Discovery of microcystin-producing Anagnostidinema pseudacutissimum from cryopreserved Antarctic cyanobacterial mats
Microcystins (MCs) are secondary metabolites produced by cyanobacteria and have been well-documented in temperate and tropical regions. However, knowledge of the production of MCs in extremely cold environments is still in its infancy. Recently, examination of 100-year-old Antarctic cyanobacterial m...
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ftnerc:oai:nora.nerc.ac.uk:525158 2023-05-15T13:41:44+02:00 Discovery of microcystin-producing Anagnostidinema pseudacutissimum from cryopreserved Antarctic cyanobacterial mats Zaki, Nur Syazana Merican, Faradina Muangmai, Narongrit Convey, Peter Broady, Paul 2020-03 http://nora.nerc.ac.uk/id/eprint/525158/ https://www.sciencedirect.com/science/article/pii/S1568988320300809 unknown Elsevier Zaki, Nur Syazana; Merican, Faradina; Muangmai, Narongrit; Convey, Peter orcid:0000-0001-8497-9903 Broady, Paul. 2020 Discovery of microcystin-producing Anagnostidinema pseudacutissimum from cryopreserved Antarctic cyanobacterial mats. Harmful Algae, 93, 101800. https://doi.org/10.1016/j.hal.2020.101800 <https://doi.org/10.1016/j.hal.2020.101800> Publication - Article PeerReviewed 2020 ftnerc https://doi.org/10.1016/j.hal.2020.101800 2023-02-04T19:49:15Z Microcystins (MCs) are secondary metabolites produced by cyanobacteria and have been well-documented in temperate and tropical regions. However, knowledge of the production of MCs in extremely cold environments is still in its infancy. Recently, examination of 100-year-old Antarctic cyanobacterial mats collected from Ross Island and the McMurdo Ice Shelf during Captain R.F. Scott's Discovery Expedition revealed that the presence of MCs in Antarctica is not a new phenomenon. Here, morphological and molecular phylogenetic analyses are used to identify a new microcystin-producing freshwater cyanobacterium, Anagnostidinema pseudacutissimum. The strain was isolated from a deep-frozen (−15 °C) sample collected from a red-brown cyanobacterial mat in a frozen pond at Cape Crozier (Ross Island, continental Antarctica) in 1984–1985. Amplification of the mcyE gene fragment involved in microcystin biosynthesis from A. pseudacutissimum confirmed that it is identical to the sequence from other known microcystin-producing cyanobacteria. Analysis of extracts from this A. pseudacutissimum strain by HPLC-MS/MS confirmed the presence of MC-LR and -YR at concentrations of 0.60 μg/L and MC-RR at concentrations of 0.20 μg/L. This is the first report of microcystin production from a species of Anagnostidinema from Antarctica. Article in Journal/Newspaper Antarc* Antarctic Antarctica Ice Shelf McMurdo Ice Shelf Ross Island Natural Environment Research Council: NERC Open Research Archive Antarctic Ross Island McMurdo Ice Shelf ENVELOPE(166.500,166.500,-78.000,-78.000) Crozier ENVELOPE(169.400,169.400,-77.517,-77.517) Cape Crozier ENVELOPE(169.400,169.400,-77.517,-77.517) Harmful Algae 93 101800 |
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Open Polar |
collection |
Natural Environment Research Council: NERC Open Research Archive |
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ftnerc |
language |
unknown |
description |
Microcystins (MCs) are secondary metabolites produced by cyanobacteria and have been well-documented in temperate and tropical regions. However, knowledge of the production of MCs in extremely cold environments is still in its infancy. Recently, examination of 100-year-old Antarctic cyanobacterial mats collected from Ross Island and the McMurdo Ice Shelf during Captain R.F. Scott's Discovery Expedition revealed that the presence of MCs in Antarctica is not a new phenomenon. Here, morphological and molecular phylogenetic analyses are used to identify a new microcystin-producing freshwater cyanobacterium, Anagnostidinema pseudacutissimum. The strain was isolated from a deep-frozen (−15 °C) sample collected from a red-brown cyanobacterial mat in a frozen pond at Cape Crozier (Ross Island, continental Antarctica) in 1984–1985. Amplification of the mcyE gene fragment involved in microcystin biosynthesis from A. pseudacutissimum confirmed that it is identical to the sequence from other known microcystin-producing cyanobacteria. Analysis of extracts from this A. pseudacutissimum strain by HPLC-MS/MS confirmed the presence of MC-LR and -YR at concentrations of 0.60 μg/L and MC-RR at concentrations of 0.20 μg/L. This is the first report of microcystin production from a species of Anagnostidinema from Antarctica. |
format |
Article in Journal/Newspaper |
author |
Zaki, Nur Syazana Merican, Faradina Muangmai, Narongrit Convey, Peter Broady, Paul |
spellingShingle |
Zaki, Nur Syazana Merican, Faradina Muangmai, Narongrit Convey, Peter Broady, Paul Discovery of microcystin-producing Anagnostidinema pseudacutissimum from cryopreserved Antarctic cyanobacterial mats |
author_facet |
Zaki, Nur Syazana Merican, Faradina Muangmai, Narongrit Convey, Peter Broady, Paul |
author_sort |
Zaki, Nur Syazana |
title |
Discovery of microcystin-producing Anagnostidinema pseudacutissimum from cryopreserved Antarctic cyanobacterial mats |
title_short |
Discovery of microcystin-producing Anagnostidinema pseudacutissimum from cryopreserved Antarctic cyanobacterial mats |
title_full |
Discovery of microcystin-producing Anagnostidinema pseudacutissimum from cryopreserved Antarctic cyanobacterial mats |
title_fullStr |
Discovery of microcystin-producing Anagnostidinema pseudacutissimum from cryopreserved Antarctic cyanobacterial mats |
title_full_unstemmed |
Discovery of microcystin-producing Anagnostidinema pseudacutissimum from cryopreserved Antarctic cyanobacterial mats |
title_sort |
discovery of microcystin-producing anagnostidinema pseudacutissimum from cryopreserved antarctic cyanobacterial mats |
publisher |
Elsevier |
publishDate |
2020 |
url |
http://nora.nerc.ac.uk/id/eprint/525158/ https://www.sciencedirect.com/science/article/pii/S1568988320300809 |
long_lat |
ENVELOPE(166.500,166.500,-78.000,-78.000) ENVELOPE(169.400,169.400,-77.517,-77.517) ENVELOPE(169.400,169.400,-77.517,-77.517) |
geographic |
Antarctic Ross Island McMurdo Ice Shelf Crozier Cape Crozier |
geographic_facet |
Antarctic Ross Island McMurdo Ice Shelf Crozier Cape Crozier |
genre |
Antarc* Antarctic Antarctica Ice Shelf McMurdo Ice Shelf Ross Island |
genre_facet |
Antarc* Antarctic Antarctica Ice Shelf McMurdo Ice Shelf Ross Island |
op_relation |
Zaki, Nur Syazana; Merican, Faradina; Muangmai, Narongrit; Convey, Peter orcid:0000-0001-8497-9903 Broady, Paul. 2020 Discovery of microcystin-producing Anagnostidinema pseudacutissimum from cryopreserved Antarctic cyanobacterial mats. Harmful Algae, 93, 101800. https://doi.org/10.1016/j.hal.2020.101800 <https://doi.org/10.1016/j.hal.2020.101800> |
op_doi |
https://doi.org/10.1016/j.hal.2020.101800 |
container_title |
Harmful Algae |
container_volume |
93 |
container_start_page |
101800 |
_version_ |
1766155850682990592 |