Liverworts frequently form mycothalli on Spitsbergen in the High Arctic
Mycothalli, symbioses between liverworts and soil fungi, have not previously been recorded in the Arctic. Here, 13 species of leafy liverwort from west Spitsbergen in the High Arctic are examined for the symbiosis using epifluorescence microscopy and sequencing of fungal ribosomal (r)RNA genes ampli...
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Norwegian Polar Institute
2021
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ftnerc:oai:nora.nerc.ac.uk:529827 2023-05-15T14:26:17+02:00 Liverworts frequently form mycothalli on Spitsbergen in the High Arctic Newsham, Kevin K. Goodall-Copestake, William P. 2021-09-07 text http://nora.nerc.ac.uk/id/eprint/529827/ https://nora.nerc.ac.uk/id/eprint/529827/1/7727-Article%20Text-50937-1-10-20210908.pdf https://polarresearch.net/index.php/polar/article/view/7727 en eng Norwegian Polar Institute https://nora.nerc.ac.uk/id/eprint/529827/1/7727-Article%20Text-50937-1-10-20210908.pdf Newsham, Kevin K. orcid:0000-0002-9108-0936 Goodall-Copestake, William P. orcid:0000-0003-3586-9091 . 2021 Liverworts frequently form mycothalli on Spitsbergen in the High Arctic. Polar Research, 40, 7727. 13, pp. https://doi.org/10.33265/polar.v40.7727 <https://doi.org/10.33265/polar.v40.7727> cc_by_4 CC-BY Publication - Article PeerReviewed 2021 ftnerc https://doi.org/10.33265/polar.v40.7727 2023-02-04T19:51:49Z Mycothalli, symbioses between liverworts and soil fungi, have not previously been recorded in the Arctic. Here, 13 species of leafy liverwort from west Spitsbergen in the High Arctic are examined for the symbiosis using epifluorescence microscopy and sequencing of fungal ribosomal (r)RNA genes amplified from plant tissues. Microscopy showed that intracellular hyphal coils, key indicators of the symbiosis, were frequent (>40% stem length colonized) in nine species of liverwort in the families Anastrophyllaceae, Lophoziaceae, Cephaloziellaceae, Cephaloziaceae and Scapaniaceae, with hyphae occurring frequently (>40% cells occupied) in the rhizoids of 10 species in the same families. Dark septate hyphae, apparently formed by ascomycetes, were frequent on the stems of members of the Anastrophyllaceae, Cephaloziellaceae and Cephaloziaceae, and typically those growing on acidic mine tailings. Sequencing of fungal rRNA genes showed the presence of nine distinct groups (based on a 3% cut-off for ITS sequence divergence) of the basidiomycete Serendipita in the Anastrophyllaceae and Lophoziaceae, with ordinations and correlative analyses showing the presence of the genus to be positively associated with the frequency of hyphal coils, the occurrence of which was positively associated with edaphic factors (soil δ15N value and concentrations of moisture, nitrogen, carbon and organic matter). We propose that the frequency of mycothalli in leafy liverworts on west Spitsbergen, which is an order of magnitude higher than at lower latitudes, may arise from benefits conferred by mycobionts on their hosts in the harsh environment of the High Arctic. Article in Journal/Newspaper Arctic Arctic Polar Research Spitsbergen Natural Environment Research Council: NERC Open Research Archive Arctic Polar Research 40 |
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Open Polar |
collection |
Natural Environment Research Council: NERC Open Research Archive |
op_collection_id |
ftnerc |
language |
English |
description |
Mycothalli, symbioses between liverworts and soil fungi, have not previously been recorded in the Arctic. Here, 13 species of leafy liverwort from west Spitsbergen in the High Arctic are examined for the symbiosis using epifluorescence microscopy and sequencing of fungal ribosomal (r)RNA genes amplified from plant tissues. Microscopy showed that intracellular hyphal coils, key indicators of the symbiosis, were frequent (>40% stem length colonized) in nine species of liverwort in the families Anastrophyllaceae, Lophoziaceae, Cephaloziellaceae, Cephaloziaceae and Scapaniaceae, with hyphae occurring frequently (>40% cells occupied) in the rhizoids of 10 species in the same families. Dark septate hyphae, apparently formed by ascomycetes, were frequent on the stems of members of the Anastrophyllaceae, Cephaloziellaceae and Cephaloziaceae, and typically those growing on acidic mine tailings. Sequencing of fungal rRNA genes showed the presence of nine distinct groups (based on a 3% cut-off for ITS sequence divergence) of the basidiomycete Serendipita in the Anastrophyllaceae and Lophoziaceae, with ordinations and correlative analyses showing the presence of the genus to be positively associated with the frequency of hyphal coils, the occurrence of which was positively associated with edaphic factors (soil δ15N value and concentrations of moisture, nitrogen, carbon and organic matter). We propose that the frequency of mycothalli in leafy liverworts on west Spitsbergen, which is an order of magnitude higher than at lower latitudes, may arise from benefits conferred by mycobionts on their hosts in the harsh environment of the High Arctic. |
format |
Article in Journal/Newspaper |
author |
Newsham, Kevin K. Goodall-Copestake, William P. |
spellingShingle |
Newsham, Kevin K. Goodall-Copestake, William P. Liverworts frequently form mycothalli on Spitsbergen in the High Arctic |
author_facet |
Newsham, Kevin K. Goodall-Copestake, William P. |
author_sort |
Newsham, Kevin K. |
title |
Liverworts frequently form mycothalli on Spitsbergen in the High Arctic |
title_short |
Liverworts frequently form mycothalli on Spitsbergen in the High Arctic |
title_full |
Liverworts frequently form mycothalli on Spitsbergen in the High Arctic |
title_fullStr |
Liverworts frequently form mycothalli on Spitsbergen in the High Arctic |
title_full_unstemmed |
Liverworts frequently form mycothalli on Spitsbergen in the High Arctic |
title_sort |
liverworts frequently form mycothalli on spitsbergen in the high arctic |
publisher |
Norwegian Polar Institute |
publishDate |
2021 |
url |
http://nora.nerc.ac.uk/id/eprint/529827/ https://nora.nerc.ac.uk/id/eprint/529827/1/7727-Article%20Text-50937-1-10-20210908.pdf https://polarresearch.net/index.php/polar/article/view/7727 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Arctic Polar Research Spitsbergen |
genre_facet |
Arctic Arctic Polar Research Spitsbergen |
op_relation |
https://nora.nerc.ac.uk/id/eprint/529827/1/7727-Article%20Text-50937-1-10-20210908.pdf Newsham, Kevin K. orcid:0000-0002-9108-0936 Goodall-Copestake, William P. orcid:0000-0003-3586-9091 . 2021 Liverworts frequently form mycothalli on Spitsbergen in the High Arctic. Polar Research, 40, 7727. 13, pp. https://doi.org/10.33265/polar.v40.7727 <https://doi.org/10.33265/polar.v40.7727> |
op_rights |
cc_by_4 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.33265/polar.v40.7727 |
container_title |
Polar Research |
container_volume |
40 |
_version_ |
1766298780438626304 |