Microbiomic analysis of bacteria associated with rock tripe lichens in Continental and Maritime Antarctic regions
Increased research attention is being given to bacterial diversity associated with lichens. Rock tripe lichens (Umbilicariaceae) were collected from two distinct Antarctic biological regions, the continental region near the Japanese Antarctic station (Syowa Station) and the maritime Antarctic South...
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ftnerc:oai:nora.nerc.ac.uk:532714 2023-05-15T13:41:46+02:00 Microbiomic analysis of bacteria associated with rock tripe lichens in Continental and Maritime Antarctic regions He, Zichen Naganuma, Takeshi Nakai, Ryosuke Imura, Satoshi Tsujimoto, Megumu Convey, Peter 2022-08-03 text http://nora.nerc.ac.uk/id/eprint/532714/ https://nora.nerc.ac.uk/id/eprint/532714/1/jof-08-00817.pdf https://www.mdpi.com/2309-608X/8/8/817 en eng MDPI https://nora.nerc.ac.uk/id/eprint/532714/1/jof-08-00817.pdf He, Zichen; Naganuma, Takeshi; Nakai, Ryosuke; Imura, Satoshi; Tsujimoto, Megumu; Convey, Peter orcid:0000-0001-8497-9903 . 2022 Microbiomic analysis of bacteria associated with rock tripe lichens in Continental and Maritime Antarctic regions [in special issue: Ecology and Evolution of Lichens and Associated Microorganisms] Journal of Fungi, 8 (8), 817. 17, pp. https://doi.org/10.3390/jof8080817 <https://doi.org/10.3390/jof8080817> cc_by_4 CC-BY Publication - Article PeerReviewed 2022 ftnerc https://doi.org/10.3390/jof8080817 2023-02-04T19:53:18Z Increased research attention is being given to bacterial diversity associated with lichens. Rock tripe lichens (Umbilicariaceae) were collected from two distinct Antarctic biological regions, the continental region near the Japanese Antarctic station (Syowa Station) and the maritime Antarctic South Orkney Islands (Signy Island), in order to compare their bacterial floras and potential metabolism. Bulk DNA extracted from the lichen samples was used to amplify the 18S rRNA gene and the V3-V4 region of the 16S rRNA gene, whose amplicons were Sanger- and MiSeq-sequenced, respectively. The fungal and algal partners represented members of the ascomycete genus Umbilicaria and the green algal genus Trebouxia, based on 18S rRNA gene sequences. The V3-V4 sequences were grouped into operational taxonomic units (OTUs), which were assigned to eight bacterial phyla, Acidobacteriota, Actinomyceota, Armatimonadota, Bacteroidota, Cyanobacteria, Deinococcota, Pseudomonadota and the candidate phylum Saccharibacteria (also known as TM7), commonly present in all samples. The OTU floras of the two biological regions were clearly distinct, with regional biomarker genera, such as Mucilaginibacter and Gluconacetobacter, respectively. The OTU-based metabolism analysis predicted higher membrane transport activities in the maritime Antarctic OTUs, probably influenced by the sampling area’s warmer maritime climatic setting. Article in Journal/Newspaper Antarc* Antarctic Signy Island South Orkney Islands Natural Environment Research Council: NERC Open Research Archive Antarctic Syowa Station South Orkney Islands ENVELOPE(-45.500,-45.500,-60.583,-60.583) Signy Island ENVELOPE(-45.595,-45.595,-60.708,-60.708) Journal of Fungi 8 8 817 |
institution |
Open Polar |
collection |
Natural Environment Research Council: NERC Open Research Archive |
op_collection_id |
ftnerc |
language |
English |
description |
Increased research attention is being given to bacterial diversity associated with lichens. Rock tripe lichens (Umbilicariaceae) were collected from two distinct Antarctic biological regions, the continental region near the Japanese Antarctic station (Syowa Station) and the maritime Antarctic South Orkney Islands (Signy Island), in order to compare their bacterial floras and potential metabolism. Bulk DNA extracted from the lichen samples was used to amplify the 18S rRNA gene and the V3-V4 region of the 16S rRNA gene, whose amplicons were Sanger- and MiSeq-sequenced, respectively. The fungal and algal partners represented members of the ascomycete genus Umbilicaria and the green algal genus Trebouxia, based on 18S rRNA gene sequences. The V3-V4 sequences were grouped into operational taxonomic units (OTUs), which were assigned to eight bacterial phyla, Acidobacteriota, Actinomyceota, Armatimonadota, Bacteroidota, Cyanobacteria, Deinococcota, Pseudomonadota and the candidate phylum Saccharibacteria (also known as TM7), commonly present in all samples. The OTU floras of the two biological regions were clearly distinct, with regional biomarker genera, such as Mucilaginibacter and Gluconacetobacter, respectively. The OTU-based metabolism analysis predicted higher membrane transport activities in the maritime Antarctic OTUs, probably influenced by the sampling area’s warmer maritime climatic setting. |
format |
Article in Journal/Newspaper |
author |
He, Zichen Naganuma, Takeshi Nakai, Ryosuke Imura, Satoshi Tsujimoto, Megumu Convey, Peter |
spellingShingle |
He, Zichen Naganuma, Takeshi Nakai, Ryosuke Imura, Satoshi Tsujimoto, Megumu Convey, Peter Microbiomic analysis of bacteria associated with rock tripe lichens in Continental and Maritime Antarctic regions |
author_facet |
He, Zichen Naganuma, Takeshi Nakai, Ryosuke Imura, Satoshi Tsujimoto, Megumu Convey, Peter |
author_sort |
He, Zichen |
title |
Microbiomic analysis of bacteria associated with rock tripe lichens in Continental and Maritime Antarctic regions |
title_short |
Microbiomic analysis of bacteria associated with rock tripe lichens in Continental and Maritime Antarctic regions |
title_full |
Microbiomic analysis of bacteria associated with rock tripe lichens in Continental and Maritime Antarctic regions |
title_fullStr |
Microbiomic analysis of bacteria associated with rock tripe lichens in Continental and Maritime Antarctic regions |
title_full_unstemmed |
Microbiomic analysis of bacteria associated with rock tripe lichens in Continental and Maritime Antarctic regions |
title_sort |
microbiomic analysis of bacteria associated with rock tripe lichens in continental and maritime antarctic regions |
publisher |
MDPI |
publishDate |
2022 |
url |
http://nora.nerc.ac.uk/id/eprint/532714/ https://nora.nerc.ac.uk/id/eprint/532714/1/jof-08-00817.pdf https://www.mdpi.com/2309-608X/8/8/817 |
long_lat |
ENVELOPE(-45.500,-45.500,-60.583,-60.583) ENVELOPE(-45.595,-45.595,-60.708,-60.708) |
geographic |
Antarctic Syowa Station South Orkney Islands Signy Island |
geographic_facet |
Antarctic Syowa Station South Orkney Islands Signy Island |
genre |
Antarc* Antarctic Signy Island South Orkney Islands |
genre_facet |
Antarc* Antarctic Signy Island South Orkney Islands |
op_relation |
https://nora.nerc.ac.uk/id/eprint/532714/1/jof-08-00817.pdf He, Zichen; Naganuma, Takeshi; Nakai, Ryosuke; Imura, Satoshi; Tsujimoto, Megumu; Convey, Peter orcid:0000-0001-8497-9903 . 2022 Microbiomic analysis of bacteria associated with rock tripe lichens in Continental and Maritime Antarctic regions [in special issue: Ecology and Evolution of Lichens and Associated Microorganisms] Journal of Fungi, 8 (8), 817. 17, pp. https://doi.org/10.3390/jof8080817 <https://doi.org/10.3390/jof8080817> |
op_rights |
cc_by_4 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3390/jof8080817 |
container_title |
Journal of Fungi |
container_volume |
8 |
container_issue |
8 |
container_start_page |
817 |
_version_ |
1766157896080424960 |