Genetic differentiation of Rubus chamaemorus populations in the Czech Republic and Norway after the last glacial period
The population structure of cloudberry (Rubus chamaemorus L.), collected from Krkonose Mountains (the Czech Republic), continental Norway and Spitsbergen, was examined using microsatellite analyses (SSR). Among 184 individuals, 162 different genotypes were identified. The overall unbiased gene diver...
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Online Access: | http://hdl.handle.net/11250/2498163 https://doi.org/10.1002/ece3.4101 |
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ftnibiocom:oai:brage.bibsys.no:11250/2498163 2023-05-15T18:07:45+02:00 Genetic differentiation of Rubus chamaemorus populations in the Czech Republic and Norway after the last glacial period Leišová‐Svobodová, Leona Phillips, Jade V. Martinussen, Inger Holubec, Vojtěch Czech Republic, Krkonose Mountains 2018-05-15T11:15:01Z http://hdl.handle.net/11250/2498163 https://doi.org/10.1002/ece3.4101 eng eng John Wiley & Sons Ltd. Czech‐Norwegian Research Programme. Grant Number: 7F14122 Ministerstvo Zemědělství. Grant Number: RO0414 Navngivelse 4.0 Internasjonal http://creativecommons.org/licenses/by/4.0/deed.no © 2018 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. CC-BY Ecology and Evolution cloudberry populations conservation genetic diversity microsatellites multivariate data analysis Journal article Peer reviewed 2018 ftnibiocom https://doi.org/10.1002/ece3.4101 2018-08-13T13:10:29Z The population structure of cloudberry (Rubus chamaemorus L.), collected from Krkonose Mountains (the Czech Republic), continental Norway and Spitsbergen, was examined using microsatellite analyses (SSR). Among 184 individuals, 162 different genotypes were identified. The overall unbiased gene diversity was high (urn:x-wiley:20457758:media:ece34101:ece34101-math-0001). A high level of genetic differentiation among populations (FST = 0.45; p < .01) indicated restricted gene flow between populations. Using a Bayesian approach, six clusters were found which represented the genetic structure of the studied cloudberry populations. The value of correlation index between genetic and geographical distances (r = .44) indicates that gene flow, even over a long distance, could exist. An exact test of population differentiation showed that Rubus chamaemorus populations from regions (Krkonose Mountains, continental Norway and Spitsbergen) are differentiated although some individuals within populations share common alleles even among regions. These results were confirmed by AMOVA, where the highest level of diversity was found within populations (70.8%). There was no difference between 87 pairs of populations (18.7%) mostly within cloudberry populations from continental Norway and from Spitsbergen. Based on obtained results, it is possible to conclude that Czech and Norwegian cloudberry populations are undergoing differentiation, which preserves unique allele compositions most likely from original populations during the last glaciation period. This knowledge will be important for the creation and continuation of in situ and ex situ conservation of cloudberry populations within these areas. publishedVersion Article in Journal/Newspaper Rubus chamaemorus Spitsbergen Norwegian Institute of Bioeconomy Research: NIBIO Brage Norway Ecology and Evolution 8 11 5701 5711 |
institution |
Open Polar |
collection |
Norwegian Institute of Bioeconomy Research: NIBIO Brage |
op_collection_id |
ftnibiocom |
language |
English |
topic |
cloudberry populations conservation genetic diversity microsatellites multivariate data analysis |
spellingShingle |
cloudberry populations conservation genetic diversity microsatellites multivariate data analysis Leišová‐Svobodová, Leona Phillips, Jade V. Martinussen, Inger Holubec, Vojtěch Genetic differentiation of Rubus chamaemorus populations in the Czech Republic and Norway after the last glacial period |
topic_facet |
cloudberry populations conservation genetic diversity microsatellites multivariate data analysis |
description |
The population structure of cloudberry (Rubus chamaemorus L.), collected from Krkonose Mountains (the Czech Republic), continental Norway and Spitsbergen, was examined using microsatellite analyses (SSR). Among 184 individuals, 162 different genotypes were identified. The overall unbiased gene diversity was high (urn:x-wiley:20457758:media:ece34101:ece34101-math-0001). A high level of genetic differentiation among populations (FST = 0.45; p < .01) indicated restricted gene flow between populations. Using a Bayesian approach, six clusters were found which represented the genetic structure of the studied cloudberry populations. The value of correlation index between genetic and geographical distances (r = .44) indicates that gene flow, even over a long distance, could exist. An exact test of population differentiation showed that Rubus chamaemorus populations from regions (Krkonose Mountains, continental Norway and Spitsbergen) are differentiated although some individuals within populations share common alleles even among regions. These results were confirmed by AMOVA, where the highest level of diversity was found within populations (70.8%). There was no difference between 87 pairs of populations (18.7%) mostly within cloudberry populations from continental Norway and from Spitsbergen. Based on obtained results, it is possible to conclude that Czech and Norwegian cloudberry populations are undergoing differentiation, which preserves unique allele compositions most likely from original populations during the last glaciation period. This knowledge will be important for the creation and continuation of in situ and ex situ conservation of cloudberry populations within these areas. publishedVersion |
format |
Article in Journal/Newspaper |
author |
Leišová‐Svobodová, Leona Phillips, Jade V. Martinussen, Inger Holubec, Vojtěch |
author_facet |
Leišová‐Svobodová, Leona Phillips, Jade V. Martinussen, Inger Holubec, Vojtěch |
author_sort |
Leišová‐Svobodová, Leona |
title |
Genetic differentiation of Rubus chamaemorus populations in the Czech Republic and Norway after the last glacial period |
title_short |
Genetic differentiation of Rubus chamaemorus populations in the Czech Republic and Norway after the last glacial period |
title_full |
Genetic differentiation of Rubus chamaemorus populations in the Czech Republic and Norway after the last glacial period |
title_fullStr |
Genetic differentiation of Rubus chamaemorus populations in the Czech Republic and Norway after the last glacial period |
title_full_unstemmed |
Genetic differentiation of Rubus chamaemorus populations in the Czech Republic and Norway after the last glacial period |
title_sort |
genetic differentiation of rubus chamaemorus populations in the czech republic and norway after the last glacial period |
publisher |
John Wiley & Sons Ltd. |
publishDate |
2018 |
url |
http://hdl.handle.net/11250/2498163 https://doi.org/10.1002/ece3.4101 |
op_coverage |
Czech Republic, Krkonose Mountains |
geographic |
Norway |
geographic_facet |
Norway |
genre |
Rubus chamaemorus Spitsbergen |
genre_facet |
Rubus chamaemorus Spitsbergen |
op_source |
Ecology and Evolution |
op_relation |
Czech‐Norwegian Research Programme. Grant Number: 7F14122 Ministerstvo Zemědělství. Grant Number: RO0414 |
op_rights |
Navngivelse 4.0 Internasjonal http://creativecommons.org/licenses/by/4.0/deed.no © 2018 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1002/ece3.4101 |
container_title |
Ecology and Evolution |
container_volume |
8 |
container_issue |
11 |
container_start_page |
5701 |
op_container_end_page |
5711 |
_version_ |
1766179966284726272 |