Biosurfactant production and growth kinetics studies of the waste canola oil-degrading bacterium Rhodococcus erythropolis AQ5-07 from Antarctica
With the progressive increase in human activities in the Antarctic region, the possibility of domestic oil spillage also increases. Developing means for the removal of oils, such as canola oil, from the environment and waste “grey” water using biological approaches is therefore desirable, since the...
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ftnerc:oai:nora.nerc.ac.uk:526346 2023-05-15T13:41:44+02:00 Biosurfactant production and growth kinetics studies of the waste canola oil-degrading bacterium Rhodococcus erythropolis AQ5-07 from Antarctica Ibrahim, Salihu Khalil, Khalilah Abdul Zahri, Khadijah Nabilah Mohd Gomez-Fuentes, Claudio Convey, Peter Zulkarnain, Azham Sabri, Suriana Alias, Siti Aisyah González-Rocha, Gerardo Ahmad, Siti Aqlima 2020-08-26 text http://nora.nerc.ac.uk/id/eprint/526346/ https://nora.nerc.ac.uk/id/eprint/526346/1/molecules-25-03878-v2.pdf https://www.mdpi.com/1420-3049/25/17/3878 en eng MDPI https://nora.nerc.ac.uk/id/eprint/526346/1/molecules-25-03878-v2.pdf Ibrahim, Salihu; Khalil, Khalilah Abdul; Zahri, Khadijah Nabilah Mohd; Gomez-Fuentes, Claudio; Convey, Peter orcid:0000-0001-8497-9903 Zulkarnain, Azham; Sabri, Suriana; Alias, Siti Aisyah; González-Rocha, Gerardo; Ahmad, Siti Aqlima. 2020 Biosurfactant production and growth kinetics studies of the waste canola oil-degrading bacterium Rhodococcus erythropolis AQ5-07 from Antarctica. Molecules, 25 (17), 3878. https://doi.org/10.3390/molecules25173878 <https://doi.org/10.3390/molecules25173878> cc_by_4 CC-BY Publication - Article PeerReviewed 2020 ftnerc https://doi.org/10.3390/molecules25173878 2023-02-04T19:49:55Z With the progressive increase in human activities in the Antarctic region, the possibility of domestic oil spillage also increases. Developing means for the removal of oils, such as canola oil, from the environment and waste “grey” water using biological approaches is therefore desirable, since the thermal process of oil degradation is expensive and ineffective. Thus, in this study an indigenous cold-adapted Antarctic soil bacterium, Rhodococcus erythropolis strain AQ5-07, was screened for biosurfactant production ability using the multiple approaches of blood haemolysis, surface tension, emulsification index, oil spreading, drop collapse and “MATH” assay for cellular hydrophobicity. The growth kinetics of the bacterium containing different canola oil concentration was studied. The strain showed β-haemolysis on blood agar with a high emulsification index and low surface tension value of 91.5% and 25.14 mN/m, respectively. Of the models tested, the Haldane model provided the best description of the growth kinetics, although several models were similar in performance. Parameters obtained from the modelling were the maximum specific growth rate (qmax), concentration of substrate at the half maximum specific growth rate, Ks% (v/v) and the inhibition constant Ki% (v/v), with values of 0.142 h−1, 7.743% (v/v) and 0.399% (v/v), respectively. These biological coefficients are useful in predicting growth conditions for batch studies, and also relevant to “in field” bioremediation strategies where the concentration of oil might need to be diluted to non-toxic levels prior to remediation. Biosurfactants can also have application in enhanced oil recovery (EOR) under different environmental conditions. Article in Journal/Newspaper Antarc* Antarctic Antarctica Natural Environment Research Council: NERC Open Research Archive Antarctic The Antarctic Molecules 25 17 3878 |
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Open Polar |
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Natural Environment Research Council: NERC Open Research Archive |
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ftnerc |
language |
English |
description |
With the progressive increase in human activities in the Antarctic region, the possibility of domestic oil spillage also increases. Developing means for the removal of oils, such as canola oil, from the environment and waste “grey” water using biological approaches is therefore desirable, since the thermal process of oil degradation is expensive and ineffective. Thus, in this study an indigenous cold-adapted Antarctic soil bacterium, Rhodococcus erythropolis strain AQ5-07, was screened for biosurfactant production ability using the multiple approaches of blood haemolysis, surface tension, emulsification index, oil spreading, drop collapse and “MATH” assay for cellular hydrophobicity. The growth kinetics of the bacterium containing different canola oil concentration was studied. The strain showed β-haemolysis on blood agar with a high emulsification index and low surface tension value of 91.5% and 25.14 mN/m, respectively. Of the models tested, the Haldane model provided the best description of the growth kinetics, although several models were similar in performance. Parameters obtained from the modelling were the maximum specific growth rate (qmax), concentration of substrate at the half maximum specific growth rate, Ks% (v/v) and the inhibition constant Ki% (v/v), with values of 0.142 h−1, 7.743% (v/v) and 0.399% (v/v), respectively. These biological coefficients are useful in predicting growth conditions for batch studies, and also relevant to “in field” bioremediation strategies where the concentration of oil might need to be diluted to non-toxic levels prior to remediation. Biosurfactants can also have application in enhanced oil recovery (EOR) under different environmental conditions. |
format |
Article in Journal/Newspaper |
author |
Ibrahim, Salihu Khalil, Khalilah Abdul Zahri, Khadijah Nabilah Mohd Gomez-Fuentes, Claudio Convey, Peter Zulkarnain, Azham Sabri, Suriana Alias, Siti Aisyah González-Rocha, Gerardo Ahmad, Siti Aqlima |
spellingShingle |
Ibrahim, Salihu Khalil, Khalilah Abdul Zahri, Khadijah Nabilah Mohd Gomez-Fuentes, Claudio Convey, Peter Zulkarnain, Azham Sabri, Suriana Alias, Siti Aisyah González-Rocha, Gerardo Ahmad, Siti Aqlima Biosurfactant production and growth kinetics studies of the waste canola oil-degrading bacterium Rhodococcus erythropolis AQ5-07 from Antarctica |
author_facet |
Ibrahim, Salihu Khalil, Khalilah Abdul Zahri, Khadijah Nabilah Mohd Gomez-Fuentes, Claudio Convey, Peter Zulkarnain, Azham Sabri, Suriana Alias, Siti Aisyah González-Rocha, Gerardo Ahmad, Siti Aqlima |
author_sort |
Ibrahim, Salihu |
title |
Biosurfactant production and growth kinetics studies of the waste canola oil-degrading bacterium Rhodococcus erythropolis AQ5-07 from Antarctica |
title_short |
Biosurfactant production and growth kinetics studies of the waste canola oil-degrading bacterium Rhodococcus erythropolis AQ5-07 from Antarctica |
title_full |
Biosurfactant production and growth kinetics studies of the waste canola oil-degrading bacterium Rhodococcus erythropolis AQ5-07 from Antarctica |
title_fullStr |
Biosurfactant production and growth kinetics studies of the waste canola oil-degrading bacterium Rhodococcus erythropolis AQ5-07 from Antarctica |
title_full_unstemmed |
Biosurfactant production and growth kinetics studies of the waste canola oil-degrading bacterium Rhodococcus erythropolis AQ5-07 from Antarctica |
title_sort |
biosurfactant production and growth kinetics studies of the waste canola oil-degrading bacterium rhodococcus erythropolis aq5-07 from antarctica |
publisher |
MDPI |
publishDate |
2020 |
url |
http://nora.nerc.ac.uk/id/eprint/526346/ https://nora.nerc.ac.uk/id/eprint/526346/1/molecules-25-03878-v2.pdf https://www.mdpi.com/1420-3049/25/17/3878 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_relation |
https://nora.nerc.ac.uk/id/eprint/526346/1/molecules-25-03878-v2.pdf Ibrahim, Salihu; Khalil, Khalilah Abdul; Zahri, Khadijah Nabilah Mohd; Gomez-Fuentes, Claudio; Convey, Peter orcid:0000-0001-8497-9903 Zulkarnain, Azham; Sabri, Suriana; Alias, Siti Aisyah; González-Rocha, Gerardo; Ahmad, Siti Aqlima. 2020 Biosurfactant production and growth kinetics studies of the waste canola oil-degrading bacterium Rhodococcus erythropolis AQ5-07 from Antarctica. Molecules, 25 (17), 3878. https://doi.org/10.3390/molecules25173878 <https://doi.org/10.3390/molecules25173878> |
op_rights |
cc_by_4 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3390/molecules25173878 |
container_title |
Molecules |
container_volume |
25 |
container_issue |
17 |
container_start_page |
3878 |
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1766156419148546048 |