Invasion, isolation and evolution shape population genetic structure in Campanula rotundifolia
The distribution and genetic structure of most plant species in Britain and Ireland bear the imprint of the last ice age. These patterns were largely shaped by random processes during recolonization but, in angiosperms, whole-genome duplication may also have been important. We investigate the distri...
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ftnerc:oai:nora.nerc.ac.uk:527837 2023-05-15T15:48:42+02:00 Invasion, isolation and evolution shape population genetic structure in Campanula rotundifolia Wilson, Julia Perry, Annika Shepherd, Jessica R. Durán-Castillo, Mario Jeffree, Christopher E. Cavers, Stephen 2020-04 text http://nora.nerc.ac.uk/id/eprint/527837/ https://nora.nerc.ac.uk/id/eprint/527837/1/N527837JA.pdf https://doi.org/10.1093/aobpla/plaa011 en eng Oxford University Press https://nora.nerc.ac.uk/id/eprint/527837/1/N527837JA.pdf Wilson, Julia; Perry, Annika; Shepherd, Jessica R.; Durán-Castillo, Mario; Jeffree, Christopher E.; Cavers, Stephen. 2020 Invasion, isolation and evolution shape population genetic structure in Campanula rotundifolia. AoB Plants, 12 (2), plaa011. 14, pp. https://doi.org/10.1093/aobpla/plaa011 <https://doi.org/10.1093/aobpla/plaa011> cc_by_4 CC-BY Ecology and Environment Publication - Article PeerReviewed 2020 ftnerc https://doi.org/10.1093/aobpla/plaa011 2023-02-04T19:50:43Z The distribution and genetic structure of most plant species in Britain and Ireland bear the imprint of the last ice age. These patterns were largely shaped by random processes during recolonization but, in angiosperms, whole-genome duplication may also have been important. We investigate the distribution of cytotypes of Campanula rotundifolia, considering DNA variation, postglacial colonization, environmental partitioning and reproductive barriers. Cytotypes and genome size variation from across the species’ range were determined by flow cytometry and genetic variation was assessed using cpDNA markers. A common garden study examined growth and flowering phenology of tetraploid, pentaploid and hexaploid cytotypes and simulated a contact zone for investigation of reproductive barriers. Irish populations were entirely hexaploid. In Britain, hexaploids occurred mostly in western coastal populations which were allopatric with tetraploids, and in occasional sympatric inland populations. Chloroplast markers resolved distinct genetic groups, related to cytotype and geographically segregated; allopatric hexaploids were distinct from tetraploids, whereas sympatric hexaploids were not. Genome downsizing occurred between cytotypes. Progeny of open-pollinated clones from the contact zone showed that maternal tetraploids rarely produced progeny of other cytotypes, whereas the progeny of maternal hexaploids varied, with frequent pentaploids and aneuploids. The presence of distinctive hexaploid chloroplast types in Ireland, Scottish islands and western mainland Britain indicates that its establishment preceded separation of these land masses by sea-level rise c. 16 000 years BP. This group did not originate from British tetraploids and probably diverged before postglacial invasion from mainland Europe. The combination of cytotype, molecular, contact zone and common garden data shows an overall pattern reflecting postglacial colonization events, now maintained by geographic separation, together with more recent occasional local ... Article in Journal/Newspaper Campanula rotundifolia Natural Environment Research Council: NERC Open Research Archive AoB PLANTS 12 2 |
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Open Polar |
collection |
Natural Environment Research Council: NERC Open Research Archive |
op_collection_id |
ftnerc |
language |
English |
topic |
Ecology and Environment |
spellingShingle |
Ecology and Environment Wilson, Julia Perry, Annika Shepherd, Jessica R. Durán-Castillo, Mario Jeffree, Christopher E. Cavers, Stephen Invasion, isolation and evolution shape population genetic structure in Campanula rotundifolia |
topic_facet |
Ecology and Environment |
description |
The distribution and genetic structure of most plant species in Britain and Ireland bear the imprint of the last ice age. These patterns were largely shaped by random processes during recolonization but, in angiosperms, whole-genome duplication may also have been important. We investigate the distribution of cytotypes of Campanula rotundifolia, considering DNA variation, postglacial colonization, environmental partitioning and reproductive barriers. Cytotypes and genome size variation from across the species’ range were determined by flow cytometry and genetic variation was assessed using cpDNA markers. A common garden study examined growth and flowering phenology of tetraploid, pentaploid and hexaploid cytotypes and simulated a contact zone for investigation of reproductive barriers. Irish populations were entirely hexaploid. In Britain, hexaploids occurred mostly in western coastal populations which were allopatric with tetraploids, and in occasional sympatric inland populations. Chloroplast markers resolved distinct genetic groups, related to cytotype and geographically segregated; allopatric hexaploids were distinct from tetraploids, whereas sympatric hexaploids were not. Genome downsizing occurred between cytotypes. Progeny of open-pollinated clones from the contact zone showed that maternal tetraploids rarely produced progeny of other cytotypes, whereas the progeny of maternal hexaploids varied, with frequent pentaploids and aneuploids. The presence of distinctive hexaploid chloroplast types in Ireland, Scottish islands and western mainland Britain indicates that its establishment preceded separation of these land masses by sea-level rise c. 16 000 years BP. This group did not originate from British tetraploids and probably diverged before postglacial invasion from mainland Europe. The combination of cytotype, molecular, contact zone and common garden data shows an overall pattern reflecting postglacial colonization events, now maintained by geographic separation, together with more recent occasional local ... |
format |
Article in Journal/Newspaper |
author |
Wilson, Julia Perry, Annika Shepherd, Jessica R. Durán-Castillo, Mario Jeffree, Christopher E. Cavers, Stephen |
author_facet |
Wilson, Julia Perry, Annika Shepherd, Jessica R. Durán-Castillo, Mario Jeffree, Christopher E. Cavers, Stephen |
author_sort |
Wilson, Julia |
title |
Invasion, isolation and evolution shape population genetic structure in Campanula rotundifolia |
title_short |
Invasion, isolation and evolution shape population genetic structure in Campanula rotundifolia |
title_full |
Invasion, isolation and evolution shape population genetic structure in Campanula rotundifolia |
title_fullStr |
Invasion, isolation and evolution shape population genetic structure in Campanula rotundifolia |
title_full_unstemmed |
Invasion, isolation and evolution shape population genetic structure in Campanula rotundifolia |
title_sort |
invasion, isolation and evolution shape population genetic structure in campanula rotundifolia |
publisher |
Oxford University Press |
publishDate |
2020 |
url |
http://nora.nerc.ac.uk/id/eprint/527837/ https://nora.nerc.ac.uk/id/eprint/527837/1/N527837JA.pdf https://doi.org/10.1093/aobpla/plaa011 |
genre |
Campanula rotundifolia |
genre_facet |
Campanula rotundifolia |
op_relation |
https://nora.nerc.ac.uk/id/eprint/527837/1/N527837JA.pdf Wilson, Julia; Perry, Annika; Shepherd, Jessica R.; Durán-Castillo, Mario; Jeffree, Christopher E.; Cavers, Stephen. 2020 Invasion, isolation and evolution shape population genetic structure in Campanula rotundifolia. AoB Plants, 12 (2), plaa011. 14, pp. https://doi.org/10.1093/aobpla/plaa011 <https://doi.org/10.1093/aobpla/plaa011> |
op_rights |
cc_by_4 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1093/aobpla/plaa011 |
container_title |
AoB PLANTS |
container_volume |
12 |
container_issue |
2 |
_version_ |
1766383820244779008 |