Growth optimisation and kinetic profiling of diesel biodegradation by a cold‒adapted microbial consortium isolated from Trinity Peninsula, Antarctica
Pollution associated with petrogenic hydrocarbons is increasing in Antarctica due to a combination of increasing human activity and the continent’s unforgiving environmental conditions. The current study focuses on the ability of a cold-adapted crude microbial consortium (BS24), isolated from soil o...
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Online Access: | http://nora.nerc.ac.uk/id/eprint/529735/ https://nora.nerc.ac.uk/id/eprint/529735/1/biology-10-00493.pdf https://www.mdpi.com/2079-7737/10/6/493 |
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ftnerc:oai:nora.nerc.ac.uk:529735 2023-05-15T13:41:45+02:00 Growth optimisation and kinetic profiling of diesel biodegradation by a cold‒adapted microbial consortium isolated from Trinity Peninsula, Antarctica Roslee, Ahmad Fareez Ahmad Gomez-Fuentes, Claudio Zakaria, Nur Nadhirah Shaharudin, Nor Azmi Zulkharnain, Azham Khalil, Khalilah Abdul Convey, Peter Ahmad, Siti Aqlima 2021-06-02 text http://nora.nerc.ac.uk/id/eprint/529735/ https://nora.nerc.ac.uk/id/eprint/529735/1/biology-10-00493.pdf https://www.mdpi.com/2079-7737/10/6/493 en eng MDPI https://nora.nerc.ac.uk/id/eprint/529735/1/biology-10-00493.pdf Roslee, Ahmad Fareez Ahmad; Gomez-Fuentes, Claudio; Zakaria, Nur Nadhirah; Shaharudin, Nor Azmi; Zulkharnain, Azham; Khalil, Khalilah Abdul; Convey, Peter orcid:0000-0001-8497-9903 Ahmad, Siti Aqlima. 2021 Growth optimisation and kinetic profiling of diesel biodegradation by a cold‒adapted microbial consortium isolated from Trinity Peninsula, Antarctica. Biology, 10 (6), 493. 21, pp. https://doi.org/10.3390/biology10060493 <https://doi.org/10.3390/biology10060493> cc_by_4 CC-BY Publication - Article PeerReviewed 2021 ftnerc https://doi.org/10.3390/biology10060493 2023-02-04T19:51:49Z Pollution associated with petrogenic hydrocarbons is increasing in Antarctica due to a combination of increasing human activity and the continent’s unforgiving environmental conditions. The current study focuses on the ability of a cold-adapted crude microbial consortium (BS24), isolated from soil on the north-west Antarctic Peninsula, to metabolise diesel fuel as the sole carbon source in a shake-flask setting. Factors expected to influence the efficiency of diesel biodegradation, namely temperature, initial diesel concentration, nitrogen source type and concentration, salinity and pH were studied. Consortium BS24 displayed optimal cell growth and diesel degradation activity at 1.0% NaCl, pH 7.5, 0.5 g/L NH4Cl and 2.0% v/v initial diesel concentration during one-factor-at-a-time (OFAT) analyses. The consortium was psychrotolerant based on the optimum growth temperature of 10‒15 °C. In conventionally optimised media, the highest total petroleum hydrocarbons (TPH) mineralisation was 85% over a 7-day incubation. Further optimisation of conditions predicted through statistical response-surface methodology (RSM) (1.0% NaCl, pH 7.25, 0.75 g/L NH4Cl, 12.5 °C and 1.75% v/v initial diesel concentration) boosted mineralisation to 95% over a 7-day incubation. A Tessier secondary model best described the growth pattern of BS24 in diesel-enriched medium, with maximum specific growth rate, μmax, substrate inhibition constant, Ki and half saturation constant, Ks, being 0.9996 h−1, 1.356% v/v and 1.238% v/v, respectively. The data obtained suggest the potential of microbial consortia such as BS24 in bioremediation applications in low-temperature diesel-polluted soils. Article in Journal/Newspaper Antarc* Antarctic Antarctic Peninsula Antarctica Natural Environment Research Council: NERC Open Research Archive Antarctic Antarctic Peninsula Trinity Peninsula ENVELOPE(-58.000,-58.000,-63.500,-63.500) Biology 10 6 493 |
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Open Polar |
collection |
Natural Environment Research Council: NERC Open Research Archive |
op_collection_id |
ftnerc |
language |
English |
description |
Pollution associated with petrogenic hydrocarbons is increasing in Antarctica due to a combination of increasing human activity and the continent’s unforgiving environmental conditions. The current study focuses on the ability of a cold-adapted crude microbial consortium (BS24), isolated from soil on the north-west Antarctic Peninsula, to metabolise diesel fuel as the sole carbon source in a shake-flask setting. Factors expected to influence the efficiency of diesel biodegradation, namely temperature, initial diesel concentration, nitrogen source type and concentration, salinity and pH were studied. Consortium BS24 displayed optimal cell growth and diesel degradation activity at 1.0% NaCl, pH 7.5, 0.5 g/L NH4Cl and 2.0% v/v initial diesel concentration during one-factor-at-a-time (OFAT) analyses. The consortium was psychrotolerant based on the optimum growth temperature of 10‒15 °C. In conventionally optimised media, the highest total petroleum hydrocarbons (TPH) mineralisation was 85% over a 7-day incubation. Further optimisation of conditions predicted through statistical response-surface methodology (RSM) (1.0% NaCl, pH 7.25, 0.75 g/L NH4Cl, 12.5 °C and 1.75% v/v initial diesel concentration) boosted mineralisation to 95% over a 7-day incubation. A Tessier secondary model best described the growth pattern of BS24 in diesel-enriched medium, with maximum specific growth rate, μmax, substrate inhibition constant, Ki and half saturation constant, Ks, being 0.9996 h−1, 1.356% v/v and 1.238% v/v, respectively. The data obtained suggest the potential of microbial consortia such as BS24 in bioremediation applications in low-temperature diesel-polluted soils. |
format |
Article in Journal/Newspaper |
author |
Roslee, Ahmad Fareez Ahmad Gomez-Fuentes, Claudio Zakaria, Nur Nadhirah Shaharudin, Nor Azmi Zulkharnain, Azham Khalil, Khalilah Abdul Convey, Peter Ahmad, Siti Aqlima |
spellingShingle |
Roslee, Ahmad Fareez Ahmad Gomez-Fuentes, Claudio Zakaria, Nur Nadhirah Shaharudin, Nor Azmi Zulkharnain, Azham Khalil, Khalilah Abdul Convey, Peter Ahmad, Siti Aqlima Growth optimisation and kinetic profiling of diesel biodegradation by a cold‒adapted microbial consortium isolated from Trinity Peninsula, Antarctica |
author_facet |
Roslee, Ahmad Fareez Ahmad Gomez-Fuentes, Claudio Zakaria, Nur Nadhirah Shaharudin, Nor Azmi Zulkharnain, Azham Khalil, Khalilah Abdul Convey, Peter Ahmad, Siti Aqlima |
author_sort |
Roslee, Ahmad Fareez Ahmad |
title |
Growth optimisation and kinetic profiling of diesel biodegradation by a cold‒adapted microbial consortium isolated from Trinity Peninsula, Antarctica |
title_short |
Growth optimisation and kinetic profiling of diesel biodegradation by a cold‒adapted microbial consortium isolated from Trinity Peninsula, Antarctica |
title_full |
Growth optimisation and kinetic profiling of diesel biodegradation by a cold‒adapted microbial consortium isolated from Trinity Peninsula, Antarctica |
title_fullStr |
Growth optimisation and kinetic profiling of diesel biodegradation by a cold‒adapted microbial consortium isolated from Trinity Peninsula, Antarctica |
title_full_unstemmed |
Growth optimisation and kinetic profiling of diesel biodegradation by a cold‒adapted microbial consortium isolated from Trinity Peninsula, Antarctica |
title_sort |
growth optimisation and kinetic profiling of diesel biodegradation by a cold‒adapted microbial consortium isolated from trinity peninsula, antarctica |
publisher |
MDPI |
publishDate |
2021 |
url |
http://nora.nerc.ac.uk/id/eprint/529735/ https://nora.nerc.ac.uk/id/eprint/529735/1/biology-10-00493.pdf https://www.mdpi.com/2079-7737/10/6/493 |
long_lat |
ENVELOPE(-58.000,-58.000,-63.500,-63.500) |
geographic |
Antarctic Antarctic Peninsula Trinity Peninsula |
geographic_facet |
Antarctic Antarctic Peninsula Trinity Peninsula |
genre |
Antarc* Antarctic Antarctic Peninsula Antarctica |
genre_facet |
Antarc* Antarctic Antarctic Peninsula Antarctica |
op_relation |
https://nora.nerc.ac.uk/id/eprint/529735/1/biology-10-00493.pdf Roslee, Ahmad Fareez Ahmad; Gomez-Fuentes, Claudio; Zakaria, Nur Nadhirah; Shaharudin, Nor Azmi; Zulkharnain, Azham; Khalil, Khalilah Abdul; Convey, Peter orcid:0000-0001-8497-9903 Ahmad, Siti Aqlima. 2021 Growth optimisation and kinetic profiling of diesel biodegradation by a cold‒adapted microbial consortium isolated from Trinity Peninsula, Antarctica. Biology, 10 (6), 493. 21, pp. https://doi.org/10.3390/biology10060493 <https://doi.org/10.3390/biology10060493> |
op_rights |
cc_by_4 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3390/biology10060493 |
container_title |
Biology |
container_volume |
10 |
container_issue |
6 |
container_start_page |
493 |
_version_ |
1766157294178926592 |