15:07:21+02:00 Hydrolytic capabilities as a key to environmental success: chitinolytic and cellulolytic Acidobacteria from acidic sub-arctic soils and boreal peatlands Belova, S.E. Ravin, N.V. Pankratov, T.A. Rakitin, A.L. Ivanova, A. Beletsky, A.V. Mardanov, A.V. Sinninghe Damsté, J.S. Dedysh, S.N. 2018 application/pdf https://www.vliz.be/imisdocs/publications/57/321457.pdf en eng info:eu-repo/semantics/altIdentifier/wos/000450438400001 info:eu-repo/semantics/altIdentifier/doi/.org/10.3389/fmicb.2018.02775 https://www.vliz.be/imisdocs/publications/57/321457.pdf info:eu-repo/semantics/openAccess %3Ci%3EFront.+Microbiol.+9%3C%2Fi%3E%3A+2775.+%3Ca+href%3D%22https%3A%2F%2Fdoi.org%2F10.3389%2Ffmicb.2018.02775%22+target%3D%22_blank%22%3Ehttps%3A%2F%2Fdoi.org%2F10.3389%2Ffmicb.2018.02775%3C%2Fa%3E Acidisarcina Acidobacteria info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2018 ftnioz https://doi.org/10.3389/fmicb.2018.02775 2022-05-01T14:08:31Z Members of the Acidobacteria are among the most efficient colonizers of acidic terrestrial habitats but the key traits underlying their environmental fitness remain to be understood. We analyzed indigenous assemblages of Acidobacteria in a lichen-covered acidic (pH 4.1) soil of forested tundra dominated by uncultivated members of subdivision 1. An isolate of these bacteria with cells occurring within saccular chambers, strain SBC82 T , was obtained. The genome of strain SBC82 T consists of a 7.11-Mb chromosome and four megaplasmids, and encodes a wide repertoire of enzymes involved in degradation of chitin, cellulose, and xylan. Among those, four secreted chitinases affiliated with the glycoside hydrolase family GH18 were identified. Strain SBC82T utilized amorphous chitin as a source of carbon and nitrogen; the respective enzyme activities were detected in tests with synthetic substrates. Chitinolytic capability was also confirmed for another phylogenetically related acidobacterium isolated from a Sphagnum peat bog, strain CCO287. As revealed by metatranscriptomic analysis of chitin-amended peat, 16S rRNA reads from these acidobacteria increased in response to chitin availability. Strains SBC82 T and CCO287 were assigned to a novel genus and species, Acidisarcina polymorpha en. nov., sp. nov. Members of this genus colonize acidic soils and peatlands and specialize in degrading complex polysaccharides. Article in Journal/Newspaper Arctic Tundra NIOZ Repository (Royal Netherlands Institute for Sea Research) Arctic Frontiers in Microbiology 9 |
institution |
Open Polar |
collection |
NIOZ Repository (Royal Netherlands Institute for Sea Research) |
op_collection_id |
ftnioz |
language |
English |
topic |
Acidisarcina Acidobacteria |
spellingShingle |
Acidisarcina Acidobacteria Belova, S.E. Ravin, N.V. Pankratov, T.A. Rakitin, A.L. Ivanova, A. Beletsky, A.V. Mardanov, A.V. Sinninghe Damsté, J.S. Dedysh, S.N. Hydrolytic capabilities as a key to environmental success: chitinolytic and cellulolytic Acidobacteria from acidic sub-arctic soils and boreal peatlands |
topic_facet |
Acidisarcina Acidobacteria |
description |
Members of the Acidobacteria are among the most efficient colonizers of acidic terrestrial habitats but the key traits underlying their environmental fitness remain to be understood. We analyzed indigenous assemblages of Acidobacteria in a lichen-covered acidic (pH 4.1) soil of forested tundra dominated by uncultivated members of subdivision 1. An isolate of these bacteria with cells occurring within saccular chambers, strain SBC82 T , was obtained. The genome of strain SBC82 T consists of a 7.11-Mb chromosome and four megaplasmids, and encodes a wide repertoire of enzymes involved in degradation of chitin, cellulose, and xylan. Among those, four secreted chitinases affiliated with the glycoside hydrolase family GH18 were identified. Strain SBC82T utilized amorphous chitin as a source of carbon and nitrogen; the respective enzyme activities were detected in tests with synthetic substrates. Chitinolytic capability was also confirmed for another phylogenetically related acidobacterium isolated from a Sphagnum peat bog, strain CCO287. As revealed by metatranscriptomic analysis of chitin-amended peat, 16S rRNA reads from these acidobacteria increased in response to chitin availability. Strains SBC82 T and CCO287 were assigned to a novel genus and species, Acidisarcina polymorpha en. nov., sp. nov. Members of this genus colonize acidic soils and peatlands and specialize in degrading complex polysaccharides. |
format |
Article in Journal/Newspaper |
author |
Belova, S.E. Ravin, N.V. Pankratov, T.A. Rakitin, A.L. Ivanova, A. Beletsky, A.V. Mardanov, A.V. Sinninghe Damsté, J.S. Dedysh, S.N. |
author_facet |
Belova, S.E. Ravin, N.V. Pankratov, T.A. Rakitin, A.L. Ivanova, A. Beletsky, A.V. Mardanov, A.V. Sinninghe Damsté, J.S. Dedysh, S.N. |
author_sort |
Belova, S.E. |
title |
Hydrolytic capabilities as a key to environmental success: chitinolytic and cellulolytic Acidobacteria from acidic sub-arctic soils and boreal peatlands |
title_short |
Hydrolytic capabilities as a key to environmental success: chitinolytic and cellulolytic Acidobacteria from acidic sub-arctic soils and boreal peatlands |
title_full |
Hydrolytic capabilities as a key to environmental success: chitinolytic and cellulolytic Acidobacteria from acidic sub-arctic soils and boreal peatlands |
title_fullStr |
Hydrolytic capabilities as a key to environmental success: chitinolytic and cellulolytic Acidobacteria from acidic sub-arctic soils and boreal peatlands |
title_full_unstemmed |
Hydrolytic capabilities as a key to environmental success: chitinolytic and cellulolytic Acidobacteria from acidic sub-arctic soils and boreal peatlands |
title_sort |
hydrolytic capabilities as a key to environmental success: chitinolytic and cellulolytic acidobacteria from acidic sub-arctic soils and boreal peatlands |
publishDate |
2018 |
url |
https://www.vliz.be/imisdocs/publications/57/321457.pdf |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Tundra |
genre_facet |
Arctic Tundra |
op_source |
%3Ci%3EFront.+Microbiol.+9%3C%2Fi%3E%3A+2775.+%3Ca+href%3D%22https%3A%2F%2Fdoi.org%2F10.3389%2Ffmicb.2018.02775%22+target%3D%22_blank%22%3Ehttps%3A%2F%2Fdoi.org%2F10.3389%2Ffmicb.2018.02775%3C%2Fa%3E |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/000450438400001 info:eu-repo/semantics/altIdentifier/doi/.org/10.3389/fmicb.2018.02775 https://www.vliz.be/imisdocs/publications/57/321457.pdf |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.3389/fmicb.2018.02775 |
container_title |
Frontiers in Microbiology |
container_volume |
9 |
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1766338870502227968 |