Fungal diversity in a sediment core from climate change impacted Boeckella Lake, Hope Bay, north-eastern Antarctic Peninsula assessed using metabarcoding

We studied the fungal DNA present in a lake sediment core obtained from Trinity Peninsula, Hope Bay, north-eastern Antarctic Peninsula, using metabarcoding through high-throughput sequencing (HTS). Sequences obtained were assigned to 146 amplicon sequence variants (ASVs) primarily representing unkno...

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Bibliographic Details
Published in:Extremophiles
Main Authors: Rosa, Luiz Henrique, Ogaki, Mayara Baptistucci, Lirio, Juan Manuel, Vieira, Rosemary, Coria, Silvia H., Pinto, Otávio Henrique Bezerra, Carvalho-Silva, Micheline, Convey, Peter, Rosa, Carlos Augusto, Câmara, Paulo Eduardo Aguiar Saraiva
Format: Article in Journal/Newspaper
Language:unknown
Published: Springer 2022
Subjects:
Antarctic
Antarctic Peninsula
Boeckella
Hope Bay
Trinity Peninsula
Lake May
Lake Hope
Boeckella Lake
Antarc*
Online Access:http://nora.nerc.ac.uk/id/eprint/531475/
https://rdcu.be/cM9w3
Description
Summary:We studied the fungal DNA present in a lake sediment core obtained from Trinity Peninsula, Hope Bay, north-eastern Antarctic Peninsula, using metabarcoding through high-throughput sequencing (HTS). Sequences obtained were assigned to 146 amplicon sequence variants (ASVs) primarily representing unknown fungi, followed by the phyla Ascomycota, Rozellomycota, Basidiomycota, Chytridiomycota and Mortierellomycota. The most abundant taxa were assigned to Fungal sp., Pseudeurotium hygrophilum, Rozellomycota sp. 1, Pseudeurotiaceae sp. 1 and Chytridiomycota sp. 1. The majority of the DNA reads, representing 40 ASVs, could only be assigned at higher taxonomic levels and may represent taxa not currently included in the sequence databases consulted and/or be previously undescribed fungi. Different sections of the core were characterized by high sequence diversity, richness and moderate ecological dominance indices. The assigned diversity was dominated by cosmopolitan cold-adapted fungi, including known saprotrophic, plant and animal pathogenic and symbiotic taxa. Despite the overall dominance of Ascomycota and Basidiomycota and psychrophilic Mortierellomycota, members of the cryptic phyla Rozellomycota and Chytridiomycota were also detected in abundance. As Boeckella Lake may cease to exist in approaching decades due the effects of local climatic changes, it also an important location for the study of the impacts of these changes on Antarctic microbial diversity.

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