Statistical optimisation and kinetic studies of molybdenum reduction using a psychrotolerant marine bacteria isolated from Antarctica
The extensive industrial use of the heavy metal molybdenum (Mo) has led to an emerging global pollution with its traces that can even be found in Antarctica. In response, a reduction process that transforms hexamolybdate (Mo6+) to a less toxic compound, Mo-blue, using microorganisms provides a susta...
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ftnerc:oai:nora.nerc.ac.uk:529980 2023-05-15T13:41:45+02:00 Statistical optimisation and kinetic studies of molybdenum reduction using a psychrotolerant marine bacteria isolated from Antarctica Darham, Syazani Zahri, Khadijah Nabilah Mohd Zulkharnain, Azham Sabri, Suriana Gomez-Fuentes, Claudio Convey, Peter Khalil, Khalilah Abdul Ahmad, Siti Aqlima 2021-06-11 text http://nora.nerc.ac.uk/id/eprint/529980/ https://nora.nerc.ac.uk/id/eprint/529980/1/jmse-09-00648.pdf https://www.mdpi.com/2077-1312/9/6/648 en eng MDPI https://nora.nerc.ac.uk/id/eprint/529980/1/jmse-09-00648.pdf Darham, Syazani; Zahri, Khadijah Nabilah Mohd; Zulkharnain, Azham; Sabri, Suriana; Gomez-Fuentes, Claudio; Convey, Peter orcid:0000-0001-8497-9903 Khalil, Khalilah Abdul; Ahmad, Siti Aqlima. 2021 Statistical optimisation and kinetic studies of molybdenum reduction using a psychrotolerant marine bacteria isolated from Antarctica [in special issue: Research and Advance in Marine Biotechnology] Journal of Marine Science and Engineering, 9 (6), 648. 17, pp. https://doi.org/10.3390/jmse9060648 <https://doi.org/10.3390/jmse9060648> cc_by_4 CC-BY Publication - Article PeerReviewed 2021 ftnerc https://doi.org/10.3390/jmse9060648 2023-02-04T19:51:57Z The extensive industrial use of the heavy metal molybdenum (Mo) has led to an emerging global pollution with its traces that can even be found in Antarctica. In response, a reduction process that transforms hexamolybdate (Mo6+) to a less toxic compound, Mo-blue, using microorganisms provides a sustainable remediation approach. The aim of this study was to investigate the reduction of Mo by a psychrotolerant Antarctic marine bacterium, Marinomonas sp. strain AQ5-A9. Mo reduction was optimised using One-Factor-At-a-Time (OFAT) and Response Surface Methodology (RSM). Subsequently, Mo reduction kinetics were further studied. OFAT results showed that maximum Mo reduction occurred in culture media conditions of pH 6.0 and 50 ppt salinity at 15 °C, with initial sucrose, nitrogen and molybdate concentrations of 2.0%, 3.0 g/L and 10 mM, respectively. Further optimization using RSM identified improved optimum conditions of pH 6.0 and 47 ppt salinity at 16 °C, with initial sucrose, nitrogen and molybdate concentrations of 1.8%, 2.25 g/L and 16 mM, respectively. Investigation of the kinetics of Mo reduction revealed Aiba as the best-fitting model. The calculated Aiba coefficient of maximum Mo reduction rate (µmax) was 0.067 h−1. The data obtained support the potential use of marine bacteria in the bioremediation of Mo. Article in Journal/Newspaper Antarc* Antarctic Antarctica Natural Environment Research Council: NERC Open Research Archive Antarctic Journal of Marine Science and Engineering 9 6 648 |
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Open Polar |
collection |
Natural Environment Research Council: NERC Open Research Archive |
op_collection_id |
ftnerc |
language |
English |
description |
The extensive industrial use of the heavy metal molybdenum (Mo) has led to an emerging global pollution with its traces that can even be found in Antarctica. In response, a reduction process that transforms hexamolybdate (Mo6+) to a less toxic compound, Mo-blue, using microorganisms provides a sustainable remediation approach. The aim of this study was to investigate the reduction of Mo by a psychrotolerant Antarctic marine bacterium, Marinomonas sp. strain AQ5-A9. Mo reduction was optimised using One-Factor-At-a-Time (OFAT) and Response Surface Methodology (RSM). Subsequently, Mo reduction kinetics were further studied. OFAT results showed that maximum Mo reduction occurred in culture media conditions of pH 6.0 and 50 ppt salinity at 15 °C, with initial sucrose, nitrogen and molybdate concentrations of 2.0%, 3.0 g/L and 10 mM, respectively. Further optimization using RSM identified improved optimum conditions of pH 6.0 and 47 ppt salinity at 16 °C, with initial sucrose, nitrogen and molybdate concentrations of 1.8%, 2.25 g/L and 16 mM, respectively. Investigation of the kinetics of Mo reduction revealed Aiba as the best-fitting model. The calculated Aiba coefficient of maximum Mo reduction rate (µmax) was 0.067 h−1. The data obtained support the potential use of marine bacteria in the bioremediation of Mo. |
format |
Article in Journal/Newspaper |
author |
Darham, Syazani Zahri, Khadijah Nabilah Mohd Zulkharnain, Azham Sabri, Suriana Gomez-Fuentes, Claudio Convey, Peter Khalil, Khalilah Abdul Ahmad, Siti Aqlima |
spellingShingle |
Darham, Syazani Zahri, Khadijah Nabilah Mohd Zulkharnain, Azham Sabri, Suriana Gomez-Fuentes, Claudio Convey, Peter Khalil, Khalilah Abdul Ahmad, Siti Aqlima Statistical optimisation and kinetic studies of molybdenum reduction using a psychrotolerant marine bacteria isolated from Antarctica |
author_facet |
Darham, Syazani Zahri, Khadijah Nabilah Mohd Zulkharnain, Azham Sabri, Suriana Gomez-Fuentes, Claudio Convey, Peter Khalil, Khalilah Abdul Ahmad, Siti Aqlima |
author_sort |
Darham, Syazani |
title |
Statistical optimisation and kinetic studies of molybdenum reduction using a psychrotolerant marine bacteria isolated from Antarctica |
title_short |
Statistical optimisation and kinetic studies of molybdenum reduction using a psychrotolerant marine bacteria isolated from Antarctica |
title_full |
Statistical optimisation and kinetic studies of molybdenum reduction using a psychrotolerant marine bacteria isolated from Antarctica |
title_fullStr |
Statistical optimisation and kinetic studies of molybdenum reduction using a psychrotolerant marine bacteria isolated from Antarctica |
title_full_unstemmed |
Statistical optimisation and kinetic studies of molybdenum reduction using a psychrotolerant marine bacteria isolated from Antarctica |
title_sort |
statistical optimisation and kinetic studies of molybdenum reduction using a psychrotolerant marine bacteria isolated from antarctica |
publisher |
MDPI |
publishDate |
2021 |
url |
http://nora.nerc.ac.uk/id/eprint/529980/ https://nora.nerc.ac.uk/id/eprint/529980/1/jmse-09-00648.pdf https://www.mdpi.com/2077-1312/9/6/648 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_relation |
https://nora.nerc.ac.uk/id/eprint/529980/1/jmse-09-00648.pdf Darham, Syazani; Zahri, Khadijah Nabilah Mohd; Zulkharnain, Azham; Sabri, Suriana; Gomez-Fuentes, Claudio; Convey, Peter orcid:0000-0001-8497-9903 Khalil, Khalilah Abdul; Ahmad, Siti Aqlima. 2021 Statistical optimisation and kinetic studies of molybdenum reduction using a psychrotolerant marine bacteria isolated from Antarctica [in special issue: Research and Advance in Marine Biotechnology] Journal of Marine Science and Engineering, 9 (6), 648. 17, pp. https://doi.org/10.3390/jmse9060648 <https://doi.org/10.3390/jmse9060648> |
op_rights |
cc_by_4 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3390/jmse9060648 |
container_title |
Journal of Marine Science and Engineering |
container_volume |
9 |
container_issue |
6 |
container_start_page |
648 |
_version_ |
1766157363488751616 |