Fungal and fungal-like diversity in marine sediments from the maritime Antarctic assessed using DNA metabarcoding
We assessed the fungal and fungal-like sequence diversity present in marine sediments obtained in the vicinity of the South Shetland Islands (Southern Ocean) using DNA metabarcoding through high-throughput sequencing (HTS). A total of 193,436 DNA reads were detected in sediment obtained from three l...
Published in: | Scientific Reports |
---|---|
Main Authors: | , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Springer Nature
2022
|
Subjects: | |
Online Access: | http://nora.nerc.ac.uk/id/eprint/533644/ https://nora.nerc.ac.uk/id/eprint/533644/1/s41598-022-25310-2.pdf https://www.nature.com/articles/s41598-022-25310-2 |
id |
ftnerc:oai:nora.nerc.ac.uk:533644 |
---|---|
record_format |
openpolar |
spelling |
ftnerc:oai:nora.nerc.ac.uk:533644 2023-05-15T14:02:21+02:00 Fungal and fungal-like diversity in marine sediments from the maritime Antarctic assessed using DNA metabarcoding da Silva, Mayanne Karla de Souza, Láuren Machado Drumond Vieira, Rosemary Neto, Arthur Ayres Lopes, Fabyano A.C. de Oliveira, Fábio S. Convey, Peter Carvalho-Silva, Micheline Duarte, Alysson Wagner Fernandes Câmara, Paulo E.A.S. Rosa, Luiz Henrique 2022-12-06 text http://nora.nerc.ac.uk/id/eprint/533644/ https://nora.nerc.ac.uk/id/eprint/533644/1/s41598-022-25310-2.pdf https://www.nature.com/articles/s41598-022-25310-2 en eng Springer Nature https://nora.nerc.ac.uk/id/eprint/533644/1/s41598-022-25310-2.pdf da Silva, Mayanne Karla; de Souza, Láuren Machado Drumond; Vieira, Rosemary; Neto, Arthur Ayres; Lopes, Fabyano A.C.; de Oliveira, Fábio S.; Convey, Peter orcid:0000-0001-8497-9903 Carvalho-Silva, Micheline; Duarte, Alysson Wagner Fernandes; Câmara, Paulo E.A.S.; Rosa, Luiz Henrique. 2022 Fungal and fungal-like diversity in marine sediments from the maritime Antarctic assessed using DNA metabarcoding. Scientific Reports, 12, 21044. 10, pp. https://doi.org/10.1038/s41598-022-25310-2 <https://doi.org/10.1038/s41598-022-25310-2> cc_by_4 CC-BY Publication - Article PeerReviewed 2022 ftnerc https://doi.org/10.1038/s41598-022-25310-2 2023-02-04T19:53:48Z We assessed the fungal and fungal-like sequence diversity present in marine sediments obtained in the vicinity of the South Shetland Islands (Southern Ocean) using DNA metabarcoding through high-throughput sequencing (HTS). A total of 193,436 DNA reads were detected in sediment obtained from three locations: Walker Bay (Livingston Island) at 52 m depth (48,112 reads), Whalers Bay (Deception Island) at 151 m (104,704) and English Strait at 404 m (40,620). The DNA sequence reads were assigned to 133 distinct fungal amplicon sequence variants (ASVs) representing the phyla Ascomycota, Basidiomycota, Mortierellomycota, Chytridiomycota, Glomeromycota, Monoblepharomycota, Mucoromycota and Rozellomycota and the fungal-like Straminopila. Thelebolus balaustiformis, Pseudogymnoascus sp., Fungi sp. 1, Ciliophora sp., Agaricomycetes sp. and Chaetoceros sp. were the dominant assigned taxa. Thirty-eight fungal ASVs could only be assigned to higher taxonomic levels, and may represent taxa not currently included in the available databases or represent new taxa and/or new records for Antarctica. The total fungal community displayed high indices of diversity, richness and moderate to low dominance. However, diversity and taxa distribution varied across the three sampling sites. In Walker Bay, unidentified fungi were dominant in the sequence assemblage. Whalers Bay sediment was dominated by Antarctic endemic and cold-adapted taxa. Sediment from English Strait was dominated by Ciliophora sp. and Chaetoceros sp. These fungal assemblages were dominated by saprotrophic, plant and animal pathogenic and symbiotic taxa. The detection of an apparently rich and diverse fungal community in these marine sediments reinforces the need for further studies to characterize their richness, functional ecology and potential biotechnological applications. Article in Journal/Newspaper Antarc* Antarctic Antarctica Deception Island Livingston Island South Shetland Islands Southern Ocean Walker Bay Natural Environment Research Council: NERC Open Research Archive Antarctic Southern Ocean South Shetland Islands Deception Island ENVELOPE(-60.633,-60.633,-62.950,-62.950) Livingston Island ENVELOPE(-60.500,-60.500,-62.600,-62.600) Whalers Bay ENVELOPE(69.917,69.917,-49.100,-49.100) Walker Bay ENVELOPE(-60.700,-60.700,-62.633,-62.633) English Strait ENVELOPE(-59.633,-59.633,-62.450,-62.450) Scientific Reports 12 1 |
institution |
Open Polar |
collection |
Natural Environment Research Council: NERC Open Research Archive |
op_collection_id |
ftnerc |
language |
English |
description |
We assessed the fungal and fungal-like sequence diversity present in marine sediments obtained in the vicinity of the South Shetland Islands (Southern Ocean) using DNA metabarcoding through high-throughput sequencing (HTS). A total of 193,436 DNA reads were detected in sediment obtained from three locations: Walker Bay (Livingston Island) at 52 m depth (48,112 reads), Whalers Bay (Deception Island) at 151 m (104,704) and English Strait at 404 m (40,620). The DNA sequence reads were assigned to 133 distinct fungal amplicon sequence variants (ASVs) representing the phyla Ascomycota, Basidiomycota, Mortierellomycota, Chytridiomycota, Glomeromycota, Monoblepharomycota, Mucoromycota and Rozellomycota and the fungal-like Straminopila. Thelebolus balaustiformis, Pseudogymnoascus sp., Fungi sp. 1, Ciliophora sp., Agaricomycetes sp. and Chaetoceros sp. were the dominant assigned taxa. Thirty-eight fungal ASVs could only be assigned to higher taxonomic levels, and may represent taxa not currently included in the available databases or represent new taxa and/or new records for Antarctica. The total fungal community displayed high indices of diversity, richness and moderate to low dominance. However, diversity and taxa distribution varied across the three sampling sites. In Walker Bay, unidentified fungi were dominant in the sequence assemblage. Whalers Bay sediment was dominated by Antarctic endemic and cold-adapted taxa. Sediment from English Strait was dominated by Ciliophora sp. and Chaetoceros sp. These fungal assemblages were dominated by saprotrophic, plant and animal pathogenic and symbiotic taxa. The detection of an apparently rich and diverse fungal community in these marine sediments reinforces the need for further studies to characterize their richness, functional ecology and potential biotechnological applications. |
format |
Article in Journal/Newspaper |
author |
da Silva, Mayanne Karla de Souza, Láuren Machado Drumond Vieira, Rosemary Neto, Arthur Ayres Lopes, Fabyano A.C. de Oliveira, Fábio S. Convey, Peter Carvalho-Silva, Micheline Duarte, Alysson Wagner Fernandes Câmara, Paulo E.A.S. Rosa, Luiz Henrique |
spellingShingle |
da Silva, Mayanne Karla de Souza, Láuren Machado Drumond Vieira, Rosemary Neto, Arthur Ayres Lopes, Fabyano A.C. de Oliveira, Fábio S. Convey, Peter Carvalho-Silva, Micheline Duarte, Alysson Wagner Fernandes Câmara, Paulo E.A.S. Rosa, Luiz Henrique Fungal and fungal-like diversity in marine sediments from the maritime Antarctic assessed using DNA metabarcoding |
author_facet |
da Silva, Mayanne Karla de Souza, Láuren Machado Drumond Vieira, Rosemary Neto, Arthur Ayres Lopes, Fabyano A.C. de Oliveira, Fábio S. Convey, Peter Carvalho-Silva, Micheline Duarte, Alysson Wagner Fernandes Câmara, Paulo E.A.S. Rosa, Luiz Henrique |
author_sort |
da Silva, Mayanne Karla |
title |
Fungal and fungal-like diversity in marine sediments from the maritime Antarctic assessed using DNA metabarcoding |
title_short |
Fungal and fungal-like diversity in marine sediments from the maritime Antarctic assessed using DNA metabarcoding |
title_full |
Fungal and fungal-like diversity in marine sediments from the maritime Antarctic assessed using DNA metabarcoding |
title_fullStr |
Fungal and fungal-like diversity in marine sediments from the maritime Antarctic assessed using DNA metabarcoding |
title_full_unstemmed |
Fungal and fungal-like diversity in marine sediments from the maritime Antarctic assessed using DNA metabarcoding |
title_sort |
fungal and fungal-like diversity in marine sediments from the maritime antarctic assessed using dna metabarcoding |
publisher |
Springer Nature |
publishDate |
2022 |
url |
http://nora.nerc.ac.uk/id/eprint/533644/ https://nora.nerc.ac.uk/id/eprint/533644/1/s41598-022-25310-2.pdf https://www.nature.com/articles/s41598-022-25310-2 |
long_lat |
ENVELOPE(-60.633,-60.633,-62.950,-62.950) ENVELOPE(-60.500,-60.500,-62.600,-62.600) ENVELOPE(69.917,69.917,-49.100,-49.100) ENVELOPE(-60.700,-60.700,-62.633,-62.633) ENVELOPE(-59.633,-59.633,-62.450,-62.450) |
geographic |
Antarctic Southern Ocean South Shetland Islands Deception Island Livingston Island Whalers Bay Walker Bay English Strait |
geographic_facet |
Antarctic Southern Ocean South Shetland Islands Deception Island Livingston Island Whalers Bay Walker Bay English Strait |
genre |
Antarc* Antarctic Antarctica Deception Island Livingston Island South Shetland Islands Southern Ocean Walker Bay |
genre_facet |
Antarc* Antarctic Antarctica Deception Island Livingston Island South Shetland Islands Southern Ocean Walker Bay |
op_relation |
https://nora.nerc.ac.uk/id/eprint/533644/1/s41598-022-25310-2.pdf da Silva, Mayanne Karla; de Souza, Láuren Machado Drumond; Vieira, Rosemary; Neto, Arthur Ayres; Lopes, Fabyano A.C.; de Oliveira, Fábio S.; Convey, Peter orcid:0000-0001-8497-9903 Carvalho-Silva, Micheline; Duarte, Alysson Wagner Fernandes; Câmara, Paulo E.A.S.; Rosa, Luiz Henrique. 2022 Fungal and fungal-like diversity in marine sediments from the maritime Antarctic assessed using DNA metabarcoding. Scientific Reports, 12, 21044. 10, pp. https://doi.org/10.1038/s41598-022-25310-2 <https://doi.org/10.1038/s41598-022-25310-2> |
op_rights |
cc_by_4 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1038/s41598-022-25310-2 |
container_title |
Scientific Reports |
container_volume |
12 |
container_issue |
1 |
_version_ |
1766272566432890880 |