Emulsified nanoparticles containing inactivated influenza virus and CpG oligodeoxynucleotides critically influences the host immune responses in mice
Background: Antigen sparing and cross-protective immunity are regarded as crucial in pandemic influenza vaccine development. Both targets can be achieved by adjuvantation strategy to elicit a robust and broadened immune response. We assessed the immunogenicity of an inactivated H5N1 whole-virion vac...
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ftnhri:oai:ir.nhri.org.tw:3990099045/5023 2023-07-02T03:33:56+02:00 Emulsified nanoparticles containing inactivated influenza virus and CpG oligodeoxynucleotides critically influences the host immune responses in mice Huang, MH;Lin, SC;Hsiao, CH;Chao, HJ;Yang, HR;Liao, CC;Chuang, PW;Wu, HP;Huang, CY;Leng, CH;Liu, SJ;Chen, HW;Chou, AH;Hu, AYC;Chong, PL Vaccine Research and Development Center 2010-08 application/pdf 365961 bytes http://ir.nhri.org.tw/handle/3990099045/5023 http://ir.nhri.org.tw/bitstream/3990099045/5023/1/ISI000281075500013.pdf en-US en_US eng PUBLIC LIBRARY SCIENCE PLoS ONE. 2010 Aug;5(8):Article number e12279. 1932-6203 10.1371/journal.pone.0012279 http://ir.nhri.org.tw/handle/3990099045/5023 http://ir.nhri.org.tw/bitstream/3990099045/5023/1/ISI000281075500013.pdf Article 2010 ftnhri 2023-06-13T16:08:51Z Background: Antigen sparing and cross-protective immunity are regarded as crucial in pandemic influenza vaccine development. Both targets can be achieved by adjuvantation strategy to elicit a robust and broadened immune response. We assessed the immunogenicity of an inactivated H5N1 whole-virion vaccine (A/Vietnam/1194/2004 NIBRG-14, clade 1) formulated with emulsified nanoparticles and investigated whether it can induce cross-clade protecting immunity. Methodology/Principal Findings: After formulation with PELC, a proprietary water-in-oil-in-water nanoemulsion comprising of bioresorbable polymer/Span (R) 85/squalene, inactivated virus was intramuscularly administered to mice in either one-dose or two-dose schedule. We found that the antigen-specific serum antibody responses elicited after two doses of non-adjuvanted vaccine were lower than those observed after a single dose of adjuvanted vaccine, PELC and the conventional alum adjuvant as well. Moreover, 5 mu g HA of PELC-formulated inactivated virus were capable of inducing higher antibodies than those obtained from alum-adjuvanted vaccine. In single-dose study, we found that encapsulating inactivated virus into emulsified PELC nanoparticles could induce better antibody responses than those formulated with PELC-adsorbed vaccine. However, the potency was rather reduced when the inactivated virus and CpG (an immunostimulatory oligodeoxynucleotide containing unmethylated cytosine-guanosine motifs) were co-encapsulated within the emulsion. Finally, the mice who received PELC/CpG(adsorption)-vaccine could easily and quickly reach 100% of seroprotection against a homologous virus strain and effective cross-protection against a heterologous virus strain (A/Whooper swan/Mongolia/244/2005, clade 2.2). Conclusions/Significance: Encapsulating inactivated H5N1 influenza virus and CpG into emulsified nanoparticles critically influences the humoral responses against pandemic influenza. These results demonstrated that the use of PELC could be as antigen-sparing in ... Article in Journal/Newspaper Whooper Swan National Health Research Institutes (NHRI): Institutional Repository |
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National Health Research Institutes (NHRI): Institutional Repository |
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ftnhri |
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English |
description |
Background: Antigen sparing and cross-protective immunity are regarded as crucial in pandemic influenza vaccine development. Both targets can be achieved by adjuvantation strategy to elicit a robust and broadened immune response. We assessed the immunogenicity of an inactivated H5N1 whole-virion vaccine (A/Vietnam/1194/2004 NIBRG-14, clade 1) formulated with emulsified nanoparticles and investigated whether it can induce cross-clade protecting immunity. Methodology/Principal Findings: After formulation with PELC, a proprietary water-in-oil-in-water nanoemulsion comprising of bioresorbable polymer/Span (R) 85/squalene, inactivated virus was intramuscularly administered to mice in either one-dose or two-dose schedule. We found that the antigen-specific serum antibody responses elicited after two doses of non-adjuvanted vaccine were lower than those observed after a single dose of adjuvanted vaccine, PELC and the conventional alum adjuvant as well. Moreover, 5 mu g HA of PELC-formulated inactivated virus were capable of inducing higher antibodies than those obtained from alum-adjuvanted vaccine. In single-dose study, we found that encapsulating inactivated virus into emulsified PELC nanoparticles could induce better antibody responses than those formulated with PELC-adsorbed vaccine. However, the potency was rather reduced when the inactivated virus and CpG (an immunostimulatory oligodeoxynucleotide containing unmethylated cytosine-guanosine motifs) were co-encapsulated within the emulsion. Finally, the mice who received PELC/CpG(adsorption)-vaccine could easily and quickly reach 100% of seroprotection against a homologous virus strain and effective cross-protection against a heterologous virus strain (A/Whooper swan/Mongolia/244/2005, clade 2.2). Conclusions/Significance: Encapsulating inactivated H5N1 influenza virus and CpG into emulsified nanoparticles critically influences the humoral responses against pandemic influenza. These results demonstrated that the use of PELC could be as antigen-sparing in ... |
author2 |
Vaccine Research and Development Center |
format |
Article in Journal/Newspaper |
author |
Huang, MH;Lin, SC;Hsiao, CH;Chao, HJ;Yang, HR;Liao, CC;Chuang, PW;Wu, HP;Huang, CY;Leng, CH;Liu, SJ;Chen, HW;Chou, AH;Hu, AYC;Chong, PL |
spellingShingle |
Huang, MH;Lin, SC;Hsiao, CH;Chao, HJ;Yang, HR;Liao, CC;Chuang, PW;Wu, HP;Huang, CY;Leng, CH;Liu, SJ;Chen, HW;Chou, AH;Hu, AYC;Chong, PL Emulsified nanoparticles containing inactivated influenza virus and CpG oligodeoxynucleotides critically influences the host immune responses in mice |
author_facet |
Huang, MH;Lin, SC;Hsiao, CH;Chao, HJ;Yang, HR;Liao, CC;Chuang, PW;Wu, HP;Huang, CY;Leng, CH;Liu, SJ;Chen, HW;Chou, AH;Hu, AYC;Chong, PL |
author_sort |
Huang, MH;Lin, SC;Hsiao, CH;Chao, HJ;Yang, HR;Liao, CC;Chuang, PW;Wu, HP;Huang, CY;Leng, CH;Liu, SJ;Chen, HW;Chou, AH;Hu, AYC;Chong, PL |
title |
Emulsified nanoparticles containing inactivated influenza virus and CpG oligodeoxynucleotides critically influences the host immune responses in mice |
title_short |
Emulsified nanoparticles containing inactivated influenza virus and CpG oligodeoxynucleotides critically influences the host immune responses in mice |
title_full |
Emulsified nanoparticles containing inactivated influenza virus and CpG oligodeoxynucleotides critically influences the host immune responses in mice |
title_fullStr |
Emulsified nanoparticles containing inactivated influenza virus and CpG oligodeoxynucleotides critically influences the host immune responses in mice |
title_full_unstemmed |
Emulsified nanoparticles containing inactivated influenza virus and CpG oligodeoxynucleotides critically influences the host immune responses in mice |
title_sort |
emulsified nanoparticles containing inactivated influenza virus and cpg oligodeoxynucleotides critically influences the host immune responses in mice |
publisher |
PUBLIC LIBRARY SCIENCE |
publishDate |
2010 |
url |
http://ir.nhri.org.tw/handle/3990099045/5023 http://ir.nhri.org.tw/bitstream/3990099045/5023/1/ISI000281075500013.pdf |
genre |
Whooper Swan |
genre_facet |
Whooper Swan |
op_relation |
PLoS ONE. 2010 Aug;5(8):Article number e12279. 1932-6203 10.1371/journal.pone.0012279 http://ir.nhri.org.tw/handle/3990099045/5023 http://ir.nhri.org.tw/bitstream/3990099045/5023/1/ISI000281075500013.pdf |
_version_ |
1770274093941129216 |