Diversity and ecology of Chlorophyta (Viridiplantae) assemblages in protected and non-protected sites in Deception Island (Antarctica, South Shetland Islands) assessed using an NGS approach
Assessment of the diversity of algal assemblages in Antarctica has until now largely relied on traditional microbiological culture approaches. Here we used DNA metabarcoding through high-throughput sequencing (HTS) to assess the uncultured algal diversity at two sites on Deception Island, Antarctica...
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2021
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Online Access: | http://nora.nerc.ac.uk/id/eprint/527057/ https://link.springer.com/article/10.1007/s00248-020-01584-9 |
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ftnerc:oai:nora.nerc.ac.uk:527057 2023-05-15T13:41:45+02:00 Diversity and ecology of Chlorophyta (Viridiplantae) assemblages in protected and non-protected sites in Deception Island (Antarctica, South Shetland Islands) assessed using an NGS approach Câmara, Paulo E.A.S. Carvalho-Silva, Micheline Pinto, Otávio H.B. Henriques, Diego Knop da Silva, Thamar Holanda Stech, Michael Pellizzari, Franciane Convey, Peter Rosa, Luiz Henrique 2021-02 http://nora.nerc.ac.uk/id/eprint/527057/ https://link.springer.com/article/10.1007/s00248-020-01584-9 unknown Springer Câmara, Paulo E.A.S.; Carvalho-Silva, Micheline; Pinto, Otávio H.B.; Henriques, Diego Knop; da Silva, Thamar Holanda; Stech, Michael; Pellizzari, Franciane; Convey, Peter orcid:0000-0001-8497-9903 Rosa, Luiz Henrique. 2021 Diversity and ecology of Chlorophyta (Viridiplantae) assemblages in protected and non-protected sites in Deception Island (Antarctica, South Shetland Islands) assessed using an NGS approach. Microbial Ecology, 81. 323-334. https://doi.org/10.1007/s00248-020-01584-9 <https://doi.org/10.1007/s00248-020-01584-9> Ecology and Environment Botany Publication - Article PeerReviewed 2021 ftnerc https://doi.org/10.1007/s00248-020-01584-9 2023-02-04T19:50:16Z Assessment of the diversity of algal assemblages in Antarctica has until now largely relied on traditional microbiological culture approaches. Here we used DNA metabarcoding through high-throughput sequencing (HTS) to assess the uncultured algal diversity at two sites on Deception Island, Antarctica. The first was a relatively undisturbed site within an Antarctic Specially Protected Area (ASPA 140), and the second was a site heavily impacted by human visitation, the Whalers Bay historic site. We detected 65 distinct algal taxa, 50 from within ASPA 140 and 61 from Whalers Bay. Of these taxa, 46 were common to both sites, and 19 only occurred at one site. Algal richness was about six times greater than reported in previous studies using culture methods. A high proportion of DNA reads obtained was assigned to the highly invasive species Caulerpa webbiana at Whalers Bay, and the potentially pathogenic genus Desmodesmus was found at both sites. Our data demonstrate that important differences exist between these two protected and human-impacted sites on Deception Island in terms of algal diversity, richness, and abundance. The South Shetland Islands have experienced considerable effects of climate change in recent decades, while warming through geothermal activity on Deception Island itself makes this island one of the most vulnerable to colonization by non-native species. The detection of DNA of non-native taxa highlights concerns about how human impacts, which take place primarily through tourism and national research operations, may influence future biological colonization processes in Antarctica. Article in Journal/Newspaper Antarc* Antarctic Antarctica Deception Island South Shetland Islands Natural Environment Research Council: NERC Open Research Archive Antarctic South Shetland Islands Deception Island ENVELOPE(-60.633,-60.633,-62.950,-62.950) Whalers Bay ENVELOPE(69.917,69.917,-49.100,-49.100) Microbial Ecology 81 2 323 334 |
institution |
Open Polar |
collection |
Natural Environment Research Council: NERC Open Research Archive |
op_collection_id |
ftnerc |
language |
unknown |
topic |
Ecology and Environment Botany |
spellingShingle |
Ecology and Environment Botany Câmara, Paulo E.A.S. Carvalho-Silva, Micheline Pinto, Otávio H.B. Henriques, Diego Knop da Silva, Thamar Holanda Stech, Michael Pellizzari, Franciane Convey, Peter Rosa, Luiz Henrique Diversity and ecology of Chlorophyta (Viridiplantae) assemblages in protected and non-protected sites in Deception Island (Antarctica, South Shetland Islands) assessed using an NGS approach |
topic_facet |
Ecology and Environment Botany |
description |
Assessment of the diversity of algal assemblages in Antarctica has until now largely relied on traditional microbiological culture approaches. Here we used DNA metabarcoding through high-throughput sequencing (HTS) to assess the uncultured algal diversity at two sites on Deception Island, Antarctica. The first was a relatively undisturbed site within an Antarctic Specially Protected Area (ASPA 140), and the second was a site heavily impacted by human visitation, the Whalers Bay historic site. We detected 65 distinct algal taxa, 50 from within ASPA 140 and 61 from Whalers Bay. Of these taxa, 46 were common to both sites, and 19 only occurred at one site. Algal richness was about six times greater than reported in previous studies using culture methods. A high proportion of DNA reads obtained was assigned to the highly invasive species Caulerpa webbiana at Whalers Bay, and the potentially pathogenic genus Desmodesmus was found at both sites. Our data demonstrate that important differences exist between these two protected and human-impacted sites on Deception Island in terms of algal diversity, richness, and abundance. The South Shetland Islands have experienced considerable effects of climate change in recent decades, while warming through geothermal activity on Deception Island itself makes this island one of the most vulnerable to colonization by non-native species. The detection of DNA of non-native taxa highlights concerns about how human impacts, which take place primarily through tourism and national research operations, may influence future biological colonization processes in Antarctica. |
format |
Article in Journal/Newspaper |
author |
Câmara, Paulo E.A.S. Carvalho-Silva, Micheline Pinto, Otávio H.B. Henriques, Diego Knop da Silva, Thamar Holanda Stech, Michael Pellizzari, Franciane Convey, Peter Rosa, Luiz Henrique |
author_facet |
Câmara, Paulo E.A.S. Carvalho-Silva, Micheline Pinto, Otávio H.B. Henriques, Diego Knop da Silva, Thamar Holanda Stech, Michael Pellizzari, Franciane Convey, Peter Rosa, Luiz Henrique |
author_sort |
Câmara, Paulo E.A.S. |
title |
Diversity and ecology of Chlorophyta (Viridiplantae) assemblages in protected and non-protected sites in Deception Island (Antarctica, South Shetland Islands) assessed using an NGS approach |
title_short |
Diversity and ecology of Chlorophyta (Viridiplantae) assemblages in protected and non-protected sites in Deception Island (Antarctica, South Shetland Islands) assessed using an NGS approach |
title_full |
Diversity and ecology of Chlorophyta (Viridiplantae) assemblages in protected and non-protected sites in Deception Island (Antarctica, South Shetland Islands) assessed using an NGS approach |
title_fullStr |
Diversity and ecology of Chlorophyta (Viridiplantae) assemblages in protected and non-protected sites in Deception Island (Antarctica, South Shetland Islands) assessed using an NGS approach |
title_full_unstemmed |
Diversity and ecology of Chlorophyta (Viridiplantae) assemblages in protected and non-protected sites in Deception Island (Antarctica, South Shetland Islands) assessed using an NGS approach |
title_sort |
diversity and ecology of chlorophyta (viridiplantae) assemblages in protected and non-protected sites in deception island (antarctica, south shetland islands) assessed using an ngs approach |
publisher |
Springer |
publishDate |
2021 |
url |
http://nora.nerc.ac.uk/id/eprint/527057/ https://link.springer.com/article/10.1007/s00248-020-01584-9 |
long_lat |
ENVELOPE(-60.633,-60.633,-62.950,-62.950) ENVELOPE(69.917,69.917,-49.100,-49.100) |
geographic |
Antarctic South Shetland Islands Deception Island Whalers Bay |
geographic_facet |
Antarctic South Shetland Islands Deception Island Whalers Bay |
genre |
Antarc* Antarctic Antarctica Deception Island South Shetland Islands |
genre_facet |
Antarc* Antarctic Antarctica Deception Island South Shetland Islands |
op_relation |
Câmara, Paulo E.A.S.; Carvalho-Silva, Micheline; Pinto, Otávio H.B.; Henriques, Diego Knop; da Silva, Thamar Holanda; Stech, Michael; Pellizzari, Franciane; Convey, Peter orcid:0000-0001-8497-9903 Rosa, Luiz Henrique. 2021 Diversity and ecology of Chlorophyta (Viridiplantae) assemblages in protected and non-protected sites in Deception Island (Antarctica, South Shetland Islands) assessed using an NGS approach. Microbial Ecology, 81. 323-334. https://doi.org/10.1007/s00248-020-01584-9 <https://doi.org/10.1007/s00248-020-01584-9> |
op_doi |
https://doi.org/10.1007/s00248-020-01584-9 |
container_title |
Microbial Ecology |
container_volume |
81 |
container_issue |
2 |
container_start_page |
323 |
op_container_end_page |
334 |
_version_ |
1766156734580129792 |