Plasma proteomics data from hibernating and active Scandinavian brown bears
In this article, we present mass-spectrometry based plasma proteomics data from hibernating and active free-ranging Scandinavian brown bears (Ursus arctos). The brown bear hibernates for half the year. Despite obesity when entering the den and the prolonged period of inactiv- ity, the bear shows no...
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ftninstnf:oai:brage.nina.no:11250/3010128 2023-05-15T18:41:57+02:00 Plasma proteomics data from hibernating and active Scandinavian brown bears Fröbert, Anne Mette Gregersen, Simon Brohus, Malene Welinder, Karen G. Kindberg, Jonas Fröbert, Ole Overgaard, Michael T. Skandinavia, Scandinavia 2022 application/pdf https://hdl.handle.net/11250/3010128 https://doi.org/10.1016/j.dib.2022.107959 eng eng Andre: Lundbeck Foundation [286–2018–367 ] Andre: Lundbeck Foundation [ R126–2012–12,408 ] urn:issn:2352-3409 https://hdl.handle.net/11250/3010128 https://doi.org/10.1016/j.dib.2022.107959 cristin:2004049 Navngivelse 4.0 Internasjonal http://creativecommons.org/licenses/by/4.0/deed.no © 2022 The Authors CC-BY 41 Data in Brief 107959 VDP::Zoologiske og botaniske fag: 480 VDP::Zoology and botany: 480 Peer reviewed Journal article 2022 ftninstnf https://doi.org/10.1016/j.dib.2022.107959 2022-08-10T22:43:18Z In this article, we present mass-spectrometry based plasma proteomics data from hibernating and active free-ranging Scandinavian brown bears (Ursus arctos). The brown bear hibernates for half the year. Despite obesity when entering the den and the prolonged period of inactiv- ity, the bear shows no signs of the harmful effects associated with these conditions in humans. Thus, the hibernating bear is a potential translational model for addressing these com- plications in humans. We analyzed plasma samples from fourteen 2- to 3-year-old bears (6 males and 8 females) collected both during hiberna- tion and the active state, and for some of the bears during two seasons, resulting in a total of 38 analyzed plasma sam- ples. In triplicates, the plasma proteins were unfolded and re- duced. To increase the chance of detecting low-molecular- weight proteins and peptides, we filtered the samples using a 50 K molecular weight cut-offfilter with the aim to depletelarger abundant proteins, including albumin, and thereby in- crease the depth of the analysis. The proteins in the per- meate were then tryptically digested, desalted, and analyzed with liquid chromatography-tandem mass spectrometry (LC- MS/MS). Protein identification and quantification was per- formed with the MaxQuant software searching against an Ur- sus arctos horribilis protein database. Here, we provide the raw data, a list with identified proteins in the plasma samples, and the databases applied for protein identification. Based on the provided data, differentially expressed proteins in hibernation compared to active state can be identified. These proteins may be involved in the bears’ adaptions to hi- bernation physiology and hold potential as novel therapeutic targets. Ursus arctos Hibernation Proteins Blood Mass spectrometry Translational medicine publishedVersion Article in Journal/Newspaper Ursus arctos Norwegian Institute for Nature Research: Brage NINA Data in Brief 41 107959 |
institution |
Open Polar |
collection |
Norwegian Institute for Nature Research: Brage NINA |
op_collection_id |
ftninstnf |
language |
English |
topic |
VDP::Zoologiske og botaniske fag: 480 VDP::Zoology and botany: 480 |
spellingShingle |
VDP::Zoologiske og botaniske fag: 480 VDP::Zoology and botany: 480 Fröbert, Anne Mette Gregersen, Simon Brohus, Malene Welinder, Karen G. Kindberg, Jonas Fröbert, Ole Overgaard, Michael T. Plasma proteomics data from hibernating and active Scandinavian brown bears |
topic_facet |
VDP::Zoologiske og botaniske fag: 480 VDP::Zoology and botany: 480 |
description |
In this article, we present mass-spectrometry based plasma proteomics data from hibernating and active free-ranging Scandinavian brown bears (Ursus arctos). The brown bear hibernates for half the year. Despite obesity when entering the den and the prolonged period of inactiv- ity, the bear shows no signs of the harmful effects associated with these conditions in humans. Thus, the hibernating bear is a potential translational model for addressing these com- plications in humans. We analyzed plasma samples from fourteen 2- to 3-year-old bears (6 males and 8 females) collected both during hiberna- tion and the active state, and for some of the bears during two seasons, resulting in a total of 38 analyzed plasma sam- ples. In triplicates, the plasma proteins were unfolded and re- duced. To increase the chance of detecting low-molecular- weight proteins and peptides, we filtered the samples using a 50 K molecular weight cut-offfilter with the aim to depletelarger abundant proteins, including albumin, and thereby in- crease the depth of the analysis. The proteins in the per- meate were then tryptically digested, desalted, and analyzed with liquid chromatography-tandem mass spectrometry (LC- MS/MS). Protein identification and quantification was per- formed with the MaxQuant software searching against an Ur- sus arctos horribilis protein database. Here, we provide the raw data, a list with identified proteins in the plasma samples, and the databases applied for protein identification. Based on the provided data, differentially expressed proteins in hibernation compared to active state can be identified. These proteins may be involved in the bears’ adaptions to hi- bernation physiology and hold potential as novel therapeutic targets. Ursus arctos Hibernation Proteins Blood Mass spectrometry Translational medicine publishedVersion |
format |
Article in Journal/Newspaper |
author |
Fröbert, Anne Mette Gregersen, Simon Brohus, Malene Welinder, Karen G. Kindberg, Jonas Fröbert, Ole Overgaard, Michael T. |
author_facet |
Fröbert, Anne Mette Gregersen, Simon Brohus, Malene Welinder, Karen G. Kindberg, Jonas Fröbert, Ole Overgaard, Michael T. |
author_sort |
Fröbert, Anne Mette |
title |
Plasma proteomics data from hibernating and active Scandinavian brown bears |
title_short |
Plasma proteomics data from hibernating and active Scandinavian brown bears |
title_full |
Plasma proteomics data from hibernating and active Scandinavian brown bears |
title_fullStr |
Plasma proteomics data from hibernating and active Scandinavian brown bears |
title_full_unstemmed |
Plasma proteomics data from hibernating and active Scandinavian brown bears |
title_sort |
plasma proteomics data from hibernating and active scandinavian brown bears |
publishDate |
2022 |
url |
https://hdl.handle.net/11250/3010128 https://doi.org/10.1016/j.dib.2022.107959 |
op_coverage |
Skandinavia, Scandinavia |
genre |
Ursus arctos |
genre_facet |
Ursus arctos |
op_source |
41 Data in Brief 107959 |
op_relation |
Andre: Lundbeck Foundation [286–2018–367 ] Andre: Lundbeck Foundation [ R126–2012–12,408 ] urn:issn:2352-3409 https://hdl.handle.net/11250/3010128 https://doi.org/10.1016/j.dib.2022.107959 cristin:2004049 |
op_rights |
Navngivelse 4.0 Internasjonal http://creativecommons.org/licenses/by/4.0/deed.no © 2022 The Authors |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1016/j.dib.2022.107959 |
container_title |
Data in Brief |
container_volume |
41 |
container_start_page |
107959 |
_version_ |
1766231534661009408 |