Using genomics to guide seed-sourcing at the right taxonomical level for ecological restoration projects: The complex case of Carex bigelowii s.lat. in Norway
There is a growing demand for ecological restoration using suitable seeds following international standards or national legal demands for local seed-sourcing. However, before selecting the appropriate geographic origin of seeds, it is vital to explore taxonomic complexity related to the focal taxa....
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Online Access: | https://hdl.handle.net/11250/2986529 https://doi.org/10.1002/ece3.8350 |
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ftninstnf:oai:brage.nina.no:11250/2986529 2023-05-15T15:53:17+02:00 Using genomics to guide seed-sourcing at the right taxonomical level for ecological restoration projects: The complex case of Carex bigelowii s.lat. in Norway Westergaard, Kristine Bakke Kyrkjeeide, Magni Olsen Brandrud, Marie Kristine Norway, Norge, Noreg 2021 application/pdf https://hdl.handle.net/11250/2986529 https://doi.org/10.1002/ece3.8350 eng eng Norges forskningsråd: 160022 urn:issn:2045-7758 https://hdl.handle.net/11250/2986529 https://doi.org/10.1002/ece3.8350 cristin:1957412 Navngivelse 4.0 Internasjonal http://creativecommons.org/licenses/by/4.0/deed.no © 2021 The Authors CC-BY Ecology and Evolution alpine ecological restoration Carex bigelowii s.lat ddRAD-seq genetic structure hybrids seed-sourcing subspecies taxonomic VDP::Zoologiske og botaniske fag: 480 VDP::Zoology and botany: 480 Peer reviewed Journal article 2021 ftninstnf https://doi.org/10.1002/ece3.8350 2022-03-23T23:41:22Z There is a growing demand for ecological restoration using suitable seeds following international standards or national legal demands for local seed-sourcing. However, before selecting the appropriate geographic origin of seeds, it is vital to explore taxonomic complexity related to the focal taxa. We used ddRAD-seq to screen genomic diversity within Carex bigelowii s.lat. focussing on Norway. This species complex is considered a candidate for seeding, but presents considerable morphological, ecological, and genetic variation. The genetic structure of 132 individuals of C. bigelowii s.lat., including Carex nigra as an outgroup, was explored using ordinations, clustering analyses, and a genetic barrier algorithm. Two highly divergent clusters were evident, supporting the recognition of two taxonomic units “C. dacica” and C. bigelowii “subsp. bigelowii”. Previously defined seed-sourcing regions for C. bigelowii s.lat. did not consider the known taxonomic complexity, and therefore interpreted the overall genetic structure as seed-sourcing regions, not taxa. We estimated genetic neighborhood sizes within each taxon to be 100–150 km and 300 km, respectively, indicating species-specific delimitations of local seed-sourcing regions. Frequent hybrids, local genetic distinctiveness, and suggested ecotypes add complexity to the discussed seed-sourcing regions. Our results show how genomic screening of diversity and structure in a species complex can alleviate the taxonomic impediment, inform practical questions, and legal requirements related to seed-sourcing, and together with traditional taxonomic work provide necessary information for a sound management of biodiversity. alpine ecological restoration, Carex bigelowii s.lat., ddRAD-seq, genetic structure, hybrids, seed-sourcing, subspecies, taxonomic impediment publishedVersion Article in Journal/Newspaper Carex bigelowii Norwegian Institute for Nature Research: Brage NINA Norway Ecology and Evolution 11 23 17117 17131 |
institution |
Open Polar |
collection |
Norwegian Institute for Nature Research: Brage NINA |
op_collection_id |
ftninstnf |
language |
English |
topic |
alpine ecological restoration Carex bigelowii s.lat ddRAD-seq genetic structure hybrids seed-sourcing subspecies taxonomic VDP::Zoologiske og botaniske fag: 480 VDP::Zoology and botany: 480 |
spellingShingle |
alpine ecological restoration Carex bigelowii s.lat ddRAD-seq genetic structure hybrids seed-sourcing subspecies taxonomic VDP::Zoologiske og botaniske fag: 480 VDP::Zoology and botany: 480 Westergaard, Kristine Bakke Kyrkjeeide, Magni Olsen Brandrud, Marie Kristine Using genomics to guide seed-sourcing at the right taxonomical level for ecological restoration projects: The complex case of Carex bigelowii s.lat. in Norway |
topic_facet |
alpine ecological restoration Carex bigelowii s.lat ddRAD-seq genetic structure hybrids seed-sourcing subspecies taxonomic VDP::Zoologiske og botaniske fag: 480 VDP::Zoology and botany: 480 |
description |
There is a growing demand for ecological restoration using suitable seeds following international standards or national legal demands for local seed-sourcing. However, before selecting the appropriate geographic origin of seeds, it is vital to explore taxonomic complexity related to the focal taxa. We used ddRAD-seq to screen genomic diversity within Carex bigelowii s.lat. focussing on Norway. This species complex is considered a candidate for seeding, but presents considerable morphological, ecological, and genetic variation. The genetic structure of 132 individuals of C. bigelowii s.lat., including Carex nigra as an outgroup, was explored using ordinations, clustering analyses, and a genetic barrier algorithm. Two highly divergent clusters were evident, supporting the recognition of two taxonomic units “C. dacica” and C. bigelowii “subsp. bigelowii”. Previously defined seed-sourcing regions for C. bigelowii s.lat. did not consider the known taxonomic complexity, and therefore interpreted the overall genetic structure as seed-sourcing regions, not taxa. We estimated genetic neighborhood sizes within each taxon to be 100–150 km and 300 km, respectively, indicating species-specific delimitations of local seed-sourcing regions. Frequent hybrids, local genetic distinctiveness, and suggested ecotypes add complexity to the discussed seed-sourcing regions. Our results show how genomic screening of diversity and structure in a species complex can alleviate the taxonomic impediment, inform practical questions, and legal requirements related to seed-sourcing, and together with traditional taxonomic work provide necessary information for a sound management of biodiversity. alpine ecological restoration, Carex bigelowii s.lat., ddRAD-seq, genetic structure, hybrids, seed-sourcing, subspecies, taxonomic impediment publishedVersion |
format |
Article in Journal/Newspaper |
author |
Westergaard, Kristine Bakke Kyrkjeeide, Magni Olsen Brandrud, Marie Kristine |
author_facet |
Westergaard, Kristine Bakke Kyrkjeeide, Magni Olsen Brandrud, Marie Kristine |
author_sort |
Westergaard, Kristine Bakke |
title |
Using genomics to guide seed-sourcing at the right taxonomical level for ecological restoration projects: The complex case of Carex bigelowii s.lat. in Norway |
title_short |
Using genomics to guide seed-sourcing at the right taxonomical level for ecological restoration projects: The complex case of Carex bigelowii s.lat. in Norway |
title_full |
Using genomics to guide seed-sourcing at the right taxonomical level for ecological restoration projects: The complex case of Carex bigelowii s.lat. in Norway |
title_fullStr |
Using genomics to guide seed-sourcing at the right taxonomical level for ecological restoration projects: The complex case of Carex bigelowii s.lat. in Norway |
title_full_unstemmed |
Using genomics to guide seed-sourcing at the right taxonomical level for ecological restoration projects: The complex case of Carex bigelowii s.lat. in Norway |
title_sort |
using genomics to guide seed-sourcing at the right taxonomical level for ecological restoration projects: the complex case of carex bigelowii s.lat. in norway |
publishDate |
2021 |
url |
https://hdl.handle.net/11250/2986529 https://doi.org/10.1002/ece3.8350 |
op_coverage |
Norway, Norge, Noreg |
geographic |
Norway |
geographic_facet |
Norway |
genre |
Carex bigelowii |
genre_facet |
Carex bigelowii |
op_source |
Ecology and Evolution |
op_relation |
Norges forskningsråd: 160022 urn:issn:2045-7758 https://hdl.handle.net/11250/2986529 https://doi.org/10.1002/ece3.8350 cristin:1957412 |
op_rights |
Navngivelse 4.0 Internasjonal http://creativecommons.org/licenses/by/4.0/deed.no © 2021 The Authors |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1002/ece3.8350 |
container_title |
Ecology and Evolution |
container_volume |
11 |
container_issue |
23 |
container_start_page |
17117 |
op_container_end_page |
17131 |
_version_ |
1766388418973007872 |