Biocontrol mechanisms of the Antarctic yeast Debaryomyces hansenii UFT8244 against post-harvest phytopathogenic fungi of strawberries
The use of yeasts has been explored as an efficient alternative to fungicide application in the treatment and prevention of post-harvest fruit deterioration. Here, we evaluated the biocontrol abilities of the Antarctic yeast strain Debaryomyces hansenii UFT8244 against the post-harvest phytopathogen...
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Academia Brasileira de Ciências
2024
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Online Access: | http://nora.nerc.ac.uk/id/eprint/536402/ https://nora.nerc.ac.uk/id/eprint/536402/1/Ferreira_Biocontrol_mechanisms_2024.pdf https://www.scielo.br/j/aabc/a/GD7kbSYJd6KRzLG4CZV3Gbs/?lang=en https://doi.org/10.1590/0001-3765202420240255 |
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ftnerc:oai:nora.nerc.ac.uk:536402 2024-09-15T17:41:45+00:00 Biocontrol mechanisms of the Antarctic yeast Debaryomyces hansenii UFT8244 against post-harvest phytopathogenic fungi of strawberries Ferreira, Eskálath Morganna S. Garmendia, Gabriela Gonçalves, Vívian N. da Silva, Juliana F.M. Convey, Peter Rosa, Luiz Henrique Vero, Silvana Pimenta, Raphael S. 2024-08 text http://nora.nerc.ac.uk/id/eprint/536402/ https://nora.nerc.ac.uk/id/eprint/536402/1/Ferreira_Biocontrol_mechanisms_2024.pdf https://www.scielo.br/j/aabc/a/GD7kbSYJd6KRzLG4CZV3Gbs/?lang=en https://doi.org/10.1590/0001-3765202420240255 en eng Academia Brasileira de Ciências https://nora.nerc.ac.uk/id/eprint/536402/1/Ferreira_Biocontrol_mechanisms_2024.pdf Ferreira, Eskálath Morganna S.; Garmendia, Gabriela; Gonçalves, Vívian N.; da Silva, Juliana F.M.; Convey, Peter orcid:0000-0001-8497-9903 Rosa, Luiz Henrique; Vero, Silvana; Pimenta, Raphael S. 2024 Biocontrol mechanisms of the Antarctic yeast Debaryomyces hansenii UFT8244 against post-harvest phytopathogenic fungi of strawberries. Anais da Academia Brasileira de Ciências, 96 (Suppl. 2), e20240255. 11, pp. https://doi.org/10.1590/0001-3765202420240255 <https://doi.org/10.1590/0001-3765202420240255> cc_by_4 Publication - Article PeerReviewed 2024 ftnerc https://doi.org/10.1590/0001-3765202420240255 2024-09-03T23:47:14Z The use of yeasts has been explored as an efficient alternative to fungicide application in the treatment and prevention of post-harvest fruit deterioration. Here, we evaluated the biocontrol abilities of the Antarctic yeast strain Debaryomyces hansenii UFT8244 against the post-harvest phytopathogenic fungi Botrytis cinerea and Rhizopus stolonifer for the protection and preservation of strawberry fruit. The strongest inhibition of germination of B. cinerea (57%) was observed at 0 °C, followed by 40% at 25 °C. In addition, germ tubes and hyphae of B. cinerea were strongly surrounded and colonized by D. hansenii. Production of the enzymes β-1,3-glucanase, chitinase and protease by D. hansenii was detected in the presence of phytopathogenic fungus cell walls. The activity of β-1,3-glucanase was highest on day 12 of incubation and remained high until day 15. Chitinase and protease activities reached their highest levels on the day 15 of incubation. D. hansenii additionally demonstrated the ability to resist oxidative stress. Our data demonstrated that the main biocontrol mechanisms displayed by D. hansenii were the control of phytopathogenic fungal spore germination, production of antifungal enzymes and resistance to oxidative stress. We conclude that isolate D. hansenii UFT8422 should be further investigated for use at commercial scales at low temperatures. Article in Journal/Newspaper Antarc* Antarctic Natural Environment Research Council: NERC Open Research Archive Anais da Academia Brasileira de Ciências 96 suppl 2 |
institution |
Open Polar |
collection |
Natural Environment Research Council: NERC Open Research Archive |
op_collection_id |
ftnerc |
language |
English |
description |
The use of yeasts has been explored as an efficient alternative to fungicide application in the treatment and prevention of post-harvest fruit deterioration. Here, we evaluated the biocontrol abilities of the Antarctic yeast strain Debaryomyces hansenii UFT8244 against the post-harvest phytopathogenic fungi Botrytis cinerea and Rhizopus stolonifer for the protection and preservation of strawberry fruit. The strongest inhibition of germination of B. cinerea (57%) was observed at 0 °C, followed by 40% at 25 °C. In addition, germ tubes and hyphae of B. cinerea were strongly surrounded and colonized by D. hansenii. Production of the enzymes β-1,3-glucanase, chitinase and protease by D. hansenii was detected in the presence of phytopathogenic fungus cell walls. The activity of β-1,3-glucanase was highest on day 12 of incubation and remained high until day 15. Chitinase and protease activities reached their highest levels on the day 15 of incubation. D. hansenii additionally demonstrated the ability to resist oxidative stress. Our data demonstrated that the main biocontrol mechanisms displayed by D. hansenii were the control of phytopathogenic fungal spore germination, production of antifungal enzymes and resistance to oxidative stress. We conclude that isolate D. hansenii UFT8422 should be further investigated for use at commercial scales at low temperatures. |
format |
Article in Journal/Newspaper |
author |
Ferreira, Eskálath Morganna S. Garmendia, Gabriela Gonçalves, Vívian N. da Silva, Juliana F.M. Convey, Peter Rosa, Luiz Henrique Vero, Silvana Pimenta, Raphael S. |
spellingShingle |
Ferreira, Eskálath Morganna S. Garmendia, Gabriela Gonçalves, Vívian N. da Silva, Juliana F.M. Convey, Peter Rosa, Luiz Henrique Vero, Silvana Pimenta, Raphael S. Biocontrol mechanisms of the Antarctic yeast Debaryomyces hansenii UFT8244 against post-harvest phytopathogenic fungi of strawberries |
author_facet |
Ferreira, Eskálath Morganna S. Garmendia, Gabriela Gonçalves, Vívian N. da Silva, Juliana F.M. Convey, Peter Rosa, Luiz Henrique Vero, Silvana Pimenta, Raphael S. |
author_sort |
Ferreira, Eskálath Morganna S. |
title |
Biocontrol mechanisms of the Antarctic yeast Debaryomyces hansenii UFT8244 against post-harvest phytopathogenic fungi of strawberries |
title_short |
Biocontrol mechanisms of the Antarctic yeast Debaryomyces hansenii UFT8244 against post-harvest phytopathogenic fungi of strawberries |
title_full |
Biocontrol mechanisms of the Antarctic yeast Debaryomyces hansenii UFT8244 against post-harvest phytopathogenic fungi of strawberries |
title_fullStr |
Biocontrol mechanisms of the Antarctic yeast Debaryomyces hansenii UFT8244 against post-harvest phytopathogenic fungi of strawberries |
title_full_unstemmed |
Biocontrol mechanisms of the Antarctic yeast Debaryomyces hansenii UFT8244 against post-harvest phytopathogenic fungi of strawberries |
title_sort |
biocontrol mechanisms of the antarctic yeast debaryomyces hansenii uft8244 against post-harvest phytopathogenic fungi of strawberries |
publisher |
Academia Brasileira de Ciências |
publishDate |
2024 |
url |
http://nora.nerc.ac.uk/id/eprint/536402/ https://nora.nerc.ac.uk/id/eprint/536402/1/Ferreira_Biocontrol_mechanisms_2024.pdf https://www.scielo.br/j/aabc/a/GD7kbSYJd6KRzLG4CZV3Gbs/?lang=en https://doi.org/10.1590/0001-3765202420240255 |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
https://nora.nerc.ac.uk/id/eprint/536402/1/Ferreira_Biocontrol_mechanisms_2024.pdf Ferreira, Eskálath Morganna S.; Garmendia, Gabriela; Gonçalves, Vívian N.; da Silva, Juliana F.M.; Convey, Peter orcid:0000-0001-8497-9903 Rosa, Luiz Henrique; Vero, Silvana; Pimenta, Raphael S. 2024 Biocontrol mechanisms of the Antarctic yeast Debaryomyces hansenii UFT8244 against post-harvest phytopathogenic fungi of strawberries. Anais da Academia Brasileira de Ciências, 96 (Suppl. 2), e20240255. 11, pp. https://doi.org/10.1590/0001-3765202420240255 <https://doi.org/10.1590/0001-3765202420240255> |
op_rights |
cc_by_4 |
op_doi |
https://doi.org/10.1590/0001-3765202420240255 |
container_title |
Anais da Academia Brasileira de Ciências |
container_volume |
96 |
container_issue |
suppl 2 |
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1810488004514414592 |