The diversity, metabolomics profiling, and the pharmacological potential of actinomycetes isolated from the estremadura spur pockmarks (Portugal)
LA/P/0140/2020 PTDC/QUIQUI/119116/2010 Decree-Law 57/2016, of 29 August, changed by Law 57/2017, of 19 July. The Estremadura Spur pockmarks are a unique and unexplored ecosystem located in the North Atlantic, off the coast of Portugal. A total of 85 marine-derived actinomycetes were isolated and cul...
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Online Access: | http://hdl.handle.net/10362/132128 https://doi.org/10.3390/md20010021 |
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ftnewulisboa:oai:run.unl.pt:10362/132128 2023-05-15T17:34:45+02:00 The diversity, metabolomics profiling, and the pharmacological potential of actinomycetes isolated from the estremadura spur pockmarks (Portugal) Pinto-Almeida, António Bauermeister, Anelize Luppino, Luca Grilo, Inês R. Oliveira, Juliana Sousa, Joana R. Petras, Daniel Rodrigues, Clara F. Prieto-Davó, Alejandra Tasdemir, Deniz Sobral, Rita G. Gaudêncio, Susana P. UCIBIO - Applied Molecular Biosciences Unit DCV - Departamento de Ciências da Vida 2021-12-23 http://hdl.handle.net/10362/132128 https://doi.org/10.3390/md20010021 eng eng info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04378%2F2020/PT info:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FGEO-FIQ%2F5162%2F2014/PT info:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIA-MIC%2F31645%2F2017/PT info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50017%2F2020/PT info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50017%2F2020/PT info:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F148671%2F2019/PT 1660-3397 PURE: 36538807 PURE UUID: 3affbf27-8453-4e44-9ca8-56eceb388a93 Scopus: 85121613884 PubMed: 35049876 PubMedCentral: PMC8780274 WOS: 000747154100001 http://hdl.handle.net/10362/132128 https://doi.org/10.3390/md20010021 openAccess Actinobacteria Antimicrobial activity Blue biotechnology Estremadura Spur pockmarks Marine natural products Marine-derived actinomycetes Metabolomics Molecular networking Qemistree Drug Discovery SDG 14 - Life Below Water article 2021 ftnewulisboa https://doi.org/10.3390/md20010021 2023-03-22T01:07:21Z LA/P/0140/2020 PTDC/QUIQUI/119116/2010 Decree-Law 57/2016, of 29 August, changed by Law 57/2017, of 19 July. The Estremadura Spur pockmarks are a unique and unexplored ecosystem located in the North Atlantic, off the coast of Portugal. A total of 85 marine-derived actinomycetes were isolated and cultured from sediments collected from this ecosystem at a depth of 200 to 350 m. Nine genera, Streptomyces, Micromonospora, Saccharopolyspora, Actinomadura, Actinopolymorpha, Nocardiopsis, Saccharomonospora, Stackebrandtia, and Verrucosispora were identified by 16S rRNA gene sequencing analyses, from which the first two were the most predominant. Non-targeted LC-MS/MS, in combination with molecular networking, revealed high metabolite diversity, including several known metabolites, such as surugamide, antimycin, etamycin, physostigmine, desferrioxamine, ikarugamycin, piericidine, and rakicidin derivatives, as well as numerous unidentified metabolites. Taxonomy was the strongest parameter influencing the metabolite production, highlighting the different biosynthetic potentials of phylogenetically related actinomycetes; the majority of the chemical classes can be used as chemotaxonomic markers, as the metabolite distribution was mostly genera-specific. The EtOAc extracts of the actinomycete isolates demonstrated antimicrobial and antioxidant activity. Altogether, this study demonstrates that the Estremadura Spur is a source of actinomycetes with potential applications for biotechnology. It highlights the importance of investigating actinomycetes from unique ecosystems, such as pockmarks, as the metabolite production reflects their adaptation to this habitat. publishersversion published Article in Journal/Newspaper North Atlantic Repositório da Universidade Nova de Lisboa (UNL) Marine Drugs 20 1 21 |
institution |
Open Polar |
collection |
Repositório da Universidade Nova de Lisboa (UNL) |
op_collection_id |
ftnewulisboa |
language |
English |
topic |
Actinobacteria Antimicrobial activity Blue biotechnology Estremadura Spur pockmarks Marine natural products Marine-derived actinomycetes Metabolomics Molecular networking Qemistree Drug Discovery SDG 14 - Life Below Water |
spellingShingle |
Actinobacteria Antimicrobial activity Blue biotechnology Estremadura Spur pockmarks Marine natural products Marine-derived actinomycetes Metabolomics Molecular networking Qemistree Drug Discovery SDG 14 - Life Below Water Pinto-Almeida, António Bauermeister, Anelize Luppino, Luca Grilo, Inês R. Oliveira, Juliana Sousa, Joana R. Petras, Daniel Rodrigues, Clara F. Prieto-Davó, Alejandra Tasdemir, Deniz Sobral, Rita G. Gaudêncio, Susana P. The diversity, metabolomics profiling, and the pharmacological potential of actinomycetes isolated from the estremadura spur pockmarks (Portugal) |
topic_facet |
Actinobacteria Antimicrobial activity Blue biotechnology Estremadura Spur pockmarks Marine natural products Marine-derived actinomycetes Metabolomics Molecular networking Qemistree Drug Discovery SDG 14 - Life Below Water |
description |
LA/P/0140/2020 PTDC/QUIQUI/119116/2010 Decree-Law 57/2016, of 29 August, changed by Law 57/2017, of 19 July. The Estremadura Spur pockmarks are a unique and unexplored ecosystem located in the North Atlantic, off the coast of Portugal. A total of 85 marine-derived actinomycetes were isolated and cultured from sediments collected from this ecosystem at a depth of 200 to 350 m. Nine genera, Streptomyces, Micromonospora, Saccharopolyspora, Actinomadura, Actinopolymorpha, Nocardiopsis, Saccharomonospora, Stackebrandtia, and Verrucosispora were identified by 16S rRNA gene sequencing analyses, from which the first two were the most predominant. Non-targeted LC-MS/MS, in combination with molecular networking, revealed high metabolite diversity, including several known metabolites, such as surugamide, antimycin, etamycin, physostigmine, desferrioxamine, ikarugamycin, piericidine, and rakicidin derivatives, as well as numerous unidentified metabolites. Taxonomy was the strongest parameter influencing the metabolite production, highlighting the different biosynthetic potentials of phylogenetically related actinomycetes; the majority of the chemical classes can be used as chemotaxonomic markers, as the metabolite distribution was mostly genera-specific. The EtOAc extracts of the actinomycete isolates demonstrated antimicrobial and antioxidant activity. Altogether, this study demonstrates that the Estremadura Spur is a source of actinomycetes with potential applications for biotechnology. It highlights the importance of investigating actinomycetes from unique ecosystems, such as pockmarks, as the metabolite production reflects their adaptation to this habitat. publishersversion published |
author2 |
UCIBIO - Applied Molecular Biosciences Unit DCV - Departamento de Ciências da Vida |
format |
Article in Journal/Newspaper |
author |
Pinto-Almeida, António Bauermeister, Anelize Luppino, Luca Grilo, Inês R. Oliveira, Juliana Sousa, Joana R. Petras, Daniel Rodrigues, Clara F. Prieto-Davó, Alejandra Tasdemir, Deniz Sobral, Rita G. Gaudêncio, Susana P. |
author_facet |
Pinto-Almeida, António Bauermeister, Anelize Luppino, Luca Grilo, Inês R. Oliveira, Juliana Sousa, Joana R. Petras, Daniel Rodrigues, Clara F. Prieto-Davó, Alejandra Tasdemir, Deniz Sobral, Rita G. Gaudêncio, Susana P. |
author_sort |
Pinto-Almeida, António |
title |
The diversity, metabolomics profiling, and the pharmacological potential of actinomycetes isolated from the estremadura spur pockmarks (Portugal) |
title_short |
The diversity, metabolomics profiling, and the pharmacological potential of actinomycetes isolated from the estremadura spur pockmarks (Portugal) |
title_full |
The diversity, metabolomics profiling, and the pharmacological potential of actinomycetes isolated from the estremadura spur pockmarks (Portugal) |
title_fullStr |
The diversity, metabolomics profiling, and the pharmacological potential of actinomycetes isolated from the estremadura spur pockmarks (Portugal) |
title_full_unstemmed |
The diversity, metabolomics profiling, and the pharmacological potential of actinomycetes isolated from the estremadura spur pockmarks (Portugal) |
title_sort |
diversity, metabolomics profiling, and the pharmacological potential of actinomycetes isolated from the estremadura spur pockmarks (portugal) |
publishDate |
2021 |
url |
http://hdl.handle.net/10362/132128 https://doi.org/10.3390/md20010021 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_relation |
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04378%2F2020/PT info:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FGEO-FIQ%2F5162%2F2014/PT info:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIA-MIC%2F31645%2F2017/PT info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50017%2F2020/PT info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50017%2F2020/PT info:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F148671%2F2019/PT 1660-3397 PURE: 36538807 PURE UUID: 3affbf27-8453-4e44-9ca8-56eceb388a93 Scopus: 85121613884 PubMed: 35049876 PubMedCentral: PMC8780274 WOS: 000747154100001 http://hdl.handle.net/10362/132128 https://doi.org/10.3390/md20010021 |
op_rights |
openAccess |
op_doi |
https://doi.org/10.3390/md20010021 |
container_title |
Marine Drugs |
container_volume |
20 |
container_issue |
1 |
container_start_page |
21 |
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1766133680654254080 |