Response surface methodology optimisation and kinetics of diesel degradation by a cold-adapted Antarctic bacterium, Arthrobacter sp. strain AQ5-05

Petroleum hydrocarbons, notably diesel oil, are the main energy source for running amenities in the Antarctic region and are the major cause of pollution in this area. Diesel oil spills are one of the major challenges facing management of the Antarctic environment. Bioremediation using bacteria can...

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Published in:Sustainability
Main Authors: Abdulrasheed, Mansur, Zulkharnain, Azham, Zakaria, Nur Nadhirah, Roslee, Ahmad Fareez Ahmed, Khalil, Khalilah Abdul, Napis, Suhaimi, Convey, Peter, Gomez-Fuentes, Claudio, Ahmad, Siti Aqlima
Format: Article in Journal/Newspaper
Language:English
Published: MDPI AG 2020
Subjects:
Online Access:http://nora.nerc.ac.uk/id/eprint/526242/
https://nora.nerc.ac.uk/id/eprint/526242/1/sustainability-12-06966.pdf
https://www.mdpi.com/2071-1050/12/17/6966
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spelling ftnerc:oai:nora.nerc.ac.uk:526242 2023-05-15T13:41:44+02:00 Response surface methodology optimisation and kinetics of diesel degradation by a cold-adapted Antarctic bacterium, Arthrobacter sp. strain AQ5-05 Abdulrasheed, Mansur Zulkharnain, Azham Zakaria, Nur Nadhirah Roslee, Ahmad Fareez Ahmed Khalil, Khalilah Abdul Napis, Suhaimi Convey, Peter Gomez-Fuentes, Claudio Ahmad, Siti Aqlima 2020-08-27 text http://nora.nerc.ac.uk/id/eprint/526242/ https://nora.nerc.ac.uk/id/eprint/526242/1/sustainability-12-06966.pdf https://www.mdpi.com/2071-1050/12/17/6966 en eng MDPI AG https://nora.nerc.ac.uk/id/eprint/526242/1/sustainability-12-06966.pdf Abdulrasheed, Mansur; Zulkharnain, Azham; Zakaria, Nur Nadhirah; Roslee, Ahmad Fareez Ahmed; Khalil, Khalilah Abdul; Napis, Suhaimi; Convey, Peter orcid:0000-0001-8497-9903 Gomez-Fuentes, Claudio; Ahmad, Siti Aqlima. 2020 Response surface methodology optimisation and kinetics of diesel degradation by a cold-adapted Antarctic bacterium, Arthrobacter sp. strain AQ5-05. Sustainability, 12 (17), 6966. 14, pp. https://doi.org/10.3390/su12176966 <https://doi.org/10.3390/su12176966> cc_by_4 CC-BY Publication - Article PeerReviewed 2020 ftnerc https://doi.org/10.3390/su12176966 2023-02-04T19:49:50Z Petroleum hydrocarbons, notably diesel oil, are the main energy source for running amenities in the Antarctic region and are the major cause of pollution in this area. Diesel oil spills are one of the major challenges facing management of the Antarctic environment. Bioremediation using bacteria can be an effective and eco-friendly approach for their remediation. However, since the introduction of non-native organisms, including microorganisms, into the Antarctic or between the distinct biogeographical regions within the continent is not permitted under the Antarctic Treaty, it is crucial to discover native oil-degrading, psychrotolerant microorganisms that can be used in diesel bioremediation. The primary aim of the current study is to optimize the conditions for growth and diesel degradation activity of an Antarctic local bacterium, Arthrobacter sp. strain AQ5-05, using the Plackett-Burman approach and response surface method (RSM) via a central composite design (CCD) approach. Based on this approach, temperature, pH, and salinity were calculated to be optimum at 16.30 °C, pH 7.67 and 1.12% (w/v), respectively. A second order polynomial regression model very accurately represented the experimental figures’ interpretation. These optimized environmental conditions increased diesel degradation from 34.5% (at 10 °C, pH 7.00 and 1.00% (w/v) salinity) to 56.4%. Further investigation of the kinetics of diesel reduction by strain AQ5-05 revealed that the Teissier model had the lowest RMSE and AICC values. The calculated values for the Teissier constants of maximal growth rate, half-saturation rate constant for the maximal growth, and half inhibition constants (μmax, Ks, and Ki), were 0.999 h−1, 1.971% (v/v) and 1.764% (v/v), respectively. The data obtained therefore confirmed the potential application of this cold-tolerant strain in the bioremediation of diesel-contaminated Antarctic soils at low temperature. Article in Journal/Newspaper Antarc* Antarctic Natural Environment Research Council: NERC Open Research Archive Antarctic The Antarctic Sustainability 12 17 6966
institution Open Polar
collection Natural Environment Research Council: NERC Open Research Archive
op_collection_id ftnerc
language English
description Petroleum hydrocarbons, notably diesel oil, are the main energy source for running amenities in the Antarctic region and are the major cause of pollution in this area. Diesel oil spills are one of the major challenges facing management of the Antarctic environment. Bioremediation using bacteria can be an effective and eco-friendly approach for their remediation. However, since the introduction of non-native organisms, including microorganisms, into the Antarctic or between the distinct biogeographical regions within the continent is not permitted under the Antarctic Treaty, it is crucial to discover native oil-degrading, psychrotolerant microorganisms that can be used in diesel bioremediation. The primary aim of the current study is to optimize the conditions for growth and diesel degradation activity of an Antarctic local bacterium, Arthrobacter sp. strain AQ5-05, using the Plackett-Burman approach and response surface method (RSM) via a central composite design (CCD) approach. Based on this approach, temperature, pH, and salinity were calculated to be optimum at 16.30 °C, pH 7.67 and 1.12% (w/v), respectively. A second order polynomial regression model very accurately represented the experimental figures’ interpretation. These optimized environmental conditions increased diesel degradation from 34.5% (at 10 °C, pH 7.00 and 1.00% (w/v) salinity) to 56.4%. Further investigation of the kinetics of diesel reduction by strain AQ5-05 revealed that the Teissier model had the lowest RMSE and AICC values. The calculated values for the Teissier constants of maximal growth rate, half-saturation rate constant for the maximal growth, and half inhibition constants (μmax, Ks, and Ki), were 0.999 h−1, 1.971% (v/v) and 1.764% (v/v), respectively. The data obtained therefore confirmed the potential application of this cold-tolerant strain in the bioremediation of diesel-contaminated Antarctic soils at low temperature.
format Article in Journal/Newspaper
author Abdulrasheed, Mansur
Zulkharnain, Azham
Zakaria, Nur Nadhirah
Roslee, Ahmad Fareez Ahmed
Khalil, Khalilah Abdul
Napis, Suhaimi
Convey, Peter
Gomez-Fuentes, Claudio
Ahmad, Siti Aqlima
spellingShingle Abdulrasheed, Mansur
Zulkharnain, Azham
Zakaria, Nur Nadhirah
Roslee, Ahmad Fareez Ahmed
Khalil, Khalilah Abdul
Napis, Suhaimi
Convey, Peter
Gomez-Fuentes, Claudio
Ahmad, Siti Aqlima
Response surface methodology optimisation and kinetics of diesel degradation by a cold-adapted Antarctic bacterium, Arthrobacter sp. strain AQ5-05
author_facet Abdulrasheed, Mansur
Zulkharnain, Azham
Zakaria, Nur Nadhirah
Roslee, Ahmad Fareez Ahmed
Khalil, Khalilah Abdul
Napis, Suhaimi
Convey, Peter
Gomez-Fuentes, Claudio
Ahmad, Siti Aqlima
author_sort Abdulrasheed, Mansur
title Response surface methodology optimisation and kinetics of diesel degradation by a cold-adapted Antarctic bacterium, Arthrobacter sp. strain AQ5-05
title_short Response surface methodology optimisation and kinetics of diesel degradation by a cold-adapted Antarctic bacterium, Arthrobacter sp. strain AQ5-05
title_full Response surface methodology optimisation and kinetics of diesel degradation by a cold-adapted Antarctic bacterium, Arthrobacter sp. strain AQ5-05
title_fullStr Response surface methodology optimisation and kinetics of diesel degradation by a cold-adapted Antarctic bacterium, Arthrobacter sp. strain AQ5-05
title_full_unstemmed Response surface methodology optimisation and kinetics of diesel degradation by a cold-adapted Antarctic bacterium, Arthrobacter sp. strain AQ5-05
title_sort response surface methodology optimisation and kinetics of diesel degradation by a cold-adapted antarctic bacterium, arthrobacter sp. strain aq5-05
publisher MDPI AG
publishDate 2020
url http://nora.nerc.ac.uk/id/eprint/526242/
https://nora.nerc.ac.uk/id/eprint/526242/1/sustainability-12-06966.pdf
https://www.mdpi.com/2071-1050/12/17/6966
geographic Antarctic
The Antarctic
geographic_facet Antarctic
The Antarctic
genre Antarc*
Antarctic
genre_facet Antarc*
Antarctic
op_relation https://nora.nerc.ac.uk/id/eprint/526242/1/sustainability-12-06966.pdf
Abdulrasheed, Mansur; Zulkharnain, Azham; Zakaria, Nur Nadhirah; Roslee, Ahmad Fareez Ahmed; Khalil, Khalilah Abdul; Napis, Suhaimi; Convey, Peter orcid:0000-0001-8497-9903
Gomez-Fuentes, Claudio; Ahmad, Siti Aqlima. 2020 Response surface methodology optimisation and kinetics of diesel degradation by a cold-adapted Antarctic bacterium, Arthrobacter sp. strain AQ5-05. Sustainability, 12 (17), 6966. 14, pp. https://doi.org/10.3390/su12176966 <https://doi.org/10.3390/su12176966>
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op_doi https://doi.org/10.3390/su12176966
container_title Sustainability
container_volume 12
container_issue 17
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